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Biomedical subjects

L Pfister

Publications and source records attributed to L Pfister.

13 recordsLinked to original sources
PubMed2005

Spatial variability of trends in hydrological extremes induced by orographically enhanced rainfall events due to westerly atmospheric circulations.

Since the mid 1970s, the number of days with westerly atmospheric circulations has strongly increased during winter months. As a consequence, rainfall totals, rainfall event duration and intensity have been subject to significant positive trends throughout the Mosel river basin. However, the trends identified through the non-parametrical test named Kendall's tau have shown to be spatially varying. The intensity of the trends appears to be directly linked to orographic obstacles that are well known to have a strong influence on average rainfall totals. A direct consequence of the changes having affected winter rainfall under westerly atmospheric circulations on the one hand and the spatial variability of these changes on the other hand, is a spatially varying positive trend in maximum winter streamflow. Thus, even though a clear large-scale change has affected winter rainfall over the past decades, its intensity is either strongly moderated or enhanced by orographic obstacles. The related changes in streamflow are directly dependent on the spatial variability of the changed rainfall characteristics.

Air Movements↗
PubMed2005

Climatic change and the effect on hydrology and water management in the Rhine basin.

There has been a marked increase in recent years in the resources dedicated to investigating the problems arising from climate change with respect to hydrology and to some extent to water resource management. Many of these studies are concentrated on the river Rhine basin. In order to review the actual state of scientific findings a workshop, organised by the International Commission for the Hydrology of the Rhine (CHR), was held on 24 and 25 June 2003. The invited experts discussed the following topics: observed variability in climatic and hydrological data, the development and interpretation of climate scenarios, and assessment of changes in the discharge regimes and the occurrence of hydrological extremes. Based on the workshop the question of whether or not current knowledge and procedural strategies can be used as a basis for future water management was evaluated by an expert group of the CHR. In this paper the process of decision-making with regard to flood management is analysed. Hints are given to convince the decision-makers to take into account the impacts of climate change in water resources management. The special challenges arising in international river basins are discussed.

Climate↗
PubMed2001

Community-acquired pneumonia. A prospective outpatient study.

We initiated a prospective study with a group of practitioners to assess the etiology, clinical presentation, and outcome of community-acquired pneumonia in patients diagnosed in the outpatient setting. All patients with signs and symptoms suggestive of pneumonia and an infiltrate on chest X-ray underwent an extensive standard workup and were followed over 4 weeks. Over a 4-year period, 184 patients were eligible, of whom 170 (age range, 15-96 yr; median, 43 yr) were included and analyzed. In 78 (46%), no etiologic agent could be demonstrated. In the remaining 92 patients, 107 etiologic agents were implicated: 43 were due to "pyogenic" bacteria (39 Streptococcus pneumoniae, 3 Haemophilus spp., 1 Streptococcus spp.), 39 were due to "atypical" bacteria (24 Mycoplasma pneumoniae, 9 Chlamydia pneumoniae, 4 Coxiella burnetii, 2 Legionella spp.), and 25 were due to viruses (20 influenza viruses and 5 other respiratory viruses). There were only a few statistically significant clinical differences between the different etiologic categories (higher age and comorbidities in viral or in episodes of undetermined etiology, higher neutrophil counts in "pyogenic" episodes, more frequent bilateral and interstitial infiltrates in viral episodes). There were 2 deaths, both in patients with advanced age (83 and 86 years old), and several comorbidities. Only 14 patients (8.2%) required hospitalization. In 6 patients (3.4%), the pneumonia episode uncovered a local neoplasia. This study shows that most cases of community-acquired pneumonia have a favorable outcome and can be successfully managed in an outpatient setting. Moreover, in the absence of rapid and reliable clinical or laboratory tests to establish a definite etiologic diagnosis at presentation, the spectrum of the etiologic agents suggest that initial antibiotic therapy should cover both S. pneumoniae and atypical bacteria, as well as possible influenza viruses during the epidemic season.

Adolescent↗
PubMed1996

Immunoglobulin heavy chain variable region gene usage in bone marrow transplant recipients: lack of somatic mutation indicates a maturational arrest.

Many recipients of bone marrow transplant (BMT) make normal amounts of serum immunoglobulin but are deficient in generating specific antibody responses to exogenous stimuli. To determine if abnormal usage of VH genes contributes to this immunodeficiency, the usage of VH genes was determined in peripheral blood B cells of four BMT recipients, two of whom had developed chronic graft versus host disease. The pattern of usage of VH3 or VH4 genes assessed at either 90 days or approximately 1 year after transplant was similar to that observed in healthy subjects and was marked by the over utilization of two elements, one VH3 and one VH4. However, the repertoires of each of the four BMT recipients appeared to be less complex than the repertoires of healthy subjects. The differences were a consequence of the accumulation of somatic mutations among rearrangements in the controls but not in the BMT recipients. The failure to accumulate somatic mutations in rearranged VH genes is consistent with a defect in antigen driven B-cell responses. These results indicate the although the VH gene content of the repertoire has normalized by 90 days posttransplant, a maturational arrest in B-cell differentiation associated with antigen activation persists for at least 1 year after BMT.

Antibody Formation↗
PubMed1995

Representation of rearranged VH gene segments in the human adult antibody repertoire.

The heavy chain variable region composition of the human adult Ab repertoire is poorly defined, but recent evidence suggests that peripheral blood B cells may express a nonstochastic assortment of VH genes. In this study, the contribution of individual VH gene segments to the human Ab repertoire has been assessed. As a measure of VH gene utilization, the frequency of occurrence of eight individual VH3 gene segments contained in rearrangements was assessed in the peripheral blood B cells of two adult subjects. In addition, the frequency of occurrence of rearrangements containing nine individual VH4 gene segments was analyzed in one of the subjects. More than 2500 independent rearrangements were analyzed. For controls, amplifications and subsequent identification of nonrearranged VH3 and VH4 genes from the same individuals were also performed. The results of this germ-line analysis indicated that approximately 25 VH3 gene segments and nine VH4 gene segments could be amplified quantitatively. However, usage of elements was not uniform; one VH3 element, V3-23, and one VH4 element, V4-34, were represented among rearrangements more frequently than were other members of their respective families. This pattern of VH utilization was apparent in B cells isolated from the same subject after an 8-mo interval, indicating the relative stability of the repertoire over time. These results indicate that the adult human Ab repertoire is dominated by a few VH genes demonstrating a pattern of nonrandom utilization that could involve preferential rearrangement and/or receptor-dependent selection.

Adult↗
PubMed1983

[Ultrasonic diagnosis in hydrocephalus].

Sonographic evaluation of the brain offers a noninvasive, safe and inexpensive method in diagnosis and follow-up of hydrocephalus associated with meningomyelocele. Cranial computed tomography should be reserved for special questions such as assessment of bony structures, subdural effusions or if fontanelles and sutures are already closed. Ultrasound of the neonatal head is completely harmless, repeated examinations can easily be performed and sedation of the infant is usually not necessary. The method of ultrasound sections in different directions is demonstrated and compared to axial CT sections. Value, effectiveness and diagnostic interpretation of the method are demonstrated in four cases: 1. Small lumbar meningomyelocele with mild hydrocephalus without enlargement on follow-up. 2. Giant spinal defect with kyphoskoliosis, marked hydrocephalus and associated ventricle abnormalities. 3. Sonographic follow-up after ventriculo-atrial shunting in a patient with congenital hydrocephalus. 4. Cerebrospinal fluid abdominal pseudocyst after ventriculo-peritoneal shunting. Thus ultrasound is an excellent method for following ventricular size and shunt-function in meningomyelocele-patients.

Abdomen↗
PubMed1982

[Evaluation of aortic dissection by transverse computerized tomography (CT scan)].

Twenty patients with dissection of the thoracic aorta were studied by CT-scanning. In 9 patients the diagnosis was previously documented by aortography, while in the other 11 patients the diagnosis of aortic dissection was first established by CT-scanning. The latter demonstrated the characteristic signs of aortic dissection and particularly the following features: a) the false channel (95%) and the intimal flap (90%), b) the displacement of aortic wall calcifications (60%), especially when the false channel is partially thrombosed (80%). In 19 patients, a bolus injection of contrast medium was performed. In the majority of cases opacification of the false channel occurred only partially, with some delay and with a non-homogeneous pattern. Six out of the 20 patients underwent one or more follow-up examinations performed between 15 days and 2 years later. CT-scanning clearly demonstrated the late persistence of a patent false channel and particularly the delayed thrombosis, even after surgical treatment of aortic dissection. CT-scanning appears to be a reliable non-invasive method for - establishing the diagnosis of aortic dissection, - delineating the extent of dissection, - follow-up of evolution after surgical or medical therapy.

Aged↗
PubMed1979

Preclinical radiobiology with pions: RBE--values for mouse skin damage as a function of single doses of pions.

The macroscopic reaction of the mouse skin was used to derive RBE values for negative pi-Mesons. Hind limbs of mice were irradiated with pions or X-rays. The pions were produced by the 590 MeV accelerator of the Schweizerisches Institut für Nuklearforschung (SIN). Early reaction was assessed over a period of 6--30 days after irradiation with single doses (20--45 Gy). The radiation damage was scored using an arbitrary scale of effect. The time pattern of development of the skin reaction and the subsequent healing after exposure both to pions and X-rays were similar, indicating that depletion and repopulation of the basal cells of the skin were comparable, both after pions and X-rays. RBE values as a function of pion doses at the peak (dose maximum), plateau and at the postpeak (12 mm downstream of the dose maximum) were computed with nonparametric statistical methods. The RBE at the peak and at the plateau relative to X-rays of the same dose rate was 1.15--1.25 and 0.85, respectively. The RBE of peak pions manifested a marked dependence on dose, when plateau pions were chosen as reference radiation. In this experiment there was no significant difference in RBE between peak and postpeak. The importance of some experimental condition (dose rate, irradiation volume) is discussed.

Animals↗