Screening for mutations in the gene encoding factor IX.
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Biomedical subjects
L R Nielsen
Publications and source records attributed to L R Nielsen.
Analysis of the decreased NK (natural killer) activity in lung cancer patients, using whole blood versus separated mononuclear cells.
The aim of this study was to analyze whether a whole blood assay would give a more correct measure of NK activity than assays using separated mononuclear cells (SMNC). We found that the NK activity of whole blood was higher than the NK activity of SMNC in the 28 lung cancer patients investigated (p = 0.01), whereas this difference between the assays could not be demonstrated in the 29 healthy controls. Since no differences were found between the NK activity of washed blood, SMNC, and monocyte-depleted lymphoid cells, there was no indication that the lower NK activity of SMNC in comparison with whole blood was due to cell loss or to a systematic disturbing effect due to monocytes. The possible effect of plasma factors on the whole blood NK activity was analyzed by comparing whole blood and washed blood. The NK activity of whole blood was increased in comparison with washed blood in the lung cancer patients (p less than 0.0001) indicating a stimulatory effect of plasma. Further, the finding that the reactive capability of lymphocytes from cancer patients was higher than in controls could indicate preactivation of the lymphocytes from the cancer patients due to the presence of stimulatory plasma factors. The NK activity of lung cancer patients was lower than the NK activity of healthy controls. The difference was found to be smaller with whole blood than with SMNC as effector cells, although both differences were significant. The decreased NK activity of cancer patients could be due to blocking immune complexes (IC), but we found no evidence for circulating or cell-bound IC in the lung cancer patients.
Adult↗
A polyclonal IgM-RF enzyme-linked immunosorbent assay for the detection of circulating immune complexes.
A microplate-adapted polyclonal IgM-rheumatoid factor enzyme-linked immunosorbent assay (pIgM-RF ELISA) for the detection of circulating immune complexes (cIC) is presented. The assay involves the competitive binding of cIC and horseradish peroxidase conjugated aggregated human IgG (HRP-AHG) to solid-phase bound polyclonal IgM-RF (pIgM-RF). Aggregated human IgG (AHG) inhibited the binding of HRP-AHG to pIgM-RF in a dose-dependent way. The detection limit of the assay was about 125 ng AHG/ml diluted serum. The coefficients of variation for the assay varied from 5.0 to 14.7% for intra-assay runs and from 4.5 to 13.8% for inter-assay runs. The levels of cIC in sera from 29 patients with systemic lupus erythematosus (SLE), 85 untreated patients with breast cancer and 105 blood bank donors were studied by the pIgM-RF ELISA. Increased levels of cIC were demonstrated in 41.4% of the SLE group, in 8.2% of the breast cancer group, and in 1.9% of the normal control group. The difference in cIC activity between the SLE group and the normal control group was statistically significant.
Adult↗
Lymphocyte glutathione peroxidase activity during exacerbations in multiple sclerosis.
Glutathione peroxidase, one of the major antioxidants in the human brain, has been found to have decreased activity in patients suffering from multiple sclerosis (MS). This study compares the activity of lymphocyte glutathione peroxidase (L-GSH-px) in MS patients suffering from acute relapses with clinically stable MS patients and with control patients referred with nondemyelinating neurological diseases. All three groups showed an increase of mean enzymatic activity (MEA) during the observation period. The highest MEA in this study was observed in the MS groups. However, there were no significant differences in the L-GSH-px activity in the three groups. These results are not in accordance with previous investigations, and the need for further research in this field is emphasized.
Adult↗