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Biomedical subjects

L Remvig

Publications and source records attributed to L Remvig.

34 records · Page 2Linked to original sources
PubMed1985

Phorbol myristate acetate--fate and usability in a human interleukin-1 assay.

Phorbol myristate acetate (PMA) has been widely used to produce interleukin-1 (IL-1) from monocytes (Mø) of various species, including man. Supernatants from cultures of human Mø, stimulated with PMA, 2 X 10(-6) M, contained IL-1-like activity, as judged by their high co-mitogenic effect on mitogen-stimulated human T-cells. However, a solution of PMA in culture medium was equally as active as the supernatants. By way of 3H-PMA it was demonstrated that the IL-1-like effect of PMA-induced supernatants was non-dialyzable, was coupled to proteins with a molecular weight greater than 70,000 Dalton and could be dependent mainly upon the PMA content. Thus, previous reports dealing with basic immunological as well as immunopharmacological aspects of IL-1, must be interpreted with caution if the conclusions have been based on results obtained with crude or high molecular weight forms of PMA-induced IL-1. It could be that PMA can be used as an IL-1 inducer. However, it must first be demonstrated that PMA does not interfere with the detection of IL-1 or, if so, that PMA has been removed sufficiently from the test material to avoid interference. Secondly, the specificity of PMA with respect to IL-1 inducing effect must be demonstrated under endotoxin-free culture conditions.

Biological Assay↗
PubMed1985

A standardized human T-lymphocyte proliferation assay for detecting soluble accessory factors from monocytes. II. Interleukin-1 production and detection.

Conditions for production and detection of a monocyte-derived interleukin-1 like accessory factor were investigated. The production of the accessory factor was not increased quantitatively by changes in monocyte concentration or incubation times. Lipopolysaccharides stimulated monocytes to accessory factor production in a broad range of concentrations, and it was active even in concentrations below the often registered endotoxin contamination of commercial culture media. Thus, to avoid unintended monocyte stimulation culture conditions should be endotoxin-free. The accessory factor was heat-labile, had a MW between 10 and 40 K, was co-mitogenic to PHA-stimulated monocyte depleted cells and it did not support the growth of an interleukin-2 dependent cell-line. Thus the factor shares characteristics with and might be identical to interleukin-1. The conditions previously defined for the demonstration of accessory effect of monocytes were also optimal for demonstration of interleukin-1 in supernatants from lipopolysaccharide stimulated monocyte cultures.

Cells, Cultured↗
PubMed1984

Influence of endotoxin on production of leucocyte migration inhibition factor. Part I.

Commercial reagents are often contaminated with endotoxins in concentrations which may affect biological immunoassays. The endotoxin lipopolysaccharide from E. coli 026:B6, at concentrations at and above 5 X 10(-9) g/ml induces mononuclear cells to produce the lymphokine leucocyte migration inhibition factor (LIF). However, lipopolysaccharide at concentrations at or above 5 X 10(-8) inhibit leucocyte migration making it impossible to measure the effect of LIF. Standardization of endotoxin content during production and assay of LIF is needed, in order to reduce the variability of the results.

Adult↗
PubMed1984

A standardized human T-lymphocyte proliferation assay for detecting soluble accessory factors from monocytes. I. Monocyte and monokine requirements.

Monocyte (M phi-) depleted human peripheral blood mononuclear cells exhibit reduced capacity to proliferate, when stimulated with low doses of phytohaemagglutinin (PHA). Stimulation with higher doses of the mitogen or addition of graded numbers of autologous M phi's potentiated the response proportionally, and 10% M phi's restored the response completely. Addition of only 0.1% M phi's was needed for potentiating the response significantly. Pretreatment of M phi's with Actinomycin-D or heating impaired the accessory effect, whereas x-irradiation had no effect. The presence of M phi's did not influence the viability of the proliferating lymphocytes. Crude supernatants from M phi-cultures, even at very low concentrations, partly restored the PHA response, as did preparations of highly purified human Interleukin-1.

Cell Communication↗
PubMed1982

Con A induced suppressor test: an evaluation of the experimental conditions.

The suppressor activity measured by the Con A mixed lymphocyte culture assay exhibited a pronounced dependence on the technical performance as well as a marked inter-individual variation. The activity exerted by the induced suppressor cells depended on the Con A concentration in the induction phase, and on the mitotic activity in the responder system. The patterns of suppressor activities obtained in an allogeneic and an autologous suppressor test system were identical. When calculating the suppressor activity it is suggested, that one uses the total activities of the test system, without any reduction corresponding to the activity of the mixed lymphocyte culture. It is suggested that the suppressor activity is expressed by a "suppressor profile" to make the results more applicable and the interpretation of different works meaningful.

Adolescent↗
PubMed1980

The effect of antacid on gastric and duodenal bulb acidity.

A small glass pH electrode was positioned in the duodenal bulb and connected to a digital pH meter. The pH registered every half second was stored on a minidiskette with a capacity of 32.400 pH data on a single mini-disc. A microcomputer controlled electrode calibration, data collection, formating and storage. Four patients with duodenal ulcer were given a liquid test meal, and one hour later when gastric and duodenal acidity had reached high levels they were given 10 ml of antacid. From the stored pH data the mean pH and the mean hydrogen ion concentration was calculated. During the 10 min. period before antacid was given gastric pH was on average 1.5 and the duodenal pH on average 4 with a CH+ of 2.500 mumol/. Ingestion of antacid reduced gastric acidity to around pH 3, and the duodenal pH to 6 with a CH+ of 250 mumol/l. This effect of antacid was observed in all subjects, but the degree and the duration of the effect varied very much between the individuals.

Antacids↗
PubMedDate not supplied

Supervised endurance exercise training compared to home training after first lumbar diskectomy: a clinical trial.

OBJECTIVE: We performed a randomised trial to evaluate if intensive supervised training of the back should be offered to all patients after a first lumbar diskectomy. METHODS: Forty consecutive patients were, after a first lumbar diskectomy, randomly allocated to 2 groups undergoing "supervised training" twice a week for 3 months in an outpatient clinic or "home training" after 2 hours of instruction. RESULTS: The two rehabilitation models both showed a significant effect on spinal mobility, isokinetic trunk flexion strength, isokinetic trunk extension strength and daily function. These improvements were unchanged at follow up 3 months later. The pain score remained unchanged, however, throughout the trial in both groups. No differences in effect between the two rehabilitation models could be found for any of the assessed parameters. Thirteen patients did not complete the trial, including 9 from the supervised endurance trained group, mainly because of increased pain and reprolaps (n = 4). Four patients dropped out of the home trained group, only one because of increased pain. The differences in drop-out rate and training side effects were, however, not statistically significant. CONCLUSION: We conclude that it is not worthwhile to implement 3 months of supervised intensive endurance training as opposed to home training in all cases of first lumbar diskectomy, although a beneficial effect and better compliance might be found for a selected group of such patients.

Adult↗