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L R Berger

Publications and source records attributed to L R Berger.

61 records · Page 4Linked to original sources

Complex lipids of Rhodomicrobium vannielii.

Eight components, seven of which contained phosphorus, were found in the phospholipid fraction of Rhodomicrobium vannielii. The major components were lipoamino acid (o-ornithine ester of phosphatidyl glycerol, 46.5%) and phosphatidyl choline (26.5%). The other six components were phosphatidyl glycerol (9.7%), bisphosphatidic acid (6.7%), phosphatidyl ethanolamine (4.5%), phosphatidic acid (1.8%), lysophosphatidyl glycerol-o-ornithine ester (3.2%), and N,N-ornithine amide of unidentified fatty acid (0.95%). Total phospholipid accounted for 4.2% of cell dry weight. The major fatty acid was vaccenic acid, C(18:1), which accounted for approximately 90% of the total fatty acids of the complex lipid fraction. The other four fatty acids were C(16:0) (6.25%), C(18:0) (3.8%), C(14:0) (0.7%), and C(16:1) (0.35%). The sulfolipid content was 0.01% of the cell dry weight or 0.14 mumoles per g of dried cells, assuming that its fatty acid component is vaccenic acid. No steroids were detected.

Bacteria↗

Fatty acids of extractable and bound lipids of Rhodomicrobium vannielii.

Cells of Rhodomicrobium vannielii grown at 29 C in a lactate-containing medium were extracted at room temperature with organic solvents. The extractable fraction contained the bulk of the simple lipid (1.87% of cell dry weight) and complex lipids (phospholipids, 4.2%; sulfolipid, 0.01%), coenzyme Q (0.09%), and pigments (carotenoids 1.2%; bacteriochlorophyll, 1.9%). The cell residue contained the bound lipids (nonpolar fatty acid fraction, 1.86%; polar hydroxy fatty acids, 0.49%). The residue also contained poly-beta-hydroxybutyric acid (0.2%), which was extracted in boiling chloroform. In both the simple and complex lipids, vaccenic acid (11-octadecenoic acid) was the largest single component (approximately 90% in each fraction). The fatty acids of the bound lipid contained 35% vaccenic acid, even- and odd-numbered saturated and unsaturated straight-chain fatty acids, cyclopropane-, branched-, and alpha- and beta-hydroxy fatty acids. The extractable lipids contained only straight-chain saturated and unsaturated even-numbered fatty acids. Nearly 60% of hydroxy fatty acid fraction was alpha-hydroxydodecanoic acid (24%) and beta-hydroxydodecanoic acid (34.5%). Coenzyme Q was crystallized and identified as Q(9) on the basis of melting point and chromatographic properties. Q(10) had been previously reported.

Bacteria↗

Training injury control practitioners: the Indian Health Service model.

Many individuals practicing injury control have not received specific training for their work, in large part because of a scarcity of training opportunities. Consistent with its mission of "raising the health status of American Indian and Alaska Native people to the highest possible level," the Indian Health Service (IHS) created an innovative training program for federal and tribal employees. The model emphasizes training that is practical and can be applied immediately to community interventions. Many features of the IHS training model have broad applicability to other settings. These features include the use of experiential instruction, preceptors, and community case studies to train individuals from diverse cultural and educational backgrounds; educational strategies for employed adults; and courses that promote community empowerment. The development of IHS training courses are guided by community input, epidemiological data, advances in knowledge, and program evaluations. Courses range from a half-day "minicourse" to a full-year fellowship program. The success of the training model is evident in programs instituted by IHS Injury Prevention Specialist Fellowship graduates, whose projects have ranged from drowning prevention in Alaska to fire safety in North Dakota. The IHS training model could be applied in a variety of other community-based settings, but it is most relevant to programs that train individuals from diverse backgrounds who are not full-time students and programs that make community needs an organizational priority.

Accident Prevention↗

Carbon monoxide hazards in rural Alaskan homes.

Alaska has the highest age-adjusted death rate from unintentional carbon monoxide poisoning in the nation. We conducted a study in five villages to determine carbon monoxide levels and sources. Nearly 10% (10/105) of the homes had elevated levels. Improperly vented propane Paloma water heaters were most commonly responsible and produced the highest levels of carbon monoxide. Other sources were leaking pipes from wood stoves and stoves that had been left on for several hours. We recommend that Paloma water heaters not be used where freezing temperatures create a risk for carbon monoxide poisoning, fires, and explosion. Safety education campaigns should note the risks of Paloma water heaters and defective stove pipes, and the need for increased kitchen ventilation during prolonged cooking. Medical workers need to be informed of the prevalence of carbon monoxide exposures. Homeowners can install carbon monoxide detectors, although expense and false alarms remain barriers to their use.

Air Pollution, Indoor↗