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Biomedical subjects

L Nebel

Publications and source records attributed to L Nebel.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

The effect of alpha-fetoprotein on the growth of placental cells in vitro.

Cell cultures of early mouse placentae were studied in the presence of mouse amniotic fluid or isolated alpha-fetoprotein (AFP). Four cell types were observed: polygonal cells, giant cells, small round cells and fibroblasts. The number of polygonal cells increased in placental cultures in the presence of amniotic fluid or AFP, suggesting de novo formation and proliferation in vitro. These preparations were found to stimulate the DNA synthesis in polygonal cells, as demonstrated by 3H-thymidine labelling experiments. Polygonal cells also show a significant increase in 3H-leucine incorporation, indicating more active protein synthesis under the effect of amniotic fluid or AFP. These data suggest that AFP may be one of the fetal factors promoting trophoblastic differentiation.

Amniotic Fluid↗
PubMedDate not supplied

Survey of carnitine content of human semen using a semiquantitative auxanographic method: decreased semen total carnitine concentration in patients with azoospermia or severe oligozoospermia.

A microbiological method, using the carnitine-requiring yeast, Torulopsis bovina ATCC 26014, was developed to identify samples of human semen which contained low levels (less than 250 micron M) of total carnitine. Of 399 semen samples from a male infertility clinic which were tested, 30 (7.5%) were low in carnitine. Of these, 14 were azoospermic and 16 were severely oligozoospermic. Some azoospermic samples (19 = 58%) and severely oligozoospermic samples (51 = 79%) did not give evidence of low carnitine concentrations. These results indicate that decreased total carnitine concentration in semen occurs in certain classes of azoospermic and severely oligozoospermic patients.

Biological Assay↗
PubMedDate not supplied

Glucose tolerance in pregnant women following treatment for sterility.

The incidence of glucose intolerance in 76 patients with polycystic ovary disease (PCOD) who became pregnant after treatment for ovulation was compared with that in 95 healthy women, matched with respect to age and diabetic background, who conceived without intervention. The incidence of abnormal glucose tolerance test results in the treated group was twice that in the normal group (19.7% vs. 9.5%; P less than .03). Moreover, within the treated group abnormal test results occurred more frequently in patients who became pregnant following induction of ovulation with human menopausal gonadotropin (29%) than in those who conceived as a result of other treatment modalities (12%).

Adult↗