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L R Trabulsi

Publications and source records attributed to L R Trabulsi.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Lomefloxacin activity against 2,813 clinical isolates: a collaborative study at three medical centers in Brazil.

The in vitro activity of lomefloxacin was compared to norfloxacin and two parenteral drugs against 2,813 clinical isolates at three medical centers in Brazil. Considering MICs less than or equal to 4 micrograms/ml as the criterion, lomefloxacin inhibited 97.9% of all enterobacteriaceae, 100% of staphylococci, 100% of Neisseria gonorrhoeae, 100% of Campylobacter pylori, 81% of Pseudomonas aeruginosa, and 79% of Streptococcus spp. Norfloxacin was generally comparable in potency and spectrum, but cefotaxime and gentamicin were less effective because of high-resistance prevalence in some species. The highest rates of fluoro-quinolone resistance among the Enterobacteriaceae were in the Proteus-Providencia group of species.

Anti-Infective Agents↗
PubMedDate not supplied

Identification of a new enteroinvasive Escherichia coli strain.

We isolated an enteroinvasive Escherichia coli strain which did not react with antisera raised against all known E. coli serogroups, Shigella groups A, B, C and D or provisional Shigella serotypes. We propose the provisional designation of Escherichia coli MG for this strain, until the identification of its O antigen is established.

Adult↗
PubMedDate not supplied

Inhibition of enteropathogenic Escherichia coli (EPEC) adherence to HEp-2 cells by bovine colostrum and milk.

BACKGROUND: enteropathogenic Escherichia coli (EPEC) is the main etiological agent of infantile diarrhea in Brazil and other developing countries. Human milk IgA protects newborn intestinal mucosa by inhibiting bacterial adhesion to epithelial cells and this effect is shown by in vitro assays of EPEC adhesion to HEp-2 cultured cells. Bovine milk, if effective in promoting this protection, could be an useful tool in the absence of the natural breastfeeding, in high-risk nurseries or in hospital infections. METHODS: the effect of colostrum, milk, and serum from dairy cows on the adherence to EPEC to HEp-2 cells was investigated. Colostrum from immunized and control animals and industrialized milk formulas were fractionated through a membrane device with a molecular weight cut off 10 kDa. The high molecular weight fraction (HMWF) of bovine colostrum was depleted of IgG through an affinity column and absorbed with an EPEC adherent strain. Antibodies were searched by ELISA and immunoblotting (IB). RESULTS: colostrum and milk from EPEC-immunized animals showed and inhibitory activity on adherence similar to that of control non-immunized animals. The inhibitory effect on adhesion was related to the HMWF. IgG-depleted colostrum partially retained the inhibitory effect, whereas IgG-rich eluate lost this property. The EPEC-absorbed fraction retained the inhibitory property. Industrialized milk formulas and respective HMWF also inhibited bacterial adherence. In IB assays, colostrum and milk samples from immunized animals recognized proteins of 30-40 kDa and 94 kDa, a molecular weight consistent with the adhesin intimin, in EPEC extracts. CONCLUSIONS: the inhibitory effect of EPEC adherence may be mediated by HMWF components, and IgG was not the only component responsible for this phenomenon.

Adhesins, Bacterial↗
PubMedDate not supplied

[The effect of the supernatants of enteropathogenic Escherichia coli strains on the intestinal transport of sodium in rats "in vivo"].

We studied the effect of the supernatants of 2 to 5 samples of one of the following serotypes of EPC: 026:H, 086:H34, 0111:H-, 0119:H6, 0126:H21, 0128ab:H35 and 0142:H-, isolated from the stools of infants with gastroenteritis. We also studied 3 samples of the serotype 0157:HNT, that produces the Shiga-like toxin. The following control solutions were selected 1-SC1 (glucose-saline solution); 2-SC2 (TSB broth culture solution); 3-SC3 (E.coli K12 711 culture supernatant, a non enterotoxigenic serotype). These solutions were perfused in the small intestine of rats "in vivo" and sodium transport was determined. The comparison among the 3 control solutions revealed that there was always sodium absorption, but there was a difference in the intensity of the transport (SC1 >SC2 and SC3). The serotypes 0111:H-, 026:H-, 0126:H21, and 0142:H2, as well as the serotype 0157:HNT, induced sodium secretion and these values were significantly different in comparison with the 3 control solutions. On the other hand, the serotypes 0128ab:H35 and 0119:H6 induced sodium secretion but the differences were only significant in comparison with the SC1 and SC2, and SC1, respectively. The supernatants of the serotypes investigated provoked an important derangement in sodium transport probably due to the presence of an enterotoxin.

English Abstract↗
PubMedDate not supplied

[Cidade Leonor slum, São Paulo. III. Environmental contamination and natural feeding practice].

In this study the authors report the high rate of environmental contamination to which children living in slum areas are exposed. The authors performed a bacteriologic and parasitologic survey in children with acute diarrhea and in controls. Feeding habits were also recorded. The feeding habits revealed that 176 out of 520 children (33.8%) were never breast fed, whereas the remaining 344 (66.7%) were breast fed for an average of 2.2 months. The parasitologic survey was positive in 89% of the stool samples obtained from 83 children, mostly with multiple infections. The bacteriologic survey revealed the presence of enteropathogens and, in particular, invasive E. coli and Campylobacter in 46.7% of the children with acute diarrhea, and in 32.5% of the children in the control group. These findings allow the authors to state that environmental conditions of slum areas directly predispose to the appearance of the diarrhea-malnutrition binomial.

Brazil↗
PubMedDate not supplied

An Escherichia coli strain that causes diarrhea by invasion of the small intestinal mucosa and induces monosaccharide intolerance.

E. coli can induced diarrhea either by enterotoxin production or by invasion of the colonic mucosa. Here we report a 2/12 year old infant with caute diarrhea induced by E. coli strain, isolated from the jejunal fluid, that had no enterotoxigenic activity but invaded the small intestinal mucosa and induced severe morphological alterations. Total villous atrophy and monosaccharide intolerance occured. After 51 days of hospitalization there was a partial recovery of the small intestinal morphology and the patient could also tolerate disaccharides again.

Acute Disease↗