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Biomedical subjects

L Perdrix

Publications and source records attributed to L Perdrix.

5 recordsLinked to original sources
PubMed1991

Daflon as a cellular antioxidant and a membrane-stabilizing agent in human fibroblasts irradiated by ultraviolet A radiation.

Daflon is a strong inhibitor of Cu(2+)-induced arachidonic acid peroxidation, as revealed by the inhibition of thiobarbituric acid-reactive substance formation in mixed liposomes of phosphatidylcholine and arachidonic acid. Diosmin, the major Daflon constituent, is a good complexant of Cu2+ ions but not of Fe2+ ions. The Cu2+ complex formation may thus explain part of the antioxidant effect. However, Daflon is also a good quencher of the singlet oxygen-induced arachidonic acid peroxidation that does not involve metal ions. When cultured human fibroblasts are incubated with the diosmin-hesperidin mixture constituting Daflon, the latter is as efficient as vitamin E in inhibiting the ultraviolet A-induced lipid peroxidation and plasma membrane damage measured by lactate dehydrogenase release.

Antioxidants↗
PubMed1989

Protective effect of a purified flavonoid fraction against reactive oxygen radicals. In vivo and in vitro study.

S 5682 is a purified flavonoid fraction containing 90% diosmin (flavone derivative) and 10% hesperidin (flavanone derivative). In this study the scavenging properties of S 5682 on active oxygen radicals were demonstrated in vivo and in vitro. The activity of intravenous S 5682 was evaluated in the rat by measuring the degree of hyperglycemia provoked by an intravenous injection of alloxan, the metabolism of which produces active oxygen radicals which are toxic to B cells of the pancreas. S 5682 produced a decrease in the hyperglycemia in a dose-dependent manner (25 mg/kg and 50 mg/kg). The production in vitro of active oxygen radicals during phagocytosis by polymorphonuclear cells and macrophages is accompanied by emission of photons which can be amplified in the presence of luminol. Measurement of this chemiluminescence is a way of determining the quantity of active oxygen radicals generated in the medium, this may be reduced either by a decrease in production or by an increase in neutralisation through a reducing effect. Results demonstrated that S 5682 had a scavenging effect on active oxygen radicals for concentrations between 2 x 10(-8) mol/l and 2 x 10(-4) mol/l. The concentration of S 5682 reducing the chemiluminescence by 50% was 1.6 x 10(-5) mol/l. These scavenging properties imply that S 5682 has a pharmacological action on capillary hyperpermeability as well as an anti-inflammatory and anti-oedematous action.

Animals↗
PubMed1987

Effect of chronic treatment with a purified flavonoid fraction on inflammatory granuloma in the rat. Study of prostaglandin E2 and F2 alpha and thromboxane B2 release and histological changes.

S-5682 (Daflon-500 mg), a purified flavonoid fraction, consisting of 90% diosmin (a flavone derivative) and 10% hesperidin (a flavanone derivative), was administered to rats by intubation in the daily dose of 100 mg/d. 15 days after the start of treatment, polyurethane sponges were implanted in the subcutaneous connective tissue in the dorsolumbar region under rapid ether anaesthesia. Similar fragments of sponge were implanted in a group of control animals who received the vehicle (saccharose syrup) only, also by the oral route. The rats were sacrificed in fractions of 7 animals drawn from each of the two groups (control and treated) after 4, 8, 16 and 30 days (only 5 animals from each group on day 30) after implantation of the polyurethane sponges. The granulomas formed were removed, weighed and their prostaglandin (PG)E2, PGF2 alpha and thromboxane (Tx)B2 contents were determined. In addition a full cell count (polymorphs, lymphocytes, macrophages, plasmocytes and giant cells) was performed and the animals were histologically examined. The results show that treatment of the animals with S-5682 had the following effects: 1. A significant fall in the mean weight of the granulomas formed after 4 and 8 days was observed, reflecting inhibition of oedema formation during the early phase of the inflammatory reaction. 2. The synthesis of PGE2 (78.5% inhibition on day 4) and PGF2 alpha (45.2% on day 16) was inhibited. 3. There was very early inhibition of TxB2 synthesis (59.5% inhibition on day 4). 4. A later reduction in cell migration towards the inflammatory focus occurred which was statistically significant on day 16 (49.6% reduction in the total number of migrant cells). 5. Multiple histological aspects of the acute inflammatory reaction (diapedesis of polymorphs, lymphocytes, histiocytes and macrophages) and features of the chronic inflammatory reaction (newly formed microvascularisation of the granuloma tissue, perivascular oedema, presence of collagen fibres) were improved.

Animals↗
PubMedDate not supplied

Effects of Daflon 500 mg on bovine vessels contractility.

The effect of Daflon 500 mg, consisting of 90% diosmin and 10% hesperidin, on bovine veins and lymphatic vessels was shown. The recording of mechanical tension on metacarpal vein rings demonstrated a dose-effect relationship with an ED50 of 0.5 X 10(-6) M. This effect observed on isolated organs, is close to the tonic effect of Daflon 500 mg observed in human. An effect was also observed as demonstrated by the depolarisation curve obtained after administration of solutions containing increasing concentrations of potassium. The curve was shifted to the right which may indicate a decrease in potassium permeability in the presence of Daflon 500 mg. On lymphatic vessels isolated from mesenterium, the main effect was an increase in the frequency of spontaneous response which could explain the effect of Daflon 500 mg on lymph flow as shown previously. This dose-effect relationship could be due to an increase in the sensitivity of receptors to some agonists. However, this effect could be pleiotropic. This pleiotropic effect could involve the stimulation by Daflon 500 mg of other cellular mechanisms such as phosphatidylinositol metabolism, or more directly, the pacemaker activity of the cells which govern spontaneous activity, pacemaker activity being under the control of membrane potassium permeability. Some evidence about the action of Daflon 500 mg on potassium permeability could also be shown on other tissues.

Animals↗