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Biomedical subjects

L Pinkus

Publications and source records attributed to L Pinkus.

5 recordsLinked to original sources

The mechanisms of repression and isolation in multiple sclerosis as regulators of personality system: a clinical study.

One of the fundamental dynamics of psychic adaptation to both intra-psychic and relational stimuli is the defense mechanism of repression in correlation with that of isolation. Our work is concerned with testing whether the use of these mechanisms can constitute, in the case of MS patients, one of the factors which would explain various psychological reactions which are inadequate or pathological. It is to be expected that the trend of these defense mechanisms will be significantly altered in the case of MS patients. In our research we evaluated the Rorschach's Index of Repressive Style (Levine and Spivack, 1964) and the Index of Isolation (Crisi and Caviglia, 1987). The experimental group consisted of 40 MS subjects (20 males and 20 females, age range 20-47) not exceeding level 5 on Kurtzke's Disability Scale. 40 healthy subjects (20 males and 20 females, age range 22-50) were used as a control group. The MS group showed more severe signs of repression than the control group; the MS group showed also more severe signs of activation of the defense mechanism of isolation.

Adaptation, Psychological↗

Purification and properties of estrogen-responsive cytoplasmic thymidine kinase from human breast cancer.

The effect of 17 beta-estradiol on cytoplasmic thymidine kinase activity was studied in MCF-7, a human breast cancer cell line in culture which responds to estrogens with an increase in the rate of growth. Levels of 17 beta-estradiol which maximally stimulate [3H]thymidine incorporation into DNA also maximally stimulate thymidine kinase activity. The Vmax for thymidine increased while the Km was not affected by estrogen stimulation when performed on nonpurified enzyme. Tamoxifen, an antiestrogen, decreased the specific activity of the enzyme. To further study its hormonal regulation, cytoplasmic thymidine kinase was purified greater than 2000-fold by affinity column chromatography. The purified preparation migrated in one band to a pI of 8.5 on an isoelectric focusing gel. The purified thymidine kinase was further characterized by examining its molecular weight, pH optimum, heat stability, utilization of phosphate donors, inhibition by nucleotides, and the effect of pyrimidine nucleoside analogs.

Breast Neoplasms↗