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L Putowski

Publications and source records attributed to L Putowski.

8 recordsLinked to original sources
PubMed2001

[Differences in human and rat FSH receptors promote activity as a result of the transcriptional factors: E2F1, E2F4 and E2F5 overexpression].

UNLABELLED: FSH-R expression in granulosa cells varies during the course of ovarian ontogenesis, as well as, during each menstrual cycle. Expression of follicle-stimulating hormone receptors (FSH-R) on Sertoli cells of the testis and ovarian granulosa cells depend on many paracrine and autocrine factors. The modulation of FSH-R synthesis is accomplished via a number of mechanisms including regulation of the promoter activity. Little is known about factors involved in control of FSH-R transcription in different species. The aim of the present study was to investigate differences in the regulation of human and rat FSH-R promoter activity by E2F transcriptional factors. MATERIAL AND METHODS: The 5'-flanking regions of human and rat FSH receptor gene subcloned in the pGL3 plasmid were transiently transfected into cultured CHO cells and rat granulosa cells. Rat granulosa cells were obtained by puncturing ovaries from DES primed immature Sprague-Dawley rats. Promoter activity was determined by measuring firefly luciferase luminescence of the cell lysate. Transfection efficiency was normalized by the renilla luciferase activity generated by co-transfected pRL-CMV vector. In order to determine the influence of E2F1, E2F4 and E2F5, on FSH-R promoter activity, cells were transfected either with promoter construct alone or with its mixture with selected expression vector. RESULTS: Rat FSH-R promoter construct (-1033/+6 bp) and human FSH-R promoter construct (-1485/-1 bp) were both active in transfected cells. Overexpression of E2F1 protein decreased both, human and rat wild type FSH-R promoter activity. Overexpression of E2F4 did not affect neither rat nor human FSH-R gene transcription. Expression vector for E2F5 increased both, human and rat, FSH-R promoter activity. Folds of increase were markedly higher in case of rat FSH-R construct transfection, comparing to human FSH-R promoter. CONCLUSIONS: Results suggest, that the E2F1 and E2F5 factors might play an opposite role in the regulation of FSH-R promoter activity. More pronounced stimulatory effect of E2F5 on the rat FSH-R can be explained by the presence of E2F site in the promoter. Since there is no E2F sensitive element in the human FSH-R promoter sequence, E2F1 and E2F5 can also indirectly influence FSH-R promoter activity.

Animals↗
PubMed1995

[The effect of TNF-alpha (tumor necrosis factor-alpha) on progesterone synthesis in vitro by cells from ovarian follicular fluid in women].

Follicular fluid was obtained through the ovarian follicle puncture during laparotomy in seven women. Follicular cells were cultured for 66 hours in the presence of TNF-alpha or lipopolysaccharides. Concentration of FSH, LH and prolactin in follicular fluid and progesterone levels in culture medium were estimated. TNF-alpha (100 ng/ml medium) diminished progesterone production in 6 hours tissue culture but reduced its concentration in 18 and 42 hours culture in comparison to the controls. Correlation was found between FSH level in follicular fluid and percentage of progesterone concentration change in experiments with TNF-alpha 100 mg/ml up to 42 hours and with TNF-alpha 500 ng/ml up to 18 hours. Lipopolysaccharides (10 micrograms/ml) did not influence progesterone production in the tissue culture.

Adolescent↗
PubMed1993

[Fertility after adnexal surgery].

Among 64 women undergoing tubal-ovarian surgery 40.6% became pregnant spontaneously. Because of 47% of the remainder patients demonstrated anovulation the induction of ovulation was performed except the cases with hypergonadotrophic hypogonadism (9.4%). After treatment the total population with ovulatory cycles increased to 73.4% and pregnancy ratio upwards 48%. The pregnancy was noted in the half of the patients with endometriosis and all multiparas and after ectopic pregnancy.

Adult↗
PubMed1993

[Analysis of cause and test treatment of amenorrhea in teenagers].

The population of 49 young, secondary amenorrheic women aged 17 to 20 was divided according to reaction on i.m. injection of progesterone in oil. The positive category was subdivided into I--polycystic ovarian disease with elevated serum LH and II--hypothalamic-pituitary dysfunction (HPD) with normal (or decreased) level of LH. The negative category consists of subgroup, III--hypothalamic-pituitary failure (HPF) with normal or low serum FSH and IV--ovarian failure with elevated level of FSH (two patients were noted). The bleeding response was obtain in two third of the patients, and also two third of them ovulated after clomiphene citrate treatment, including all six women with POC. The ovulatory response in patients with hypothalamic pituitary failure was noted in more than 40%. The low concentration of serum FSH (below 5 mU/ml) was more frequent in HPF (57%) in comparison with HPD (48%). The resistance to clomiphene citrate treatment was correspondingly 62.5% and 70%. When the ovulation occurred no difference in the regular function of corpus luteum in POC, HPD and HPF patients was noted.

Adolescent↗
PubMed1992

[Production of progesterone in short term tissue culture by follicular cells of women].

After volume and content of the follicular cells were determined the concentration of LH, FSH and prolactin was estimated in the ovarian follicular fluid taken during laparotomy of 17 women (ages 14-43). The sixty six hour follicular fluid cells cultures were established and the concentration of the medium progesterone were assessed by means of radioimmunological methods. The ability of cells to produce progesterone was demonstrated in 3/4 of the cases. It has been found that the progesterone producing cells come from the follicles with significantly higher LH and prolactin concentration. The positive correlation was observed between FSH concentration in follicular fluid and culture progesterone production by the cells in 42 and 66 hour of the experiments.

Adolescent↗
PubMedDate not supplied

Rat ovarian insulin-like growth factor binding protein-4: a hormone-dependent granulosa cell-derived antigonadotropin.

OBJECTIVE: To assess the in vivo regulation of ovarian insulin-like growth factor binding protein-4 (IGFBP-4) mRNA expression by gonadotropins and estrogen. METHODS: Whole ovarian RNA, obtained from two models of follicular development, was extracted and analyzed by Northern blotting. Immature rats were treated with pregnant more serum gonadotropin (PMSG) followed 48 hours later with hCG, or alternatively were hypophysectomized and treated with FSH and/or diethylstilbestrol (DES). Localization of IGFBP-4 expression was assessed in the former study by in situ hybridization. Finally, the ability of human IGFBP-4 to antagonize FSH-stimulated progesterone accumulation was assessed in vitro. RESULTS: The ovarian content of IGFBP-4 transcripts increased threefold (P < .05) at 12 hours after PMSG but was near baseline at 24 and 48 hours. The abundance of IGFBP-4 mRNA increased (P < .05) again at 6 and 24 hours after hCG. The expression of IGFBP-4 was localized to granulosa cells of preantral (untreated) and small antral (12 hours after PMSG) follicles. No IGFBP-4 expression was noted in large (gonadotropin-primed) antral follicles. Hypophysectomy increased (P < .05) the ovarian content of IGFBP-4 mRNA by 1.5-fold, an effect further enhanced (1.8-fold; P < .05) by the provision of FSH and DES. In vitro studies revealed the ability of increasing concentrations (0.01-1 microgram/mL) of recombinant human IGFBP-4 to inhibit the FSH-supported accumulation of progesterone. CONCLUSION: Increased expression after administration of PMSG, hCG, and FSH/DES suggests that IGFBP-4 is a dynamic and hormonally responsive component of the ovarian cycle. The lack of expression in preovulatory follicles and its antigonadotropic actions in vitro imply that the attenuated expression of IGFBP-4 may constitute a requirement for successful follicular maturation.

Animals↗