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L M HENDERSON

Publications and source records attributed to L M HENDERSON.

At least 19 recordsLinked to original sources
PubMed1965

INCORPORATION OF HYDROXYLYSINE INTO THE CELL WALL AND A CELL-WALL PRECURSOR IN STAPHYLOCOCCUS AUREUS.

Smith, W. Grady (University of Minnesota, St. Paul), Daniel P. Gilboe, and L. M. Henderson. Incorporation of hydroxylysine into the cell wall and a cell-wall precursor in Staphylococcus aureus. J. Bacteriol. 89:136-140. 1965.-Recent work has shown that hydroxylysine can substitute for lysine in cell-wall synthesis of Streptococcus faecalis, apparently becoming incorporated into cell-wall mucopeptide. This paper extends these observations to investigate the metabolism of hydroxylysine in Staphylococcus aureus, an organism from which sufficiently large quantities of cell-wall precursors. uridine diphosphate-N-acetylmuramyl peptides, could be obtained. Hydroxylysine has been shown to be incorporated into the cell-wall precursor uridine diphosphate-N-acetylmuramyl l-ala.d-glu. l-lys.d-ala.d-ala from S. aureus (Copenhagen) apparently in lieu of lysine. Hydroxylysine was also incorporated into the cell-wall mucopeptides of S. aureus in resting cultures. This incorporation was inhibited by penicillin or lysine, but not by chloramphenicol. Hydroxylysine had little effect on the incorporation of lysine into S. aureus. Hydroxylysine acted as a growth inhibitor in this organism; the inhibition was reversed by lysine.

Alanine↗
PubMed1963

Tryptophan-niacin relationship in Xanthomonas pruni.

Wilson, R. G. (Oklahoma State University, Stillwater) and L. M. Henderson. Tryptophan-niacin relationship in Xanthomonas pruni. J. Bacteriol. 85:221-229. 1963.-The observation that Xanthomonas pruni, a bacterial pathogen for the peach, requires niacin for growth and can use tryptophan or 3-hydroxyanthranilic acid as a substitute was confirmed. To determine whether niacin is synthesized via the tryptophan-3-hydroxyanthranilic acid pathway, experiments using labeled metabolites were undertaken. Labeled tryptophan, 3-hydroxyanthranilic acid, quinolinic acid, and nicotinic acid were supplied in the basal medium in amounts sufficient to insure maximal growth. Nicotinic and quinolinic acids were isolated from the cells after the growth period. The isotope was incorporated from the first three labeled compounds into niacin with dilutions approximately the same in all cases, ranging from 7.6 to 17.1. The dilution of isotopic niacin was 3.1- to 5.9-fold. Only labeled quinolinic acid gave rise to labeled quinolinic acid in the cell, but this acid gave rise to niacin with 10- to 12-fold reduction in specific activity. The results indicate that if quinolinate participates as an obligatory intermediate in the synthesis of niacin from tryptophan, its concentration within the cell is very small and it does not equilibrate readily with exogenous quinolinate. The results confirm the conclusion, drawn from growth studies, that niacin is needed to permit tryptophan synthesis at a sufficient rate to promote growth. In the absence of an external source of niacin, tryptophan or some of its metabolites can promote growth by acting as precursors of niacin.

Niacin↗