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Biomedical subjects

L Netuschil

Publications and source records attributed to L Netuschil.

46 records · Page 3Linked to original sources
PubMed1989

[Comparison of clinical and microbiological parameters for determination of actual caries risk].

In 28 children and adolescents, age 4-17, the numbers of decayed tooth surfaces, filled surfaces and the extent of plaque coverage measured with a plaque scoring index were recorded as clinically assessible caries risk factors. Subsequently paraffin stimulated saliva samples were taken from the same subjects and evaluated microbiologically for their content of caries related microorganisms (S. mutans, lactobacilli). Statistical analysis of correlation revealed only weak, mostly not significant correlations between clinical and microbiological findings. The results are discussed.

Adolescent↗
PubMed1989

[Comparison of flow rate of sulcus fluid after 30 seconds and 3 minutes test times].

Within the framework of a gingivitis study the sulcus fluid flow rate (SFFR) at the lower and upper premolars of 9 subjects was determined with the assistance of Periotron 6000. The measurements were conducted at days 0, 7, 14 and 21 with the aim to compare the SFFR after 3 minutes (Löe and Holm-Pedersen 1965, Rüdin et al. 1970) with the measurements of Lamster et al. (1985a, b) at 30 seconds. The increase of the SFFR at 30 seconds, as well as at 3 min was significant on all measurement days, but no proportional dependance of SFFR on the measurement time was seen. On the basis of these results it is justified to restrict the SFFR to 30 seconds, to reliably determine the grade of infection of gingiva.

Gingival Crevicular Fluid↗
PubMed1980

Antibody-mediated polysome precipitation as a method for the size determination of viral mRNA species: viral envelope glycoprotein mRNA of avian sarcoma viruses.

A method was developed that allows the in situ isolation of viral mRNA, i.e. from polysomes of infected cells. This was achieved by precipitating intact polysomes via their nascent virus polypeptides using virus-specific antibodies. As a model, antibodies to the major envelope glycoprotein (gp85) of avian sarcoma viruses were employed to precipitate those polysomes from infected cells which synthesized the corresponding p70 virus glycoprotein precursor. Normal immunoglobulin and polysomes from uninfected chicken embryo fibroblasts served as controls. The radioactivity labelled mRNA from antibody-precipitated polysomes could subsequently be extracted and characterized for size. It was found that avian sarcoma virus gp85 envelope glycoprotein is predominantly synthesized by a 22--28 s viral mRNA. In addition, minor amounts of gp85-specific mRNAs of 16--21 s and 28--35 s could be demonstrated. The data indicate the presence in polysomes of viral mRNA species coding for i) gp85 only (16--21 s RNA), ii) gp85 and pp60src protein from the adjacent src-gene (22--28 s RNA), and iii) a large viral precursor protein (28--35 s RNA).

Animals↗
PubMedDate not supplied

A modification of the cell culture agar diffusion test using fluoresceindiacetate staining.

In order to reduce the uncertainties involved in the morphologic evaluation of the cell culture agar diffusion test, the originally recommended neutral red stain was replaced by fluoresceindiacetate (FDA). This stain proved to be nontoxic in the concentrations used in this study (0.002%). Toxicity tests with phenol, formalin, and methylmethacrylate monomer revealed results corresponding to literature evaluations by other methods. Since FDA makes cell metabolism visible and easily accessible to automated evaluation techniques, it presents certain advantages over the morphologic evaluation using the neutral red stain.

Agar↗