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L Mezenský

Publications and source records attributed to L Mezenský.

7 recordsLinked to original sources
PubMed1982

[The epizootiological significance of positive bacteriological findings on Mycobacterium tuberculosis and Mycobacterium bovis in humans].

The relation between the active form of tuberculosis in persons working in agriculture and incidence of tuberculosis in cattle was analyzed in 1974 to 1978, i.e. in the period after the elimination of bovine tuberculosis in Czechoslovakia (in 1968). M. tuberculosis was isolated in 15 cases and M. bovis in four cases of persons employed by the farms on which the Regional Hygienic Station, Brno, was responsible for the microbiological diagnostics of tuberculosis. Direct contact with animals was demonstrated in eight patients; M. tuberculosis was isolated from seven of these patients and M. bovis from one. Seven cattle herds were exposed to spontaneous infection by M. tuberculosis and in one of them tuberculosis was not demonstrated during complex examination. In three herds the examination revealed only a sensitivity of cattle to mammalian tuberculin. In other three herds tuberculosis was detected by allergic tests, patho-anatomic examination and bacteriological examination. M. tuberculosis in cattle was detected in two herds. The occurrence of bovine tuberculosis caused by a cattle tender with a positive finding of M. bovis in sputum was demonstrated in one herd. Virulence for the tested cattle was found in one strain (isolated from a mesenterial lymph node of cattle) of the four strains of M. tuberculosis used for the experimental infection of 17 animals. On the other hand, in three strains of M. tuberculosis, trials with experimental infection demonstrated only allergy to mammalian tuberculin and changes at the sites of subcutaneous inoculation of mycobacteria of regressive nature; these mostly disappeared within 90 days from infection.

Agricultural Workers' Diseases↗
PubMed1982

Taxonomic analysis of some slowly growing atypical mycobacteria.

A taxonomic analysis of 92 strains of slowly growing mycobacteria received as members of 11 species of the genus Mycobacterium showed that the 45 characters used allowed to reliably recognize M. avium-intracellulare, M. xenopi, M. kansasii, M. shimoidei, M. terrae, M. nonchromogenicum and M. triviale as distinct species. A numerical taxonomic method revealed that strains of these species formed distinct clusters, which was confirmed by calculated mean values of intra- and intertaxon similarity. A close relatedness was found between M. scrofulaceum and M. avium-intracellulare species which could not be well differentiated from one another by the tests used. A small number of M. novum strains in the study did not allow a clearcut distinction between this taxon and similar strains of M. nonchromogenicum and M. terrae species. Both collection strains of M. gastri differed considerably from one another: one exhibited a certain relatedness to M. kansasii taxon, the other one to M. avium-intracellulare. This study allowed to prepare a table which presents the frequencies (%) of characters positivity for each of the taxa studied. The table is intended as a diagnostic tool in routine identification procedures conducted in field mycobacteriological laboratories.

Mycobacterium↗
PubMed1977

Comparison of microscopic positivity in smears from sputa stained according to Ziehl-Neelsen in different modifications.

Some basic factors influencing the detection of microscopic positivity in the sputa of patients with tuberculosis of the lungs, stained according to Ziehl-Neelsen in various modifications, were studied in 9 laboratories in 5 different countries (Bulgaria, Czechoslovakia, Mongolia, German Democratic Republic and Poland). Each laboratory prepared 200 coded slides from 50 suspect-positive sputa. The slides were stained using four different methods and despatched, without examination, to the next laboratory in the series. A total of 1 800 slides from 450 sputa were thus evaluated and each smear was successively examined by 8 different readers. The study has shown that the result of direct microscopy depends primarily on the individual qualities and attentiveness of the workers evaluating the slides while the remaining factors are less important. Mechanical homogenization of sputa before making the smear, carried out by shaking the sputum with glass beads, had a significant effect on the number of detected mycobacteria while homogenization using soda lye did not influence the positivity in any direction. The detection of mycobacteria after hot staining was significantly higher than after a 2-hour action of carbolfuchsin without heating. Additional staining with picric acid or malachite green had no effect on the amount of bacteria detected and the method of examination (in one line or meander-like on selected spots) was found to be completely unimportant.

Humans↗