Four IgG1 monoclonal antibodies (MoAbs) to the Rh antigen D, produced by stable Epstein-Barr virus transformed B-lymphoblastoid cell lines were assessed in different serological tests. Their suitability in blood group typing was shown in antiglobulin, enzyme or albumin methods. The specificity and activity of MoAbs was tested with a panel of red cells of various Rh-phenotypes. The supernatants of all four lines showed anti-D specificity and ability to react with Du red cells. Mean level of MoAbs concentration was near 10 micrograms/ml. These human anti-D MoAbs proved to be useful D-typing diagnostic reagents.

Agglutination Tests↗

PubMedDate not supplied

[Monoclonal antibodies with anti-N specificity].

In this article serologic characteristics of monoclonal antibodies with anti-N specificity is given. Antibodies are directed to homologous sequence of N-form of A glycophorine (group-specific N-antigen) and B glycophorine, expressed both on N- and on M-erythrocytes. Higher titre and avidity of monoclonal antibodies with anti-N specificity in relation to erythrocytes with N-phenotype make it possible to detect N-antigen in material subjected to expert evaluation.

Agglutination Tests↗

PubMedDate not supplied

[Monoclonal antibodies to the human group-specific antigen].

Serologic description of murine monoclonal antibodies (H-86/44 and H-86/50) which are capable to detect a membrane-connected form of human H antigen is given. The antibodies are notable for ability (H-86/44) to be absorbed by salivary H substance of ABH secretors i.e. to detect a soluble form of H antigen. Highly specific, highly active and standard anti-H reagents may be produced on the basis of the anti-H monoclonal antibodies obtained.

ABO Blood-Group System↗

PubMedDate not supplied

[Monoclonal anti-D immunoglobulin for prevention of hemolytic disease of the newborn].

Stable human-mouse heterohybridoma producing human monoclonal anti-D IgG1 has been derived by fusing Epstein-Barr virus (EBV) transformed human immune lymphocytes with mouse myeloma. The absence of EBV DNA in heterohybridoma was demonstrated by the polymerase chain reaction. The antibody was purified by affinity chromatography on protein A Sepharose and tested for sterility, pyrogenicity and toxicity according to the Pharmacopoeia XI and the ability to clear from circulation RhoD-positive red cells. The time of clearance of sensitized RBC was about 3 hrs. Since it is believed to be an association between the rate of RBC clearance by anti-D and the ability of the antibody to suppress the immunization, the monoclonal immunoglobulin may prove suitable for immunoprophylaxis of RhD hemolytic disease.

Animals↗