[Characterization of microbial species of the Micrococcaceae family by polyacrylamide gel electrophoresis].
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Bacterial Proteins↗
Biomedical subjects
L Pitzurra
Publications and source records attributed to L Pitzurra.
Candida albicans stress mannoprotein, SMP200, enhances tumour necrosis factor secretion in the murine macrophage cell line ANA-1.
Tumour necrosis factor (TNF) secretory activity has been studied in the murine macrophage cell line ANA-1 following in vitro exposure to Candida albicans 200 kDa stress mannoprotein (SMP200). Treatment of ANA-1 murine macrophages with 200 kDa stress mannoprotein results in increased TNF secretion. The phenomenon is (i) dose- and time-dependent, (ii) abrogated by 200 kDa stress mannoprotein preincubation with a specific monoclonal antibody, and (iii) dependent on intact murine macrophage Ca2+/calmodulin-dependent protein kinase function.
Animals↗
Microbial growth and air pollution in carbonate rock weathering. Preliminary results of a in situ experimental study.
Preliminary results on limestone weathering caused by air pollution and microbial colonization are presented in this study. Outdoor exposure experimental assays were performed on Scaglia limestone samples. Samples were exposed in two areas in Perugia (Italy) that differ for degree of urban air pollution. At different times of exposure, ranging from 1 to 12 months, microbial contamination and textural modifications of sampled surfaces were evaluated by microbiological procedures, X-ray diffraction, and scanning electron microscopy. After one year of exposure a significant fungal colonization and the presence of weathering products (i.e. gypsum) were detected on sampled surfaces.
Air Pollutants↗
[Filaments of the "Candida albicans" in human serum (author's transl)].
A study on human serum activity on Candida albicans cells has been carried out. In smear microcultures the microcolonies morphogenesis and the thread formation in the presence of serum, as well as the reappearance of normal yeast cells after re-growth in normal nutrient agar has been assessed. The serum globulin-fraction appeared to have the greatest germ-tube inducing activity: an heat-sensitive quality that went lost after 30 minutes at 100 degrees C. The multiplying activity of the Candida cells was almost completely abolished in the presence of serum. Realying on these experimental findings the AA. suggest that a demaging factor may be present in sera, linked to the globulin protein fraction, that hinders the Candida cell-wall normal synthesis: hence follows the appearance of threads, mostly deprived of multiplying activity. A condition in some way akin to the well known filament inducing activity of penicillin on Gram-negative bacteria.
Blood↗
[Evaluation of the humoral immunity in patients with cancer of the larynx].
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Adult↗
Resistance induced by concanavalin A and phytohaemagglutinin P against tetanus toxin in mice.
The effect of concanavalin A (ConA), phytohaemagglutinin P (PHA) and Limulus polyphemus haemocyanin (LPH) on the lethal activity of tetanus toxin (TT) is reported. C3H mice treated s.c. with ConA or PHA but not with LPH from 48 h before to 12 h after s.c. TT challenge showed a significant increase in median survival time compared to control mice inoculated with toxin alone. This protective effect was also obtained when PHA or ConA was administered by the i.p. route, TT being injected s.c. In further studies, mice treated with ConA or PHA by different routes (s.c., i.p. or i.v.) were challenged s.c. with graded minimal lethal doses of TT, with or without i.p. administration of horse antitetanus serum (HATS) 24 h after toxin inoculation. The mice treated with ConA or PHA + HATS showed a significantly increased survival rate and a higher percentage of cured mice with respect to control animals treated with lectins alone. In contrast, the mice challenged with TT and treated with HATS alone did not show any increased survival with respect to untreated controls. Sera from ConA- or PHA-treated mice were unable to neutralize the TT. Immune depression in mice by total-body irradiation (400 R) did not abolish the protective activity of the lectins. These results show that in vivo treatment of mice with ConA or PHA but not with LPH can protect against the lethal effects of TT.
Animals↗
Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response.
In the present study, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques, the patterns of proteins extracted from C. neoformans yeast cells were analysed. A major 105 kilodalton (kDa) antigen that binds to Concanavalin A and cross-reacts with anti-mannan antibodies was identified. The 105 kDa mannoprotein, highly expressed in the acapsular mutant of C. neoformans with respect to the encapsulated strain, is involved in the lymphoproliferative response of T lymphocytes to Cryptococcus-sensitized monocytes.
Antibodies↗