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L P Anan'eva

Publications and source records attributed to L P Anan'eva.

At least 19 recordsLinked to original sources
PubMed2004

[Designing and clinical testing of immune-enzyme and immunofluorescence test systems for serodiagnosis of ixodes borreliosis].

The methods of immune enzyme assay (MIEA) and of lanthanide immunofluorescence analysis (LIFA) were used to work out the test systems for the detection (in blood serum of patients) of specific IgM IgG antibodies to the B. burgdorferi spirochete--a causative agent of ixodic borrelioses. The test system was clinically tested versus the indirect immunofluorescence reaction (IIFR) and commercial immune enzyme test system (CIET). The results of antibodies' detection were shown to correlate with the analysis data for the same sera in IIFR and to be in line with a real presence or absence of the disease. Test systems based on LIFA were proven to be most sensitive and specific.

Antibodies, Bacterial↗
PubMed2004

[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne borreliosis (Lyme disease). Communication 1. Comparative study of the 1st generation enzyme immunoassay test-systems for detection of antibodies to Borrelia burkdorferi sensu lato].

Four foreign and one Russian 1st generation test-systems for detecting class G antibodies or summary antibodies to Borrelia burkdorferi sensu lato were comparitively investigated with the use of the clinical material under conditions of an encoded experiment. Cross reactions with sera from patients with syphilis, Epstein-Barr infection, cytomegalovirus infection and systemic lupus erythematosus were observed. The best specificity and sensitivity parameters were provided by the Enzygnost Borreliosis test-system.

Animals↗
PubMed2004

[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne Borreliosis (Lyme disease). Communication 2. Comparative study of recombinant enzyme immunoassay test-systems (rELISA) for serological diagnosis of ixode tick-borne Borreliosis].

One foreign and two Russian recombinant enzyme immunoassay test-systems were comparatively investigated under conditions of an encoded experiment. Sensitivity in the experiment with the Russian test-systems Borreliosis-ELISA-IgG and Lyme Best was 63.8 and 68.8% respectively. As for the test-system Borrelia IgG Recombinant, it was 47.5%. All the test-systems were highly specific (94.4 to 99.5%). The test-systems Lyme Best and Borrelia IgG Recombinant revealed partial cross reactions with sera from patients with systemic lupus erythematosus and leptospirosis.

Antibodies, Bacterial↗
PubMed2003

[Comparative analysis of methods for laboratory diagnosis of Lyme's disease in early stages of infection].

Different methods of laboratory diagnostics were comparatively analyzed in examining 25 patients at the early infection stage. Sera were measured by using various serological reactions. Specific antibodies were determined by using the reaction of indirect immune-fluorescence (RIIF), the immune-enzyme analysis (IEA) and the complement-binding reaction (CBR) in 83.1%, 54.4% and 12.5% of cases, respectively. Essential differences in sensitivity were detected between the above methods. RIIF was proven to be a reliable and sufficiently sensitive method in the laboratory diagnostics of Lyma's disease. While the use of two methods, i.e. RIIF and IEA, ensures the highest percentage of detection of antibodies to the causative agent of Lyma's disease.

Adolescent↗
PubMed2003

[Comparative analysis of use of two strains of various genotypes of Borrelia burgdorferi sensu lato as antigens for antibody identification in Ixodes tick borreliosis by indirect immunofluorescence].

The results of the indirect reaction of immune-fluorescence (IRIF) was studied in testing 49 sera of 19 patients with Lyme-borreliosis with antigens of genotypes Borrelia afzeli (strain Jp-21) and Borrelia burgdorferi sensu stricto (strain No. 17); rabbit fluorescini-sothiocyanate-marked conjugates to human immunoglobulins M and G as well as polyvalent conjugate were used of. No reliable differences were found between all positive and all negative results. The biggest portion of positive results was registered in tests with anti-G-conjugate (up to 92% with strain No 17).

Animals↗
PubMed2003

[Articular lesions in Ixodes tick-borne borreliosis (Lyme disease)].

Articular lesions in 157 patients infected with ixodes tick-borne borreliosis (ITB) in a central Russia's region set on, on the average, in 4 months after tick attack; they were associated with systemic signs of an early disseminated infection and set on less seldom in a late period. The most often encountered systemic signs were as follows: secondary erythema (32% of patients), neurological syndrome (13%), cardio-vascular lesions (22%), ocular lesions (13%) and hepatic lesions (8%). The articular syndrome manifested itself through arthralgia (53 patients) and arthritis (104 patients), which set on quite often in the tick-attack area. There was a peculiarity typical of articular lesions, which made it possible to distinguish them from other rheumatic disease. A dynamic follow-up revealed different clinical variations of Lyme's arthritis and peculiarities of the genetic profile, i.e. a higher prevalence of HLA A2, HLA-B15 and HLA-DR4 as well as of haplo-types HLA A2-B15 and HLAB15-DR4. The articular lesions were associated with an intensive specific humoral immune response. The instrumental examination methods, i.e. ultrasonography of joints as well as scintigraphy of bones and joints, did not reveal any qualitative differences between arthralgia and arthritis, which is indicative of a common nature different-intensity manifestations of arthropathy in thick-borne borreliosis.

Adult↗
PubMed2002

[Detection of anti-Borrelia antibodies by immunoblotting in Lyme borreliosis].

IgM and IgG were detected in the sera from 25 Russian patients with Lyme disease by immunoblotting test with Immunetics kit (USA). Early stage of the disease was diagnosed in 12 patients and late stage in 13. Specific protein lines were detected in virtually all patients but their number and combination in the sera were different. Eleven (43%) sera were regarded as positive according to the American criteria of seropositive test, 3 of them by IgM and 10 by IgG. In patients with short disease serum reaction was either negative or confined to 1-2 lines per strip. Patients with late stage showed a more manifest extensive reaction, which was the most pronounced in patients with a long lasting disease and articular involvement; the reaction was similar to that characteristic of chronic Lyme arthritis. Parallel testing of IgG antibodies in 25 sera from American patients with Lyme disease (confirmed by direct isolation of the agent) showed notable similarity of reactions of both American and Russian sera. More essential differences were observed only for low molecular weight proteins (p28, p23, p21).

Antibodies, Bacterial↗
PubMed2000

[Age and symptoms of Lyme disease].

AIM: Comparison of clinical symptoms in Lyme disease (LD) in various age groups. MATERIAL AND METHODS: 150 patients with verified LD were divided into 4 age groups: under 15 years (group 1), 16-40 years (group 2), 41-60 years (group 3), over 60 years (group 4). Antibodies to Borrelia burgdorteri were detected with indirect immunofluorescence and Western blot. RESULTS: LD clinical symptoms differed in the age groups. Patients of group 1 had more prevalent infectious syndrome with fever but they had no radiculoneuritis and polyneuritis. Patients of group 2 more frequently suffered of carditis and secondary erythema. Groups 3 and 4 were characterized by infectious syndrome, secondary erythema and aseptic meningitis, joint lesions being more frequent in group 3, nervous system lesions--in group 4. CONCLUSION: Age peculiarities of LD symptoms are very important. In particular, joint syndrome is responsible for lingering course of LD.

Adolescent↗
PubMed1997

[Methods for the instrumental diagnosis and verification of Lyme arthritis].

We used instrumental methods to characterise Lyme arthropathy in 79 patients with joint involvement and history of Lyme borreliosis. All of them had arthralgia and 45 of them also had arthritis. Radiological examination has established subchondral bone sclerosis in sacroiliac joints in 68% and sacroiliitis in 29% of the examinees. Scintigraphy revealed polyarticular lesions in many cases. Ultrasound investigation has found inflammation and edema of periarticular tissues in the knee, shoulders and hips. The level of antibodies against the causative agent Borrelia (in EIA) was significantly higher in patients with marked signs of inflammation in ultrasound examination. Thus, the combined methods of examination in the diagnosis of Lyme arthritis provide the most complete information.

Arthritis, Reactive↗
PubMed1996

[Use of fluoroquinolones in rheumatology].

The efficacy and tolerance of 3 fluoroquinolones (ciprofloxacin, pefloxacin, lomefloxacin) were studied in 157 patients with rheumatic diseases concurrent with infection-induced abnormalities. Infection cure was achieved in 123 (78.6%) patients, as evidenced by clinical, microbiological, and serological studies. The tolerance of fluoroquinolones was regarded as good, satisfactory, and poor in 114 (72.6%), 37 (23.6%), and 6 (3.8%) patients, respectively. All representative drugs of the fluoroquinolones series produced typical adverse reactions. The authors consider it expedient to make further clinical studies to test the optimal treatment regimens and to expand indications for the use of fluoroquinolones in this group of patients.

Adolescent↗
PubMed1995

[The clinical and serological manifestations of Lyme disease in Russia].

Out of 86 Lyme's disease patients with a history of migrating erythema nervous system, cardiovascular and articular involvement was observed in 27, 6 and 43% of cases. Acrodermatitis was diagnosed in 2% of patients. Affection of locomotor system manifested with acute arthritis episodes or pains in major joints. 11 patients of 12 examined at arthritis onset showed elevated titer of anti-Borrelia IgG antibodies. Serologically, of 80 patients with arthritis or arthralgia without prior migrating erythema 6 demonstrated antibodies to 5 and more Borrelia polypeptides.

Adolescent↗
PubMed1995

[The clinical instrumental characteristics of the locomotor involvement in patients who have had Lyme disease].

Locomotor system has been studied in 24 patients with a history of Lyme's disease. All of them had arthralgia, 11 had relapsing arthritis, chronic arthritis occurred in 6 examinees. Arthritis presented as recurrent asymmetric mono-oligoarthritis affecting primarily joints of the lower limbs. Periarticular disorders were detected in 12 patients. Serologically, 18 of 24 patients had elevated titers of antibodies to Borrelia burgdorferi in indirect immunofluorescence test. Scintigraphy revealed polyarticular lesions in many cases, ultrasound investigation of the joints confirmed inflammatory nature of the pathological changes. It is inferred that combined methods of examination in diagnosis of Lyme's arthritis (titers to antibodies to Borrelia burgdorferi, ultrasound investigations, scintigraphy of the joints) provide most complete information.

Adolescent↗
PubMed1995

[The humoral immunological response of patients with Lyme disease].

Indirect immunofluorescence (II), enzyme-labelled antibodies (ELA), immune blotting (IB) were used for serological evaluation of 74 patients with Lyme disease living in the North-West and Central regions of Russia. Both acute and chronic borreliosis can be seropositive or seronegative. As shown by II and ELA, these patients had a moderate humoral response, high titers of the specific antibodies being a rare finding. The range of anti-Borrelia antibodies (ABA) identified by IB is limited and, as a rule, includes antibodies to no more than 5 proteins of Borrelia burgdorferi. The highest ABA levels were associated with locomotor lesions and were significantly greater of the above levels in patients with migrating erythema. Informative value of II and ELA in Lyme's disease diagnosis according to this study is equal because ELA is more sensitive, while II is more specific. They are both of little help in atypical borreliosis presentation. Most available of other adequate serological tests is IB.

Adolescent↗
PubMed1993

[Lyme arthritis].

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Anti-Bacterial Agents↗
PubMed1992

[The results of a clinico-serological examination of 60 patients with Lyme disease].

Clinical and serological examinations were carried out in 60 patients with Lyme disease. The diagnosis was established on the basis of the disease marker--tick-borne migrating erythema and serological data obtained with the use of indirect immunofluorescence and Western blot. The spectrum of clinical manifestations and the rate of different clinical syndromes are described and compared with the data by this country and foreign authors. It is shown that Borrelia infection is capable of imitating rheumatic diseases.

Adolescent↗