PubMed Health⌕ Search

Biomedical subjects

L Odum

Publications and source records attributed to L Odum.

50 records · Page 3Linked to original sources
PubMed1987

Protein I serotype of serum-resistant versus serum-sensitive Neisseria gonorrhoeae strains.

In order to characterize serum-resistant and serum-sensitive strains of N. gonorrhoeae, the protein I serotype, auxotype, and penicillin susceptibility of 128 strains were tested. Sensitivity to the complement-dependent bactericidal activity of normal human serum was highly associated with protein I serotype (p less than 0.001). Thus 85% of serotype 1-3 strains were serum-resistant, whereas 86% of serotype 8 strains and all strains with serotypes 8 + 9 or 9 were serum-sensitive. Serum-resistance or sensitivity for a given serotype was independent of auxotype. The susceptibility to penicillin within the serotypes 1-3 was significantly associated with auxotype (p = 0.0016); all AHU- (requirement for arginine, hypoxanthine and uracil) strains had MICs of penicillin of 0.04 microgram/ml or less and were serotypes 1-3. Among the non-AHU-strains, serotype 9 was significantly more penicillin susceptible than the other serotypes (p less than 0.003).

Blood Bactericidal Activity↗
PubMed1987

Indirect haemagglutination test using gonococcal pilus antigen: how useful to diagnose gonorrhoea?

In 1979 an indirect haemagglutination test (gonococcal antibody test) using gonococcal pilus antigen replaced the gonococcal complement fixation test as our routine procedure to show gonococcal antibodies. In the diagnosis of current gonorrhoea the sensitivity of the gonococcal antibody test was far superior to that of the gonococcal complement fixation test (about 55% versus 9% for first episode gonorrhoea). To evaluate the usefulness of the test result the following population groups were studied: 1376 patients undergoing medical examination for gonorrhoea (386 had gonorrhoea), 1384 healthy people aged 15-65, 54 patients with meningococcal disease, 30 children with respiratory tract infection, and 254 patients with evidence of various diseases other than neisserial infections that might be associated with symptoms of arthritis. These investigations showed that (1) non-specific positive gonococcal antibody test results occur rarely, (2) at least half the people who have had gonorrhoea remain seropositive (with titres of 1/40 to 1/160), and (3) a positive test result is more significant the younger the patient and the higher the titre. For younger people a positive test result should always be followed up by bacteriological examination; in all age groups titres of 1/320 or more should indicate medical examination for current gonorrhoea.

Adolescent↗
PubMed1987

Trypsin-inhibitory activity of ovalbumin preparations is due to ovomucoid.

Based on the amino-acid sequence it has been proposed that ovalbumin is a proteinase inhibitor. The inhibitory activity against trypsin, chymotrypsin, pancreatic elastase, plasmin, thrombin and pancreatic kallikrein was tested, but none of these proteinases were inhibited by ovalbumin. A commercial preparation of ovalbumin contained an impurity with trypsin inhibitory activity. This impurity was probably ovomucoid.

Animals↗
PubMed1986

Human inter-alpha trypsin inhibitor and immunologically related inhibitors investigated by quantitative immunoelectrophoresis. I. Method and reference material.

The concentration in serum of inter-alpha trypsin inhibitor (I alpha I) and its immunologically related inhibitor, the prealbumin-like proteinase inhibitor (pA-PI), was investigated by quantitative crossed immunoelectrophoresis (CIE) in 33 healthy persons. The corresponding urinary excretion over a 4 h period of the immunologically related urinary proteinase inhibitor (UPI) was measured by rocket immunoelectrophoresis. The concentration of pA-PI increased significantly (p less than 0.001) with age whereas that of I alpha I and the urinary excretion of UPI were independent of age. There was no significantly difference in the concentration of the inhibitors in serum between the sexes, but females were found to have a lower urinary excretion of UPI than males (p less than 0.05). Furthermore we found no significant variation in the concentration of the inhibitors during the course of the day or from day to day over a 5 day period. Results obtained by rocket immunoelectrophoresis correlated well with enzymatically measured trypsin inhibitory activity.

Adult↗
PubMed1984

Endocarditis due to Kingella kingae.

Four cases of endocarditis due to Kingella kingae are described in compromised patients. All had primary heart disease, and two had systemic lupus erythematosis and congenital heart defect respectively, in addition. Confirmation of Kingella kingae was made in one case at autopsy. The literature on 11 cases of endocarditis, 2 bacteremia, 4 osteomyelitis, 5 septic arthritis and 1 intervertebral disc infection, all caused by Kingella kingae, is reviewed. Our findings confirm that the organism is of low pathogenicity. Children may be predisposed to infection with Kingella kingae.

Adolescent↗
PubMed1984

Slow migrating proteinase inhibitors in human urine.

By means of a sensitive electrophoretic technique for the detection of proteinase inhibitors three slowly migrating proteinase inhibitors (SMPI) were discovered in some samples of pathological urine. SMPI 1 migrated in the beta 2-zone whereas SMPI 2 and SMPI 3 appeared in the anodal and cathodal gamma-zone, respectively. Only SMPI 1 and 2 were examined in detail. These were found to inhibit tryptic and elastolytic digestion, but not chymotryptic or plasminolytic digestion of casein. Immunological investigations revealed no similarity to normally occurring proteinase inhibitors in serum and urine. The SMPIs from one sample of urine were partially purified by DEAE-Sephadex ion exchange chromatography, followed by gel filtration on Sephacryl superfine 200. This procedure did not separate the two inhibitors. The molecular masses were estimated to be 25 000 Da by gel filtration, and 23000-26500 Da by SDS polyacrylamide gel electrophoresis.

Chromatography, Gel↗
PubMed1984

Slow migrating proteinase inhibitors in human serum.

Employing a sensitive semi-quantitative electrophoretic-technique on acid-deproteinized serum, we found two previously unrecognized trypsin inhibitors migrating as beta 2- and gamma-globulins, respectively. The two inhibitory bands were also detected in native serum. They were not seen in 3 healthy persons, but were found in patients with uremia, cancer, inflammatory diseases and collagenosis. Immunological investigations showed no cross-reaction with antibodies against seven well-known proteinase inhibitors. The two trypsin inhibitors also inhibited pancreatic elastase.

Antibodies↗
PubMed1983

Electrophoretic investigations of acid-stable proteinase-inhibitory activity in human serum.

By means of a sensitive semiquantitative electrophoretic technique involving tryptic digestion of immobilized casein embedded in agarose gel, the acid-stable proteinase-inhibitory fraction of human serum was shown to consist of two fractions with alpha 1- and prealbumin mobility, respectively. The prealbumin-like migrating inhibitor (pA-PI) was previously thought to be the only inhibitor present in the acid supernatant. The pA-PI was demonstrated in native serum as well, but was not recovered quantitatively upon acid precipitation. Using antibody-containing intermediary gels pA-PI was shown to react with inter-alpha-trypsin inhibitor antibodies, whereas the alpha 1-inhibitor reacted with alpha 1-proteinase inhibitor antibodies. The pA-PI activity was compared to serum levels of creatinine and C-reactive protein using a limited number of patients. The inhibitor level was clearly increased in patients with high serum creatinine and in some cases of high concentrations of C-reactive protein in the serum. Only small amounts of pA-PI were detected in sera of healthy people.

Caseins↗
PubMed1982

Reliability of the plaque assay to determine the sensitivity of Neisseria gonorrhoeae to the bactericidal activity of normal human serum.

A plaque assay for determination of the sensitivity of Neisseria gonorrhoeae to the complement-dependent bactericidal activity of normal human serum was evaluated. The method was found to be rapid and easy to perform. All strains, which gave a positive result by the plaque assay, were serum-sensitive in a quantitative assay, whereas only 78% of strains giving negative results by the plaque assay were true serum-resistant. The growth of a few gonococcal strains was inhibited, i.e. formed plaques, after exposure to genuine as well as to heat-inactivated normal human serum.

Bacteriological Techniques↗
PubMed1982

Studies on Neisseria gonorrhoeae strains from test-of-cure specimens. Correlation between the in vitro susceptibility to penicillin and the sensitivity to the complement-dependent bactericidal activity of normal and convalescent human serum.

Sixty-seven out of 88 Neisseria gonorrhoeae strains isolated from test-of-cure (TOC) specimens during a five-months' period were included in the study. For 62 patients sufficient information was obtained in order to distinguish between relapse (34 ptt) and re-infection (28 ptt). For comparison with strains from these two groups of patients, 63 urogenital and 21 pharyngeal gonococcal strains isolated during the same period of time were randomly selected. The distributions according to susceptibility to penicillin for TOC strains and control strains corresponded to those found for the total number of TOC strains (275) and other strains (3,345) tested in 1979, respectively. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent bactericidal activity of normal human serum. However, gonococcal strains less susceptible to penicillin in vitro (MIC values within the range 0.1-2.0 microgram/ml) were significantly more sensitive to the complement-dependent activity of normal human serum (P less than 0.01) than strains fully susceptible to penicillin (MIC less than 0.01 microgram/ml.) Penicillin-resistant strains (MIC greater than 2.0 microgram/ml) did not differ from strains susceptible to less than 0.1 microgram penicillin/ml and were slightly more serum-resistant than the less susceptible strains (P less than 0.05). No difference in serum-sensitivity of urogenital and pharyngeal isolates could be demonstrated. The level of bactericidal activity of homologous convalescent serum was unrelated to the presence of antibodies either to gonococcal pili or crude gonococcal antigen preparations. The sensitivity to normal human serum of a certain strain was not correlated with sensitivity to homologous convalescent serum.

Blood Bactericidal Activity↗
PubMed1981

Identification and characterization of Kingella kingae.

Kingella kingae is a rarely isolated opportunistic pathogen in the family Neisseriaceae. Thirteen strains of this organism, including six strains isolated in Denmark, were characterized. They formed a homogeneous group of small, non-motile, fastidious Gram-negative rods which were beta-haemolytic, oxidase positive, catalase negative and saccharolytic. Acid was produced for glucose and maltose. Growth was most pronounced aerobically but corroding colonies increased considerably in size during anaerobic incubation.

Anti-Bacterial Agents↗