Biomedical subjects
L Rioja
Publications and source records attributed to L Rioja.
Serotypes, toxins and antibiotic resistance of Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Spain.
One hundred and ten Escherichia coli strains isolated from diarrhoeic and healthy rabbits from 50 Spanish commercial farms were serotyped and investigated for production of enterotoxins (LT and STa), verotoxins (VT), cytotoxic necrotizing factors (CNF1 and CNF2), alpha-haemolysin (Hly) and enterohaemolysin (EntHly), for necrotic and lethal activities and for antibiotic resistance. Six serogroups (O2, O26, O49, O92, O103 and O128) accounted for 81% (67 of 83) and 26% (7 of 27) respectively of E. coli strains isolated from diarrhoeic and healthy rabbits (P < 0.001). The most common serotypes found among E. coli strains associated with diarrhoeic rabbits in order of frequency were: O103:K-:H2, O49:K?:H2, O26:K-:H-, O26:K-:H11, O128:K?:H-, O92:K-:H2 and O2:K5:H6. E. coli strains belonging to the same serotype but from different farms usually showed a distint antibiotics resistance pattern. Only one strain, of serotype O2:K5:H6 was toxigenic (CNF1+, Hly+, necrotic and lethal).
[Treatment of metastatic prostatic cancer with monthly injections of leuprolide acetate depot].
Seventy-two patients were admitted in a multicentre trial with the purpose of assessing the clinical efficacy and safety of the hormonal control and tolerance of leuprolide acetate in a once-a-month depot injection formulation for the treatment of disseminated prostate cancer. During a 1-year follow-up, there were ten withdrawals for different reasons. At baseline and at 6 months of treatment the following parameters were evaluated: clinical examination, routine blood analysis, PAP, PSA, LH and testosterone, as well as bone scan. LH and testosterone determinations were repeated at 2, 4, 8, 12, 16, 20 and 24 weeks. Testosterone reached castration levels within the second week and was maintained until the end of the study. In agreement with the NPCP criteria, 65 patients were assessed as: complete response 3%, partial response 40%, disease stabilization 36%, and progression 21%. In summary, a once-a-month injection of leuprolide acetate offers a safe and effective alternative to surgical castration.
Bone disease in children with homozygous beta-thalassemia.
The histological features of thalassemic bone are imperfectly known, and the roles of bone marrow hyperactivity, iron overload or vitamin D deficiency in the pathogenesis of the disease are not clearly identified. In this study we examined iliac crest biopsies from 17 transfusion-dependent children with homozygous beta-thalassemia and severe radiological skeletal thalassemic changes, including widening of medullary spaces and osteoporosis. Rachitic lesions were not observed. Serum ferritin concentrations were increased in all but one subject. Iron deposits were histochemically detected in bone marrow, at the marrow-bone interface, along cement lines and mineralizing perimeters. Minor changes were present in trabecular bone, and osteomalacia was absent. By contrast, cortical bone exhibited severe changes including fissures and focal mineralization defects. Plasma 25-hydroxyvitamin D (25(OH)D) concentrations measured during the winter (December-May, 6.5 +/- 4.9 ng/ml, mean +/- SD, n = 6) and during the summer (June-November, 13.8 +/- 8.4 ng/ml, n = 9) did not differ from those of age-matched children living in the same country. Seven patients had moderate hypocalcemia but no biological signs suggestive of vitamin D deficiency: all had normal alkaline phosphatase activity, normal or slightly elevated plasma phosphate, only two had low plasma 25(OH)D concentrations and two others supranormal values of plasma immunoreactive parathyroid hormone. These results show that iron overload and vitamin D deficiency do not seem to play an important role in the pathogenesis of thalassemic bone disease, which is characterized by cortical lesions probably related to marrow hyperactivity.
[Surgical aspects of coralliform lithiasis. Our experience].
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