Biomedical subjects
Publications and source records attributed to L M Gakh.
The course of bronchitis with clinical manifestations of allergy is characterized by certain cytochemical features which (as compared with bronchitis without allergy) show an increase of deoxyribonucleic acid, increased permeability of the intracellular membranes of polynuclear leucocytes and lymphocytes, reduction of the content of cationic proteins and activity of acid phosphatase in the polynuclear leucocytes of the blood.
Specific (tuberculin, adrenalin) and nonspecific (ultrasound, phenol) in vitro exposures of the blood improve the diagnostic informativeness of the red cell sedimentation rate measurements. Similarity of the results of measurements, carried out by the suggested and the reference techniques in more than 550 patients, gives grounds to employ the suggested modifications of tuberculin diagnosis, of detecting a beta-adrenodependent bronchospasm, of assessing the pattern and the activity of an inflammation in pneumonias. Practical and statistical rationale for the suggested methods is given.
In vitro exposure of the blood to UV irradiation and ultrasound changes the content of cationic proteins in polynuclear leukocytes. The difference (vs. the intact blood) is diagnostically valuable, for it helps locate the inflammation in the lungs, determine the origin of the bronchial spasm, and predict resistance to relapses of pulmonary inflammations.
The authors emphasize informative inadequacy of traditional laboratory diagnosis and anti-inflammatory efficacy of treatment of chronic bronchitis. It is proposed to use a new method (Sigma-ESR) reflecting the activity and specificity of tissue processes in the lungs during inflammation that substantiates objectification of diagnosis. The technique is described.
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Lung inflammation in Wistar rats was induced by transthoracic inoculation of live and inactivated cultures of Staphylococcus aureus P209. In the former case the rats received phitohemagglutinin and cyclophosphane. A specific nature of inflammatory processes in these conditions was confirmed by histological, histo-cytochemical and bacterioscopic indexes; humoral and cellular immune responses being similar. The data show no direct correlation between inflammatory and immune reactions.
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Two-week pneumonia was induced in Wistar rats by introduction of a foreign body into the trachea under the effect of heparin, cyclophosphamide, phytohemagglutinin, and liquid paraffin. The morphological and immunological features of the processes were studied. It was discovered that modification of the inflammatory process provoked by the use of the above-mentioned substances is not connected with their immunoregulatory effects or with the induced changes in the humoral and cellular immune response. The data obtained are regarded as evidence for the possibility of the absence of the relationship between the development of the inflammatory process and the status of immunity.
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Cytochemical study of NAD-diaphorase and acid phosphatase (mitochondria and lysosomes markers) in the cells of the abdominal cavity exudate with aseptic inflammation and additional action of polyvinylpyridne-N-oxide with molecular weight in the range of from 2000 to 150 000 formed a background for demonstrating the possibility of stabilizing by means of the latter compound of mitochondrial and lysosomal membranes of macrophages, neutrophils and lymphocytes. The most pronounced stabilizing effect is produced by polyvinylpyridine-N-oxide with a molecular weight of 50 000 with its intramuscular introduction in a dose of 100 mg/kg.