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Biomedical subjects

L M Gilbert

Publications and source records attributed to L M Gilbert.

27 records · Page 2Linked to original sources
PubMed1979

Zinc retention and losses of zinc in sweat by preadolescent girls.

Preadolescent girls, ages 7 to 9 years, were fed levels of zinc ranging from 5.61 to 14.61 mg/day during an 18-day metabolic study. All other nutrients were provided at or above Recommended Dietary Allowance levels. The sweat loss of zinc was measured using an arm-bag method and whole body zinc loss was calculated from whole body nitrogen and arm loss of nitrogen and zinc. Fecal zinc reflected dietary zinc, but urinary zinc remained relatively stable. The mean daily loss of zinc through sweat was calculated to be 1.43 mg. Zinc retentions were not significantly different (P greater than 0.05) though the retention increased generally with intake of zinc. A comparison of these data with previously reported studies indicated that the absorption of zinc was usually in the range of 20 to 30%. Based on sweat losses reported by others and found in this study and on usual absorption of zinc, it appears that diets should provide a minimum of 7 mg of zinc daily for growing children.

Child↗
PubMed1975

Evaluation of the precision of difference chromatography.

Difference chromatography, a method of chromatography in which elution volumes are compared directly, has been tested for precision under conditions used for estimating the relative dissociation of human oxyhaemoglobin and methaemoglobin and their hybrid. The test was carried out by first establishing a steady flow of haemoglobin through a column of gel particles, and then interrupting the flow temporarily by injecting a known weight of buffer solution, which thus simulated a small difference in elution volume. The dip in absorbance on the elution record was evaluated by digital methods. Statistical analysis of the results showed that the injected weight was estimated from the elution record with a standard error of plus or minus 0.02 g. Since the elution weight was about 50 g, the precision of difference chromatography must approach that of difference ultracentrifugation, and has obvious potential for the accurate comparison of the molecular weights of polypeptides in strongly dispersing media.

Chromatography, Gel↗
PubMed1975

A blaze-dry spreading procedure for the electron microscopy of chromosomes from acid alcohol-fixed human lymphocytes.

A procedure is described for the blaze-drying of human lymphocyte chromosomes on carbonized Parlodion film. Films are prepared by applying Parlodion solution to sheets of freshly cleaved mica. Damage to the film during blaze-drying is prevented by chilling the mica sheets on dry ice before flaming. After spreading, the film and metaphases are floated free from the mica and transferred to a slide of Formav-coated electron microscope grids. The resulting preparations yield complete metaphase spreads and banded chromosomes morphologically similar to those observed with the light microscope.

Azure Stains↗
PubMedDate not supplied

Gonorrheal disease among children in a university hospital.

In the current veneral disease epidemic the pediatrician may observe every clinical expression of gonococcal infection. Review of outpatient records at the University of Maryland Pediatric Clinic (Baltimore, Md.) showed frequent utilization of this health-care facility by girls with genitourinary complaints and a high rate of recovery of Neisseria gonorrhoeae from those patients from whom specimens were obtained for culture. The adolescent girl is the most likely pediatric patient to have genitourinary complaints that indicate a diagnosis of gonorrhea. Overall, 16.3% of girls and 37.2% of boys who had speciments cultured for N. gonorrhoeae had positive results. Although the older teenager (15--18 years of age) was most likely to have a positive culture, high rates comparable to those reported in venereal disease clinics were found for all age groups. Half of the cases of gonorrhea in girls aged 15 to 18 years took the clinical form of pelvic inflammatory disease. Most gonococcal infections in prepubertal children were in girls 2 to 5 years of age and resulted from presumed sexual abuse. Vulvovaginitis was the initial symptom in all cases. Prepubertal boys infected with N. gonorrhoeae all had urethritis; three of seven admitted to voluntary sexual activity.

Adolescent↗