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Biomedical subjects

L P Bignold

Publications and source records attributed to L P Bignold.

At least 55 records · Page 3Linked to original sources
PubMed1987

A novel polycarbonate (Nuclepore) membrane demonstrates chemotaxis, unaffected by chemokinesis, of polymorphonuclear leukocytes in the Boyden chamber.

A novel polycarbonate (Nuclepore) membrane having capillary pores of 3 microns diameter and occupying 0.1% of surface area (average minimum spacing of 48 +/- 18.5 microns), a standard polycarbonate filtration membrane (pores 3 microns in diameter occupying 5% of surface area and having average minimum spacing of 3.9 +/- 2.8 microns) as well as a 3 microns pore cellulose acetate filtration membrane, were compared for their abilities to demonstrate chemotactic movement uninfluenced by chemokinetic movement of polymorphonuclear leukocytes in a Boyden-type chemotactic chamber. The chemoattractant used was dilute Escherichia coli culture filtrate employed in a gradient across the membranes to measure chemotaxis and in non-gradient conditions to measure chemokinesis. The new polycarbonate (Nuclepore) membrane provided the clearest demonstration of chemotaxis unaffected by chemokinesis of the polymorphonuclear leukocytes.

Cell Movement↗
PubMed1987

Aortic and coronary embolism of anaplastic small-cell carcinoma of the lung.

A case of macroscopic tumour embolism from an anaplastic small-cell carcinoma of the bronchus to the aortic bifurcation and the left main coronary artery is described. The emboli occluded both vessels and caused sudden death. To our knowledge, this is the first report of coronary embolism by this type of carcinoma. Previous reports of coronary embolism by lung tumours are reviewed briefly.

Aortic Diseases↗
PubMed1987

Amoeboid movement: a review and proposal of a 'membrane ratchet' model.

Diverse cell types, including Amoebae, leukocytes, embryonic cells and tumour cells move about on solid surfaces to accomplish such activities as feeding, bacterial destruction, embryological development and metastasis. Theories of the mechanism of this movement are reviewed and a model is proposed which invokes the existence of specific, laterally mobile, transmembranous structures in the cell membrane, which are reversibly adhesive for both the contractile apparatus of the cell internally, and the substratum externally. By this model, the movement of all these cell types can be explained.

Amoeba↗
PubMed1987

Mechanism of separation of polymorphonuclear leukocytes from whole blood by the one-step Hypaque-Ficoll method.

After centrifugation of heparinised whole blood on Hypaque-Ficoll medium (s.g. 1.114, Ferrante and Thong, 1980) the specific gravities were measured of the layers of medium above and below the band of polymorphonuclear leukocytes (PMN), as well as of the PMN and sedimented erythrocytes. The erythrocytes and PMN were also examined for evidence of shrinkage. The medium above the PMN layer was found to be less dense than the medium below the PMN layer, which in turn was less dense than the original Hypaque-Ficoll medium. Examination of the cells showed that the sedimented erythrocytes and PMN were shrunken and of increased density. The results are consistent with a separation mechanism dependent upon osmotic loss of water from the sedimenting erythrocytes, forming a density gradient in the medium in which the PMN become buoyant.

Cell Separation↗
PubMed1987

Effects of preparative technique on the rate of adoption of crawling-like movements by polymorphonuclear leukocytes.

Various component steps of several "standard" techniques for separating polymorphonuclear leukocytes (PMN) from whole blood were tested for their effects on the rate at which these cells exhibit crawling-like movements when suspended in 100% plasma. The rate was depressed by excessive centrifugation, excessive agitation, erythrocyte lysis techniques, prolonged preparation time and one type of separation medium used. More than 95% of PMN in all preparations excluded the dye Trypan blue. The rate at which PMN exhibit crawling-like movements in plasma could be a sensitive index of the viability of these cells.

Cell Movement↗
PubMed1987

Syphilitic aneurysm of the right subclavian artery presenting with hemoptysis.

A 40-year-old Australian Aboriginal female presented with hemoptysis in association with a right upper zone pulmonary and mediastinal mass. Several syphilitic infections in the patient had been documented during the previous 16 years. Death was due to a large hemoptysis, and autopsy revealed that the mass in the chest was a right subclavian artery aneurysm which had ruptured into the lung parenchyma.

Adult↗
PubMed1986

Crawling-like movements of polymorphonuclear leukocytes in plasma are not a good index of their motility in microporous cellulose acetate membrane.

Crawling-like movements, motility and adhesiveness of polymorphonuclear leukocytes (PMN) in various concentrations of autologous plasma were assessed using microporous cellulose acetate membranes. In 0% plasma, crawling-like movements and motility were low, while adhesiveness was high. In 10% plasma, crawling-like movements remained low, motility was high and adhesiveness fell to a minimum. In 30%, 60% and 100% plasma, crawling-like movements progressively increased, motility rose (at 30%) and then fell slightly (at 100%) while adhesiveness did not change. The results indicate that crawling-like movements of PMN suspended in plasma are not a good indicator of the motility of these cells in cellulose acetate membrane.

Cell Adhesion↗
PubMed1980

Myocardial infarction, papillary muscle dysfunction and mitral valvular incompetence in systemic lupus erythaematosus.

A fatal case of systemic lupus erythaematosus complicated by myocardial infarction, papillary muscle dysfunction and mitral incompetence seven months before death is reported. Necropsy examination of the heart revealed that the infarct was due to multiple occlusive thrombi in epicardial branches of the corresponding coronary artery. No evidence of atherosclerosis or previous coronary arteritis was present.

Adult↗
PubMed1980

Nodular pulmonary amyloidosis associated with benign hypergammaglobulinemic purpura.

A 59-year-old woman, who had had benign hypergammaglobulinemic purpura for three years, developed a solitary amyloid nodule of the lung. The benign hypergammaglobulinemia was associated with an IgG band of restricted heterogeneity and IG2-IgG3 subclass containing complexes of intermediate size with rheumatoid factor activity. Serum levels of IgA and IgM were also increased. Autopsy after death from an unrelated cause revealed no amyloid in the remainder of the lungs or other organs and no evidence of lymphoproliferative disorder.

Amyloidosis↗
PubMed1980

Importance of platelets in increased vascular permeability evoked by experimental haemarthrosis in synovium of the rat.

Increased vascular permeability of synovium induced by experimental haemarthrosis was studied in the stifle joint of the rat. Abnormal permeability was detected by injecting animals intravenously with colloidal carbon and examining the synovial vessels for intramural deposits of carbon. Both fresh and heparinized whole blood injected into the joint induced a marked permeability response of synovial venules which persisted for 18 hours. Platelets suspended in heparinized Tyrode solution induced a similar response, but one lasting only 12 hours. The permeability effects of suspensions of leucocytes and erythrocytes on the synovial vasculature were relatively mild and were maximal 5 and 12 h respectively after intra-articular injection. Heparinized platelet-free plasma had no significant permeability effects. These results indicate that platelets may play an important role in the pathogenesis of synovial inflammation in haemarthrosis.

Animals↗
PubMed1980

Multiple coronary artery aneurysms with aortic sinus aneurysms. A case report.

Aneurysms of the right main, left anterior descending, and left circumflex coronary arteries occurred with aneurysms of all three aortic sinuses in a 44-year-old woman. There was also moderate blood eosinophilia. Necropsy revealed severe chronic inflammation and fibrosis of the arterial and sinus aneurysms, but no evidence of generalized vasculitis.

Adult↗
PubMed1979

Necrotising enterocolitis associated with invasion by Clostridium septicum complicating cyclic neutropaenia.

A fatal case of necrotising enterocolitis complicating cyclic neutropaenia in a 17-year-old boy is reported. The episode of enterocolitis was characterised by fulminant, generalised peritonitis associated with necrosis and gas formation in the wall of the ileum and caecum. Clostridium septicum was grown from premortem blood cultures and Gram positive bacilli typical of this organism were present in histological sections of the bowel wall. Necrotising enterocolitis in this and previously reported cases of cyclic neutropaenia resembles the agranulocytic form of intestinal necrosis which occasionally complicates leukaemia.

Adolescent↗