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L Malkin

Publications and source records attributed to L Malkin.

9 recordsLinked to original sources
PubMed2005

Asymmetry and diversity of dermatoglyphic traits: population comparison in five endogamous groups of West Bengal, India.

Five different endogamous populations encompassing the main social ranks in the caste hierarchy of West Bengal, India were analyzed. To compare variability in populations with contrasting ethnohistorical backgrounds, analysis of variance, Scheffe's test and cluster analysis were performed, as based on dermatoglyphic variables, namely, 22 quantitative traits and 36 indices of diversity and asymmetry. The present study reveals that: 1. Overall disparities among the 5 populations are expressed only in finger ridge counts on the Ist and Vth digits and PII, in a-b ridge counts, in endings of main lines A and D, and in MLI on the palms; 2. Heterogeneity is greater in fluctuating asymmetry than in directional asymmetry; 3. There is a greater heterogeneity in the 22 quantitative traits than in the 36 indices of diversity and asymmetry, with females contributing more than the males; 4. The highest contribution to population variation is by Lodha among five populations; 5. Inter-group variations are homogeneous in most of the variables, which does not correspond with the relationships to caste hierarchy of these populations; 6. The dendrograms based on dermatoglyphic variables demonstrate that the traditional grouping of Indian populations, based on caste hierarchy, may not be a reflection of their genetic origin, in that the pattern of clustering corresponded best with the known ethnohistorical records of the studied populations; 7. Hence, dermatoglyphic affinities may prove quite useful in tracing the ethnohistorical background of populations.

Dermatoglyphics↗
PubMed2001

MIB-1 monoclonal antibody to determine proliferative activity of Ki-67 antigen as an adjunct to the histopathologic differential diagnosis of Spitz nevi.

BACKGROUND: The histopathologic differential diagnosis of Spitz nevus (SN) from malignant melanoma (MM) may be difficult. OBJECTIVE: Our purpose was to determine the staining pattern and usefulness of MIB-1 antibody, which recognizes Ki-67 antigen in formalin-fixed, paraffin-embedded tissue, as an adjunct to the histopathologic differential diagnosis of SN. METHODS: Twenty-five compound SNs, 27 MMs, and 26 compound nondysplastic melanocytic nevi (MNs) were immunostained with the MIB-1 antibody. RESULTS: The mean counts of MIB-1--stained tumor cells of the epidermal and dermal components, both alone and together, were significantly lower in SNs and MNs than in MMs (P <.0001). The dermal counts showed the best discriminating power. In addition, the mean dermal/epidermal count ratios for MIB-1 in SNs and MNs (0.25 and 0.23, respectively) were significantly lower than the corresponding ratio (0.94) in MMs (P <.0001). CONCLUSION: MIB-1-stained tumor cell counts, especially of the dermal component, and dermal/epidermal MIB-1 count ratios may be helpful as an adjunct to the histopathologic differential diagnosis of SN.

Antibodies, Monoclonal↗
PubMed2000

[Solid and papillary pancreatic neoplasm].

Solid and papillary neoplasm of the pancreas is an interesting and rare malignant tumor. It occurs most commonly in young women. It was first described in 1959 and since then has been referred to by different names, including solid and cystic tumor, solid and cystic epithelial neoplasms, and others. Its malignant potential is low and metastasis is very rare. Treatment includes partial pancreatectomy with full resection of the tumor. The prognosis is generally very good. We present 3 women (aged 17, 19, 39) diagnosed and treated for solid and papillary neoplasm of the pancreas. The unique clinical, histological, and epidemiological characteristics of this tumor are detailed.

Adolescent↗
PubMed1999

Comparison of CMV, RSV, SV40 viral and Vlambda1 cellular promoters in B and T lymphoid and non-lymphoid cell lines.

Determining the activity of viral and cellular regulatory elements in B or T lymphoid cell lines would facilitate appropriate utilization of the regulatory sequences for gene transfer- and expression-dependent applications. We have compared the activity of the CMV, RSV and SV40 viral promoter/enhancers as well as the Vlambda1 cellular promoter, in three B cell lines (REH, SMS-SB, C3P), three T cell lines (CEM, Jurkat, ST-F10), and two non-lymphoid cell lines (K-562, HeLa) using the luciferase reporter gene. In B cell lines, the activity of the CMV promoter/enhancer construct was the highest ranging from 10- to 113-fold greater than that of SV40. In contrast, in T cell lines the RSV promoter/enhancer activity was 11-65-fold higher than that of SV40. The Vlambda1 promoter activity was close to that of SV40 promoter/enhancer in most of the cell lines tested. We conclude that CMV and RSV promoter/enhancers contain stronger regulatory elements than do the SV40 and Vlambda1 for expression of genes in lymphoid cell lines.

Avian Sarcoma Viruses↗
PubMed1998

Brenner tumor of the vagina.

Two cases of Brenner tumor of the vagina were encountered in postmenopausal asymptomatic women who presented with polypoid lesions measuring 0.5 and 1.2 cm, respectively, in the upper and mid-vagina. DNA flow cytometry in one tumor showed a diploid histogram, and Ki-67 (MIB-1) proliferation marker was very low in both tumors. To the best of our knowledge, only two vaginal Brenner tumors have been previously reported. Transition between the vaginal surface epithelium and the tumor in one of our cases may support a metaplastic mullerian origin for these tumors.

Aged↗
PubMed1997

Cloning and characterization of the human recombination activating gene 1 (RAG1) and RAG2 promoter regions.

Recombination activating gene 1 (RAG1) and RAG2 are the essential and tissue-specific components of V(D)J recombination. We have characterized the genomic organization of the human RAG locus, mapped the transcriptional initiation sites, and partially sequenced and performed functional reporter assays on the 5' flanking regions of human RAG1 and RAG2. Transcription initiation sites were mapped by rapid amplification of 5' cDNA ends, primer extension, and/or RNase protection in normal thymocytes, three pre-B cell lines, and a mature B cell line. A single promoter region was used for RAG1 transcription. In contrast, transcription of RAG2 initiates at two distinct regions of the genome. The 5'-flanking region of the human RAG2 gene is TATA-less; however, there is a GATAA consensus at position -34 with respect to the major transcriptional initiation site of RAG1. Promoter regions of human RAG1 and RAG2 are active in both lymphoid and nonlymphoid cell lines, suggesting that an outside regulatory element is probably involved in the tissue-specific transcriptional regulation of the RAG genes.

Base Sequence↗
PubMedDate not supplied

Sarcoma of the maxillofacial region: follow-up of 25 cases.

BACKGROUND: Maxillofacial sarcomas are rare tumors, varying according to their type, grade, site of occurrence, response to treatment and prognosis. Approximately 10% of all cases of osteosarcoma, a subtype with a particularly poor prognosis, occur in the head and neck. PATIENTS AND METHODS: During the 35 years from 1963 until 1997, the 25 patients treated for sarcomas at our center were identified and evaluated according to a variety of parameters. RESULTS: Overall 2- and 5-year survival rates were 72% and 60%, respectively. Sarcomas were found most commonly in the mandible, maxilla and tongue. Survival was not significantly correlated with gender, ethnic origin or histopathological staging, but significant correlation was found between type of sarcoma and survival whilst younger patients had better survival rates. CONCLUSION: Advanced facial reconstruction methods may enable the performance of more radical ablative surgery, thereby improving survival outcome for head and neck osteosarcoma patients. Osteosarcoma appears to be as lethal in the maxillofacial region as in the extremities.

Adolescent↗