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Biomedical subjects

L N Bubnova

Publications and source records attributed to L N Bubnova.

At least 19 recordsLinked to original sources
PubMed2005

[Clinical significance of lymphocyte subpopulations and cytokine-producing activity of blood cells in patients with primary myelodysplastic syndrome].

Lymphocyte subpopulation composition and spontaneous production of tumor necrosis factor-alpha (TNF-alpha) by blood cells were studied in 22 and 15 patients with myelodysplastic syndrome (MDS), respectively, who were under immunosuppressive therapy (IST). Patients with hematological response revealed a significantly increased cytotoxic CD8+ lymphocyte concentration and TNF-alpha production. A direct correlation between TNF-alpha production rate and CD8+ lymphocyte level was established. Maximum cytokine-producing activity of blood cells was identified in MDS patients with enhanced erythroid response. The study established a great diversity of MDS, its hypoplastic variants and the prognostic value of spontaneous production of tumor necrosis factor-alpha (TNF-alpha) by blood cells as marker of IST efficacy.

Adult↗
PubMed1999

[Immunomodulator Cycloferon in the comprehensive treatment of patients with acute non-lymphoblastic leukemia].

The therapeutic benefit and action of the immunomodulator Cycloferon were evaluated in patients with acute non-lymphoblastic leukemia. When administered in conjunction with chemotherapy during first complete remission, it reduced both early-onset relapse frequency and degree of cytostatic-induced immune deficiency. The drug's action is mediated by changes taking place in cytokine and interferon synthesis.

Acridines↗
PubMed1998

[Prognostic value of some biochemical and immunologic factors in the management of multiple myeloma].

The results of examination of 58 patients in the course of multiple myeloma (MM) are discussed. Prognosis for LDH, beta2-m and FNO-alpha administration was evaluated versus immunochemical pattern, tumor mass and response to different chemotherapeutic regimens. High prognostic value of serum levels of LDH, beta2 and FNO-alpha in MM patients was observed. The method may be useful in carrying out chemotherapy and detecting recurrences and chemoresistance.

Adult↗
PubMed1997

[The HLA phenotype and duodenal ulcers in patients following kidney transplantation].

The authors have performed an investigation of the association between ulcer disease of the duodenum and bleedings from the ulcers in 204 patients after transplantations of the kidney with the antigen of HLA system histocompatibility. Duodenal ulcers were found in 25 patients (12.9%), in 11 of them the ulcers were complicated by bleedings which was 5.4% of the total number of the patients with the transplanted kidney. The evaluation of the HLA antigen distribution has revealed an association of duodenal ulcers with A2 and B13 antigens which took place significantly more often than in a group of practically healthy people (RR = 1.85 and 1.9 correspondingly). In patients with the transplanted kidney and following ulcerous bleedings this association was more obvious (RR = 2.32 and 3.74 correspondingly). According to the calculated etiological fraction 40% of the patients can be supposed to have the etiological association of the disease with A2 antigen and 20% of the patients with B13 antigen. A typical feature was the presence of possible "protective" A9 antigen which was found in the group under study two times more seldom than in the control group (RR = 0.25). A comparison of the distribution of HLA antigens in the patients in whom there developed bleeding duodenal ulcers with those who had no such complications has also established the association of the disease with HLA A2 and B13 antigens. The probable "protective" A9 antigen was also found. Since the kidney transplantation is preceded by determination of the recipient's HLA phenotype, these data can be used as prognostic criteria as well as the indications to performing preventive antiulcerous therapy in patients with high risk of the development of ulcerous lesions of the gastrointestinal tract.

Adult↗
PubMed1996

[Immunologic monitoring and immune reconstitution in recipients of allogeneic bone marrow].

The dynamics of different peripheral blood lymphocyte subpopulations (CD3+, CD4+, CD8+, CD22+, DR+, CD25+ and CD16+) and levels of serum cytokines TNFa and IL1 beta were evaluated in 10 patients with allogeneic bone marrow transplantation (BMT). It was established that initially low levels of practically all the lymphoid subsets studied came back to normal in patients with favorable post-BMT course, while, in unfavorable post-BMT course (GVHD or relapse), the levels of said lymphoid subsets remained unchanged or even dropped. Post-BMT increase in serum TN alpha level can be of prognostic value for GVHD development whereas serum IL1 beta level does not correlate with post-BMT course.

Bone Marrow Transplantation↗
PubMed1995

[A granulopoiesis inhibitor. Synthesis and biological activity].

Three schemes of synthesis for pentapeptide Glp-Glu-Asp-Cys-Lys-OH were compared was carried out. Acetamidomethyl protection was used for the mercapto group of cysteine. For the same purpose, cystine was used as the starting compound for synthesis. The optimal method was shown to be the solid phase method with S-acetamidomethyl cysteine protection that can be removed by mercuric acetate before the cleavage of a peptide from a polymer. The stabilized peptide inhibits proliferation of bone marrow cells of patients with chronic myeloleukemia 5- to 20-fold and has a less pronounced effect (up to 2-fold inhibition) on peripheral blood cells. Thus, its application for the therapy of hemoblastoses is promising.

Amino Acid Sequence↗
PubMed1995

[An analysis of DNA reparative synthesis in the lymphocytes of bronchial asthma patients].

Spontaneous and UV-stimulated unschedule syntheses (US) of DNA in the peripheral blood lymphocytes and HLA antigens were investigated for asthmatic patients. The index of stimulation (IS) of DNA repair was determined as the ratio of the values of UV-stimulated US to spontaneous US. In the patients, a significant elevation of the intensity of spontaneous DNA US and the decrease in IS values were revealed as compared with the healthy controls. At the same time the intensity of UV-stimulated IS did not differ in patients and the control subjects. The values of IS appeared to be significantly higher in those patients who had the HLA-Al antigen in their phenotype compared to those who had not. Possible mechanisms for the alteration of the DNA repair capacity of lymphocytes and for the relationship between cytogenetic damages and immunogenetic features in asthmatic patients are discussed.

Adult↗
PubMed1995

[Morphological and pathogenetic aspects of aplastic anemia].

A complex study of colony-stimulating properties of cells-precursors of granulomonocytopoiesis, morphological parameters of the marrow histological structures and immunological parameters was carried out in 90 patients to specify pathogenic mechanisms of aplastic anemia (AA) development. 95% of patients appeared to have sharp decrease of the marrow colony-stimulating properties and only 5% had normal initial indexes. In most patients endostal cell number increased, while the content of reticulocytes grew higher in more than 30% of patients. Defect of the marrow stroma functioning was registered in 23% of patients. Increase of the osseous tissue volume and osteocyte number was found. Dysbalance in cellular link of immunity was demonstrated. Only temporal increase of the marrow colony-stimulating properties was marked in some patients on the background of treatment with antilymphocyte globulin, despite the clinic-hematological improvement. Multifactor pathophysiological mechanisms, the main of which is the stem cell defect lies in the base of AA pathogenesis. Hemopoietic microenvironment pathology is not the leading reason of the hemopoiesis depression but it contributes greatly in its development. Immunological alterations in patients with AA are secondary and can be partly corrected with treatment with ALG.

Adolescent↗
PubMed1994

[Distribution of alleles of DRB, DQB and DQA loci of major histocompatibility complex in healthy donors of Saint Peterburg].

The distribution of alleles of DRB, DQB and DQA loci was investigated in 118 healthy donors from St. Petersburg. Said alleles were identified by the restriction fragment length polymorphism method using DRB, DQB and DQA probes after TaqI digestion and Southern blotting. The frequencies of said alleles were compared with those of a cohort of healthy donors living in Germany. A significantly higher frequency of DRB-17-2 was identified in the Russian donors, as compared with the German counterparts. Also, the Russian donors revealed a decrease in the frequency of DQB-1 (P = 0.01) and an increase in that of DQB-X allele (P = 0.05). An analysis of DRB, DQB and DQA locus a alleles adhesion in a sample of donors from St. Petersburg showed it to agree, in a large proportion of cases, with the literature data. A parallel study of immunological specificity of DR antigen was undertaken to compare the results of genetic and serologic typing; errors in DR specificity identification were found to have been made in 17.0%.

Alleles↗
PubMed1993

HLA antigens in borderline and essential hypertension.

The subject of investigation was the comparison of HLA antigen distribution in people with borderline hypertension (BH) and essential hypertension (EH). One hundred twenty-one men with BH and 60 men with EH were studied. The HLA frequency was compared with that in 858 healthy men, who were blood donors. In BH the increase of B16, B18, and Cw4 and the decrease of A11 and B5 antigen frequency were found. In comparison with St. Petersburg's healthy population, patients with EH were characterized by increasing antigens B18, B41, Cw2, and Cw4 frequency. The coincidence of increased antigen B18 and Cw4 representation in BH and EH allows us to consider them to be immunogenetic markers or predictors of development of EH.

Adolescent↗
PubMed1993

[The characteristics of CD18, CD11b, CD4, CD8 and CD3 antigen expression detectable by ICO monoclonal antibodies in the blood of hemoblastosis patients].

CD18-positive cells (beta-chain of antigen LFA-1), cells CD11b+, CD4+, CD8+ and CD3+ were quantified in the blood of 32 acute leukemia patients, 38 chronic lymphoid leukemia patients and 15 mature-cell lymphosarcoma patients. A standard reaction of indirect immunofluorescence was employed using ICO monoclonal antibodies. Alterations in the content and in the proportions of the main effector populations in the peripheral blood of hemoblastosis patients were observed. These were more apparent in the unfavourable course of acute leukemia and in chronic lymphoid leukemia. The defect was more marked in CD18+ and CD11b+ cells expressing antigen LFA-I. Reduced expression of LFA-I considerably contributes to impairment of antitumor resistance in hemoblastosis.

Acute Disease↗
PubMed1991

[Tissue typing using a Soviet automated luminescent microscope-photometer].

Automated fluorescent microscope developed by the authors permits photometry of microquantities of cellular suspensions in scanning standard 60-well plates with a flat bottom. Automated and semiautomated modes of operation are possible. Fluorescent stains and schemes of staining the examined cells that yield stable results in the lymphocytotoxic test have been selected. Comparative analysis of the efficacies of histocompatibility antigens detection by the routine and the fluorescent technique has shown a number of advantages of the fluorescent method. Specific features of fluorescent stains used for staining live cells are described.

Histocompatibility Testing↗