Biomedical subjects
Publications and source records attributed to L M Lopes.
Most soluble protein antigens are poor at priming class I-restricted cytotoxic cell activity. In this study we show that incorporation of one protein, ovalbumin, into liposomes converts this antigen into an effective stimulator of antigen-specific cytotoxicity. The mechanisms responsible for this phenomenon are discussed.
PURPOSE: To study the pathogenesis, evolution and prognosis of the complete heart block of the fetus. METHODS: Bidimensional echocardiography associated to M-mode and doppler was performed in 600 patients. All cases of congenital heart block were referred because the fetuses presented hydrops, bradycardia and/or cardiac malformation suspected by routine ultrasound. RESULTS: Isolated heart block was found in 6 fetuses (5 cases of complete type and 1 case of 2nd degree type 2:1). Heart block associated with complex cardiac disease and left atrial isomerism was found in 6 fetuses with no survivors (5 cases of complete type and 1 case of 2nd degree type 2:1). Heart block associated with atrioventricular discordante was found in 1 case. CONCLUSION: The findings of this study agree the literature about the relation between maternal anti-RO antibodies and isolated complete heart block. We also found a poor prognosis in the group with heart block and complex cardiac malformations.
Nine maxillary premolars were restored with composite resin inlays involving large intracoronal cavity preparations. Buccal and lingual bonded strain guages measured the cuspal flexure under a carefully controlled application of occlusal force. The intact tooth was compared with the corresponding preparation and final restoration. The preparation itself greatly reduced the coronal rigidity, but this was completely recovered in the restored tooth, within the functional force of 111 N. A stiffness ratio showed a 97% recovery. From the point of view of cuspal strength, this may mean that larger intracoronal restorations are feasible with this type of restoration. However, other factors, such as chairside time and complexity, and material properties, such as occlusal wear, have to be taken into consideration.
A significant polyclonal activation of B lymphocytes was observed during experimental infection of C57BL/10J mice with Schistosoma mansoni. The isotypic pattern of this expansion, assessed by the Protein-A plaque-forming cell method, was compared with and found to differ from those occurring after infection by Trypanosoma cruzi or injection of bacterial LPS. In the infection of S. mansoni an early expansion of most immunoglobulin isotypes occurs together with a late, sustained expansion of IgG1-secreting cells. High levels of polyclonal B cell activation were observed after adoptive transfer of spleen cells from infected mice to isogenic recipients pre-treated with hydroxyurea.
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PURPOSE: To detect in prenatal life fetal arrhythmia and congenital heart disease. We performed fetal echocardiography following classical indications according to the literature. PATIENTS AND METHODS: Two-dimensional echocardiography associated with M-mode and Doppler was performed in 200 patients according to the indications: maternal diabetes, fetal arrhythmia, maternal congenital heart disease, previous fetus with cardiac defect, intrauterine growth retardation, nonimmune fetal hydrops, rubeola, isoimmunization, fetal malformation by ultrasound, polyhydramnios, gemelarity, oligohydramnios, maternal ingestion of drugs, lupus and others. RESULTS: Structural abnormalities of the heart were found in 6 cases and associated with bradiarrhythmia in 5 cases (total of 5.5%). Fetal arrhythmias without cardiac malformation were found in 35 cases (17.5%) and premature atrial contractions were the most frequent. CONCLUSION: The findings in this study indicate that it is possible the accurate definition of the cardiac anatomy and function. We have emphasized the method trying to spread it, objecting to improve assistance to the fetus and newborn with arrhythmias and/or congenital heart disease.
1. We show that mouse strains differ widely in susceptibility to tolerance induction and/or immunization (priming) following contact of protein antigens (ovalbumin, human or bovine gamma globulins) with different mucosal surfaces. 2. When compared to a control group pretreated with saline, mice pretreated by the oral (intragastric) route with antigen became significantly less responsive to subsequent parenteral immunization (i.e., tolerant). This was observed in most, but not all, antigen/strain combinations. 3. Similar, although less prominent changes were induced by pretreatments with antigen by the ocular (conjunctival) route. 4. No significant effects were observed following pretreatments by the nasal, vaginal or rectal routes. 5. Genes present in strains selected for multispecific "high" or "low" responsiveness are included among those involved in tolerance induction following mucosal contacts with protein antigens.
Inbred mouse strains vary widely in their susceptibility to the induction of tolerance following oral (intragastric) administration of ovalbumin. Marked differences were found between strains that form a congenic pair differing at the H-2 complex: C3H/HeJ (H-2k) and C3H.SW (H-2b)-which were very susceptible and resistant to tolerance induction, respectively. In contrast, no significant differences were found between A/J (H-2a) and A.BY (H-2b) congenics, which were both susceptible, nor among C57BL/10J congenics, which were uniformly resistant to tolerance induction. We conclude that H-2-linked genes determine tolerance susceptibility in conjunction with background genes.
Classic cavity preparations involve significant removal of tooth structure. This has the potential for reducing the overall resistance of the tooth and puts the tooth at risk for fracture in the future. In this paper the pertinent scientific literature is reviewed and various techniques of evaluating the resistance of teeth to fracture are discussed in comparison to a new method developed at the University of Minnesota. Based on the results obtained by this new method, the authors present conclusions supporting the clinical advantages of using a more conservative restorative treatment.
The authors present a quite unused technique that helps to simplify the cavity preparation in Operative Dentistry. As a sequence of his investigations that established a clear distinction between hardness and bacterial invasion of dentin, Fusayama searched for a simple clinical method that could avoid excessive removal of remineralizable dentin. The use of "Caries Detector" may provide in few minutes an immediate clinical distinction between "infected dentin" and "remineralizable dentin", by the direct application of a selective dye. The fundamental, mechanism of action, and the application technique of this product are described.
The author discusses the different philosophies concerning the emergency treatment of the acute apical abcess, proposing the corresponding attitudes in this case: immediate transradicular drainage, complete chemo-mechanical cleaning of the root canal and closing the access to the root canal as soon as possible. In the most serious situations we can make an incision for drainage through the soft tissues. Pain and infection control.