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Biomedical subjects

L M Webber

Publications and source records attributed to L M Webber.

11 recordsLinked to original sources
PubMed1990

Fc receptor gene translocation in a t(1;19) pre-B ALL cell line.

We have recently mapped the human FCGR2 gene to chromosome 1 bands q23-q24. In situ hybridization of FCGR2 cDNA with a cell line containing a t(1:19)(q23;p13) derived from a patient with pre-B ALL has allowed a more accurate localization of this gene to chromosome 1 band q23. Furthermore, this study indicated a splitting of the FCGR2 gene or gene cluster by the t(1;19). However, Southern analysis showed no genetic rearrangement when compared with a karyotypically normal Epstein-Barr virus (EBV)-transformed cell line from the same patient. This suggests that the translocation breakpoint does not occur within the coding region of this gene.

Chromosome Mapping↗
PubMed1988

Isodicentric X chromosomes involving the Xq13 breakpoint in myelodysplasia and acute nonlymphocytic leukemia.

Many nonrandom chromosome abnormalities, usually autosomal in nature, have been found to be associated with specific types of acute nonlymphocytic leukemia (ANLL) and myelodysplastic syndromes (MDS). Specific abnormalities involving the sex chromosomes are rare. We have recently identified a structural abnormality of the X chromosome, for example, idic(X)(q13) in three patients: two with MDS progressing to ANLL and one with ANLL de novo. All three patients were elderly females with a very aggressive form of ANLL. Six other patients with a similar abnormality have been discovered in the literature; all having either MDS or ANLL and a short survival. It is suggested that the abnormality identifies a subset of MDS and ANLL occurring in elderly females.

Acute Disease↗
PubMed1987

Localization of the G-CSF gene on chromosome 17 proximal to the breakpoint in the t(15;17) in acute promyelocytic leukemia.

The human granulocyte-colony stimulating factor gene (G-CSF) is localized at 17q11.2-q21, the region of one of the breakpoints in the 15;17 chromosome translocation specific for acute promyelocytic leukemia (APL). As G-CSF induces differentiation and loss of tumorigenicity in myeloid leukemic cells or cell lines, it was possible that the translocation in APL involved the DNA of the G-CSF coding region or its regulatory region. In situ hybridization to chromosomes with the t(15;17) from patients with the APL translocation using a G-CSF cDNA clone revealed that the coding region of this gene is proximal to the t(15;17) breakpoint on chromosome 17. Southern analysis of DNA from patients with the APL translocation showed no differences in hybridization between normal and leukemic cells. These results indicate that the G-CSF coding sequence is not disrupted by the chromosomal rearrangement characteristic of APL.

Acute Disease↗
PubMed1985

Human tumour cell lines established using clonal agar culture.

Four families of human tumour cell lines--one of uterine, and three of ovarian origin--were established at early passage level from primary biopsy specimens of terminal patients by the propagation of anchorage-independent agar clones in liquid culture. All cell lines exhibited unique and stable characteristics and retained their ability to clone in agar. However, considerable heterogeneity was evident in clonogenic capacity, karyotype, and responsiveness to growth-promoting substances even among progeny of single agar clones isolated from the one biopsy specimen. These cell lines will be made available for further study upon request.

Agar↗
PubMed1975

Oxygen dissociation after transfusion of blood stored in ACD or CPD solution.

In 20 patients undergoing open-heart surgery, 2,3-diphosphoglycerate (2,3-DPG) concentrations, oxygen affinity of hemoglobin (Po2 at half saturation of hemoglobin with oxygen [P50]), hemoglobin concentration, and pH were measured repeatedly. Measurements were made before and at various times after open-heart surgery and replacement of blood loss with blood stored in acid-citrate-dextrose (ACD) or citrate-phosphate-dextrose (CPD) solutions for less than 72 hours (10 cases per group). Infusion of ACD blood caused P50 and 2,3-DPG concentration to decrease significantly after the operation. The infusion of blood stored in CPD did not significantly increase the oxygen affinity. No significant changes in hemoglobin concentration or pH were observed immediately after the operation in either group. To compensate for the increased oxygen affinity, there must be a rise in cardiac output or more likely a decrease in venous Po2. The transfusion of CPD blood, therefore, is more favorable in terms of oxygen supply, particularly in patients who have had cardiac surgery.

Adult↗