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Biomedical subjects

L Perelmutter

Publications and source records attributed to L Perelmutter.

At least 37 records · Page 2Linked to original sources
PubMed1975

Production of rat homocytotropic antibodies using low dose, long term oral exposure to penicillin G.

Sera from 15 patients with immediate hypersensitivity reactions to penicillin G gave positive responses in the rat mast cell test (RMCT) indicating the presence of IgE-type antibodies in the sera. Five sera were from patients who had had reactions to penicillin 15 to 22 years previously without known re-exposure to this antigen. To explore the possibility that non-therapeutic exposure to penicillin may have produced continued sensitization in these patients, an animal model system was developed to explore the efficacy of low dose, long term oral exposure to penicillin G in rats for producing homocytotropic antibodies in these animals. It was found that when rats were given penicillin G in their drinking water at concentrations of 0.1 to 1 U/ml over a period of 1 to 3 months they produced serum IgE and IgGa antibodies. In addition, IgE antibodies were actively bound to the peritoneal mast cells of these animals. The presence of circulating or cell bound antibodies was detected using the rat mast cell test. It was also shown that rats given penicillin G orally for 1 month were more prone to antibody production after a single intramuscular injection of penicillin G compared to a control group receiving only the intramuscular injection of this antigen. The results of this study are discussed in terms of possible non-therapeutic sensitization towards penicillin G in the human population.

Administration, Oral↗
PubMed1975

Production of anaphylactic antibodies to drugs in experimental animals. I. Benzylpenicillin.

Studies were undertaken to determine if animals exposed to benzylpenicillin could produce anaphylactic antibodies to the drug moiety. For this purpose, Albino Wistar rats were divided into four groups. Two groups of animals were immunized with benzylpenicillin using either the intraperitoneal or the subcutaneous route. The remaining two groups were used for feeding experiments. The animals were bled upon termination of the experiments; their sera were collected and pooled per group. The pooled sera were tested for the presence of anaphylactic antibodies. Histamine release from rat mast cells under the appropriate conditions was used as an indicator of antibody production. Penicilloylpolylysine and benzylpenicillin were both employed to determine antibody specificity. It was found that sera from all groups of animals contained anaphylactic antibodies with specificity towards the penicilloyl group and "benzylpenicillin".

Administration, Oral↗
PubMed1975

Lymphocytotoxins and mixed leucocyte culture blocking factor activity in the plasma of uraemic patients undergoing haemodialysis.

Mixed leucocyte culture blocking factor activity (MLC-BFA) in the plasma of haemodialysis patients appears as a result of recent blood transfusions and is concentrated in IgG fractions of the blocking serum. Four patients neither developed lymphocytotoxins nor MLC-BFA in spite of having received multiple blood transfusions. Such 'unresponsive' recipients had an excellent clinical course upon receiving an allograft.

Blood Transfusion↗
PubMed1974

Mixed leucocyte culture blocking factor activity in the plasma of patients with cancer.

Plasma samples obtained from 74 patients with malignant disease were assayed for their ability to suppress or block the mixed lymphocyte reactivity of random normal donors. 24 of the 74 patients investigated had evidence of mixed lymphocyte culture blocking factoractivity (MLC-BFA). 19 of 33 patients with disseminated disease had evidence of MLD-BFA whereas in only 5 of 41 patients with localized disease was evidence of BFA found. There was a relationship between a history of previous blood transfusion inthe 6-month period prior to study and the presence of MLC-BFA in patients' plasma. Excluding this group of patients, where previous transfusion might have been responsible for MLC-BFA and excluding, too, that group of patients with a history of past pregnancy,there remained still 11 patients with evidence of BFA in their plasma. Nine of these11 had widely disseminated and progressiv disease. MLC-BFA was found in both IgM and IgG fractions of plasma. Plasma with MLC-BFA was found to suppress normalin vitro lymphocyte responses to mitogens PWM and PHA. The MLC-BFA assay utilized in this work may provide the means of monitoring cancer patients in order to detect 'blocking' antibody.

Antigen-Antibody Complex↗