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Biomedical subjects

L Pesti

Publications and source records attributed to L Pesti.

At least 19 recordsLinked to original sources
PubMed1984

Effects of tobacco smoke inhalation on the tracheo-bronchial microflora of rats.

The purpose of work was to study the effects of tobacco smoke on the airway flora in rats. A study on the regional distribution, total numbers and composition of the flora suggested the following: (a) Subacute smoke exposure intensified the penetration of the flora into deeper, normally sterile portions of the airways. (b) On smoking, the predominantly D. pneumoniae type flora of normal rats was found to shift to a form in which Hemophilus sp. was most frequent. (c) Exposure to low or high nicotine content cigarettes did not particularly modify these results.

Animals↗
PubMed1984

Local intestinal immune response to Escherichia coli heat-labile (LT) enterotoxin: LT antitoxin levels in healthy, diseased and antigen-fed pigs.

With purified LT toxin and IgA, specific anti-LT enterotoxin activity was demonstrated in small intestinal contents of 27 pigs. After 60 days of age, rise in intestinal LT antitoxin titer was observed. Feeding LT containing E. coli antigen increased LT antibody levels in the intestinal secretions, but decreased antibody titers in sera. In post-weaning E. coli diarrhea LT antibody levels in intestinal secretions and sera decreased significantly. This phenomenon can be related to the occurrence of the frequently observed post-weaning E. coli diarrhea.

Animals↗
PubMed1984

Immunological properties of porcine enterotoxigenic Escherichia coli heat-stable (ST) enterotoxins.

Cross-linking semipurified and purified heat-stable enterotoxin (2023 ST) to carrier proteins with glutaraldehyde pigs and rabbits were immunized. With ST antisera thus raised, a relatively simple, two-step method for isolation of ST is described. The method involved immunoadsorbent column chromatography procedure, and elution of the retained material with 6 M urea at pH 3.0 yielded purified ST with enterotoxin activity, controlled in suckling mouse test. ST purity was checked with a special staining technique in PAGE, anionic-exchange chromatography and immunodiffusion.

Animals↗
PubMed1981

Staining technique for peptides of Escherichia coli heat-stable enterotoxin.

Crude and purified Escherichia coli heat-stable enterotoxin was stained directly in polyacrylamide gel rods after electrophoresis with Coomassie brilliant blue G-250 dissolved in 1 N H2SO4--one-month volume of 10 N KOH-12% trichloroacetic acid. The stained heat-stable toxin bands were partially characterized.

Enterotoxins↗