Biomedical subjects

L Ostblom

Publications and source records attributed to L Ostblom.

3 recordsLinked to original sources

PubMed1991

Bone biopsy in the horse. 2. Evaluation of histomorphometric examination.

The histomorphometrical examination of a series of bone biopsies from the wing of ilium of foals and older horses is reported. Biopsies were obtained from 20 clinically normal horses, which had previously been infused with tetracycline intravenously twice at a 7 day interval at a dose rate of 10 to 15 mg kg-1. The horses were divided into 2 groups. Group 1 consisted of 10 foals (mean age: 4 +/- 1 months) which were biopsied at 3 different sites in the wing of ilium. Group 2 comprised 10 older horses (mean age: 91 +/- 63 months) which were biopsied from only one site in the left ilium. A systematic evaluation of intra-observer, intra-core, intra-bone and inter-bone errors was made to investigate parameter variations that might affect interpretation of bone biopsies. The measurements carried out included cancellous bone volume (Cn-BV/TV), osteoid surface (OS/BS), eroded surface (ES/BS), mean wall thickness (W.Th), mean osteoid thickness (O.Th), distance between labels (Ir.L.Th) and mineralizing surface (MS/BS). The intra-observer variance, the variance between sections, the variance between adjacent biopsies and that between right and left sides proved to be smaller than the inter-individual variance. The estimate of errors associated with the histomorphometrical analysis of bone biopsies is important for correct interpretations of changes due to disease.

Animals↗

PubMed1989

Navicular bone disease: a comparative histomorphometric study.

Twelve horses suffering from navicular bone disease were examined in a prospective, controlled histomorphometric study for six months. The objective was to compare the histology of navicular bones from untreated animals to those treated with the egg-bar shoeing technique. These data were compared to similar sections from three normal animals. The current investigation provided quantitative support to previous findings concerning clinical improvement. Detailed histology, changes in bone morphometry and pathophysiological reactions are discussed.

Animals↗

PubMed1982

Histological study of navicular bone disease.

A histological investigation of navicular bones from 8 horses with navicular disease was carried out to investigate the aetiology of the radiological findings. Two of the horses were double labelled with tetracycline before slaughter in order to assess the vitality of the diseased navicular bone. The examinations revealed no evidence of loss of vitality in the diseased bone areas or in surrounding tissues. A very high rate of remodelling indicated by resorption and formation of bone was present in all cases. This was shown microscopically by the high number of osteoclasts and osteoblasts present, osteoid formation and particularly by a high rate of intravital uptake of tetracycline by the bone. The findings are considered to indicate that navicular disease is not primarily caused by ischaemia and subsequent necrosis, but rather is a consequence of increased activation of bone remodelling caused by altered pressure from the deep flexor tendon on the bone and increased load on the caudal part of the foot. This is further supported by the clinical changes seen after alterations occur in the hoof conformation. The disease is, therefore, considered to be reversible and may be alleviated by altering the load on the navicular bone by special shoeing. Only secondary lesions such as adhesions and spur formation render the disease irreversible.

Animals↗