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L Mastrolia

Publications and source records attributed to L Mastrolia.

8 recordsLinked to original sources

Fine localization of the acetylcholinesterase activity in somatic and germ cells during the morphogenesis of chick ovarian cortex.

The ultracytochemical localization of the enzyme acetylcholinesterase (AChE) was studied in the germinal epithelium and cortex during chick ovarian morphogenesis at the 7, 12.5, 14 and 19 day of incubation. The results evidenced at day 7 the presence of the enzyme in some somatic cells, both "dark" and "light" and in some gonocytes. The reaction appears also in some tracts between these types of cells. At day 12.5 the reaction is present in some somatic cells only towards the deepest zone of the cortex, in many oogonia and oocytes and in some tracts between germ and somatic cells. At day 14 and day 19 the various cell categories of the cortex are negative for the reaction. The significance of the presence of the enzyme is discussed in relation to an embryonic cholinergic system active during morphogenesis. Regarding the presence of the enzyme in germ cells, the positivity in endoplasmic reticulum cisternae associated to mitochondria may suggest an implication of the enzymatic activity in the proliferation and transformation of these organelles.

Acetylcholinesterase

Comparative data on acetylcholinesterase cytochemistry in various chromaffin cell types of Discoglossus pictus (Amphibia, Anura).

The cytochemical localization of acetylcholinesterase (AChE) activity was studied in the adrenal chromaffin cells of Discoglossus pictus. Reaction end-products were associated with all types of chromaffin cells, i.e. adrenaline (A), noradrenaline (N) and small granule chromaffin (SGC-A, SGC-N) cells, and nervous elements present in the gland. The SGC-A and SGC-N showed the same intensity of AChE reaction in A and N cells, respectively. On the whole, the A and SGC-A cells were more reactive than the N and SGC-N cells. The functional role of the SGC cells is discussed on the basis of the cytochemical results.

Acetylcholinesterase

[Some observations on the formation of the primary tunica albuginea in the ovary of the chick embryo (author's transl)].

The ovarian cortex of chicken embryos of 10 to 14 days of incubation was observed in order to study the formation of the primary tunica albuginea and to discern in its cells an ultrastructural confirmation of an eventual steroidogenic capacity which has been proposed by some authors. From observations at the electron microscope, it is probable that the mesenchyme-like cells which constitute the primary tunica albuginea may originate from the somatic cells of the cortex, either from those of the germinal epithelium, or from those which, because of their position, may be termed prefollicular. There is not apparent ultrastructural confirmation of an active steroidogenic capacity in the albugineal cells. A possible physiological difference between clear cells and dark cells due to a different electron density, is furthermore discussed.

Animals