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Biomedical subjects

L P Lefkovitch

Publications and source records attributed to L P Lefkovitch.

9 recordsLinked to original sources
PubMed1997

Enzymatic responses of Drosophila melanogaster to long- and short-term exposures to ethanol.

The effects of environmental ethanol on larva-to-pupa survival and on the activities of four enzymes were investigated in three Drosophila melanogaster strains. The strains had different allelic combinations at the Odh and Aldox loci on their third chromosomes, but they all carried the Adh(S)-Gpdh(F) allelic combination on the second chromosome. Replicates of each of the strains were exposed to three different ethanol treatments: (i) no ethanol in the medium (control); (ii) 5% ethanol for a single generation (short-term exposure); (iii) 5% ethanol for 20 generations (long-term exposure). In all experiments, the activities of four enzymes (ADH, ODH, GPDH and AOX) were measured in larvae, pupae and adults. The results showed that (i) the larval and adult metabolic responses to environmental ethanol were different; (ii) enzyme activity changes under short-term exposure differed from those measured under long-term exposure; (iii) the activities of the allozymes common to all strains (ADH-S and GPDH-F), differed depending on the genetic background. Changes in larva-to-pupa survival were seen when the larvae of control and exposed lines of the three strains were confronted with various concentrations of ethanol. In all three strains, the exposed lines had significantly higher initial survival rate and ethanol tolerance than the control lines. Strain-specific differences were observed in the ethanol tolerance of both types of line.

Adaptation, Biological↗
PubMed1996

Differences in environmental temperature, ethanol and sucrose associated with enzyme activity and weight changes in Drosophila melanogaster.

Activity changes of three enzymes (ADH, ODH and AOX) of Drosophila melanogaster were followed under different environmental conditions. The influences of ethanol, starvation (no carbohydrates in the medium) and ethanol stress during starvation were studied at both 18 and 26 degrees C. Two strains that were monomorphic for different alleles at the Odh and Aldox loci but otherwise identical were used. The investigated environmental conditions affected ADH induction by exogenous ethanol differently in the two strains. The different allozymes of ODH and AOX also responded differently to the treatments. We observed that the sucrose content of the medium on which ethanol exposure took place and the temperature strongly affected the responses within any single strain. Correlations were estimated among the three enzymes in the larval and adult stages of each strain separately. At both temperatures, differences between strains were observed in the patterns of associations of the response variables, in the larval, but not in the adult stages.

Alcohol Dehydrogenase↗
PubMed1994

The influence of the Odh-Aldox region of the third chromosome on the response of Drosophila melanogaster to environmental alcohol.

Second instar larvae of Drosophila melanogaster were exposed to exogenous alcohol, which is known to influence the activities of several enzymes. In this study, the activity changes were followed in four enzymes (ADH, ODH, alpha GPDH and AOX) during ethanol exposure and compared in three inbred lines that had different allelic combinations at the Odh and Aldox loci. The results indicate that the Odh-Aldox region of the third chromosome may alter the general response to ethanol. The activity of ADH increased considerably in two strains in the larval stages in the presence of alcohol; nevertheless, strain 1, with the OdhS-AldoxF allelic combination, showed a delay in the ADH induction compared to strain 2, which had the OdhF-AldoxS combination. In strain 3 (OdhS*-AldoxS) larvae, ADH induction by environmental ethanol was not detected. Moreover, the activities of alpha GPDH and AOX in strains 2 and 3 were not affected by ethanol. In contrast, the activities of all four enzymes in strain 1 changed after exposure to ethanol.

Alcohol Dehydrogenase↗
PubMed1994

Does Drosophila melanogaster use ethanol as an energy source during starvation?

The influence of starvation on activities of three enzymes (ADH, ODH and alpha GPDH) was studied in Drosophila melanogaster. The changes were compared in two inbred lines which had different allelic combinations at the Odh and Aldox loci. We also studied the effect of ethanol on media which contained no sucrose ("starvation conditions"). The results show that there are large differences in the larval and adult alcohol utilization. The alcohol content of the medium, in the absence of sugar, appeared to be toxic for the larvae, while the adults appeared to utilize it as an energy source. The two strains differed little in their responses to starvation or to the ethanol treatment applied under starvation conditions. We conclude that the degree of toxicity of ethanol is highly dependent on the presence of sucrose.

Alcohol Dehydrogenase↗
PubMed1991

Vector dissimilarity and clustering.

Based on the description of objects by m attributes, an m-element vector dissimilarity function is defined that, unlike scalar functions, retains the distinction among attributes. This function, which satisfies the conditions for a metric, allows the definition of betweenness, which can then be used for clustering. Applications to the subset-generation phase of conditional clustering and to nearest-neighbor-type algorithms are described.

Classification↗
PubMed1974

International reference study on the identification and scoring of human chromosome aberrations. Results of a WHO comparative study.

Chromosome aberrations in human peripheral blood are recognized parameters of cellular damage and are used as indicators of exposure to ionizing radiation and certain chemicals. However, significant interlaboratory variability exists in the results reported from different laboratories. The primary objective of the present study was to examine problems associated with the identification and analysis of chromosome aberrations. Significant interlaboratory variability was found to exist in the analysis of human chromosome spreads for induced interphase aberrations, apparently owing in part to differences in the selection and rejection of spreads for scoring and also in the recognition and classification of various types of aberrations. These differences are reflected in the dose-response relationships for aberrations as well as for damaged spreads. For damaged spreads the scoring variability appears to be relatively small. It is inferred that factors additional to differences in scoring may play a significant role in the large variation in the reported dose-response relationships both for ionizing radiation and for chemically induced aberrations.

Analysis of Variance↗
PubMedDate not supplied

The development of relational landmark use in six- to twelve-month-old infants in a spatial orientation task.

The ability to use the relations between visible landmarks to locate nonvisible goals (allocentric spatial coding) underlies success on a variety of everyday spatial orientation problems. Little is known about the development of true relational coding in infancy. Ninety-six 6-, 8.5- and 12-month-old infants were observed in a peekaboo paradigm in which they had to turn to a target location after displacement to a novel position and direction of facing. In a landmark condition, the target position was located between two landmarks, contrasted with a control condition in which no distinctive landmarks were provided. Six-month-old infants performed poorly in both conditions, 8.5-month-olds were significantly better with the landmarks, and 12-month-olds solved the task with or without landmarks. A follow-up study confirmed that the 8.5-month-olds used both landmarks to solve the task. This demonstration of allocentric spatial coding in 8.5-month-old infants shows earlier competence than that found in previous work in which only infants at the end of the first year were able to use landmarks relationally.

Age Factors↗