Paternity blood grouping tests using legally unacceptable testing systems.
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Blood Group Antigens↗
Biomedical subjects
L N Sussman
Publications and source records attributed to L N Sussman.
The Kidd minus-minus phenotype in a Caucasian.
This is the reported example in a Caucasian with the phenotype Jk(a-b-). A strongly reacting antibody was present which was of a single specificity and which could be completely absorbed by either Jk(a+b-) or Jk(a-b+) cells. Since no compatible donors could be found, autotransfusion was done. Family study failed to reveal any other members with the Jk(a-b-) phenotype. The question of a third allele at the Kidd locus is considered.
Blood Group Antigens↗
Harvesting of granulocytes using a hydroxyethyl starch solution.
The need for sophisticated ocmponent therapy has resulted in improved techniques for obtaining concentrates of platelets and granulocytes. The use of single donors as a source for these products is advisable to avoid multiple sensitizations. Obtaining concentrated granulocytes represents a problem because of the difficulty in separating granulocytes from red blood cells by differential centrifugation or sedimentation since the specific gravities are similar. Hydroxyethyl starch (HES) makes the separation more effective. A solution made of 250 ml of 6 per cent HES, 250 ml of distilled water, and 15 g of sodium citrate in 30 ml distilled water provided a satisfactory anticoagulant solution for this purpose. The granulocytes collected averaged 49 per cent of the total available in the processed blood; the platelets averages 82 per cent. A satisfactory yield could thus be obtained from a single donor, and this could be repeated several times in a month. The value of ABO, Rh, and HL-A compatibility between donor and recipient probably increases the viability and safety of this procedure. The Haemonetic No. 30 Cell Separator provided an easy and rapid method for this procedure.
Blood Cell Count↗
Platelet and granulocyte pheresis.
The need for sophisticated component therapy has resulted in improved techniques for obtaining concentrates of platelets and granulocytes. The use of single donors as a source for these products is advisable to avoid multiple sensitizations. A method for obtaining large quantities of platelets has been established rather easily. Obtaining concentrated granulocytes represents a greater problem owing to the difficulty in separating granulocytes from red cells by differential centrifugation or sedimentation since the specific gravities are similar. Separation is easier by the use of hydroxyethyl starch. A modified solution of hydroxyethyl starch and sodium citrate was used with good results.
Blood Platelets↗