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Biomedical subjects

L Rodriguez

Publications and source records attributed to L Rodriguez.

At least 91 records · Page 5Linked to original sources
PubMed1980

Metabolic relationship between invertase and acid phosphatase isoenzymes in Saccharomyces cerevisiae.

Repressed cells of Saccharomyces cerevisiae, subjected to inhibition of both RNA and protein synthesis, showed a pattern of membrane-bound and cytosol acid phosphatase to the external enzyme which seemed to be linked through a precursor-product relationship. Gel exclusion chromatography did not indicate clear differences between the isoenzymes. Moreover, centrifugation experiments in CsCl and precipitation with concanavalin A suggested that there were no acid phosphatase molecules devoid of carbohydrate. Membrane-bound invertase displayed a molecular weight and a carbohydrate to protein ratio smaller than those of the exocellular enzyme. The values of molecular weight and buoyant density of the membrane-bound enzyme were closer to those found for the cytosol invertase. The stability of the level of the soluble invertase detected in the cytoplasm under derepression conditions, or after RNA or protein synthesis inhibition was found to be only apparent and represented the result of an equilibrium between synthesis and degradation.

Acid Phosphatase↗
PubMed1979

Rupture of the inferior vena cava during open-heart surgery. Aetiology and surgical management.

Bleeding during open-heart surgery is a not unusual complication. Rupture of the inferior vena cava during dissection of the heart from adhesions from a previous operation for mitral disease or congenital palliative surgery is a serious and sometimes fatal complication. Its treatment is a challenge even to the most experienced heart surgeon. In two of our three patients, vena cava rupture occurred during open-heart surgery for correction of mitral and aortic valvular disease. In one patient, congenital infundibular stricture of the right ventricle, associated with tricuspid valve insufficiency, was present. In this report, some relevant data were presented from each case and the aetiology of the rupture is discussed.

Adult↗
PubMed1979

Successful replacement of the aortic valve, mitral valve and ascending aorta with re-implantation of the coronary arteries.

A 63-year-old man was operated upon with replacement of the aortic valve, mitral valve and ascending aorta with re-implantation of the coronary arteries. A composite graft with a 27 mm Björk-Shiley tilting disc prosthesis was inserted with 3 continuous Prolene sutures in the aortic position. The coronary arteries were thereafter re-implanted in holes made in the graft. A 31 mm Björk-Shiley valve was sutured in the mitral position with about 20 isolated mattress sutures of Ti-Cron. Selective myocardial hypothermia was instituted with Ringer's solution of 4 degrees C. The total perfusion time was 185 min compared with 240 and 275 min in two previously reported, similar cases. We consider that the use of cardioplegia, a composite graft and continuous sutures makes it possible to reduce the operation time in these complex cases. Our patient is in good condition 9 months postoperatively.

Aortic Aneurysm↗
PubMed1978

Regulation of acid phosphatase synthesis in Saccharomyces cerevisiae.

In Saccharomyces cerevisiae-136ts (Hutchison, H.T., Hartwell, L.H. and McLaughlin, C.S. (1969) J. Bacteriol. 99, 807--814) derepressed acid phosphatase was almost exclusively located outside the permeability barrier. Only a minor part of the activity was associated with the protoplasts; about half of it (48%) in the soluble fraction, the rest bound to the internal (45%) and plasma (7%) membranes. The activity found in the membranes of derepressed cells decreased by 30--40% after addition of inorganic phosphate or cycloheximide suggesting that this activity is the precursor of the external enzyme. The alkaline phosphatase activity level could not be modified by changes in the concentration of inorganic phosphate. Acid phosphatase was not synthesized if the cells were transferred to a low phosphate medium at the moment of incubation at 37 degrees C or in the presence of cycloheximide at 23 degrees C. The data suggested that enzyme formation is the result of the transcription and translation of a specific gene(s) and not the activation of a proenzyme. Inorganic phosphate did not inhibit the translation of mRNA though it may act at the level of the transcription.

Acid Phosphatase↗
PubMed1978

Human tumor-lymphocyte interaction in vitro. III. T lymphocytes in autologous tumor stimulation (ATS).

The T-cell nature of the responding cells in autologous tumor stimulation (ATS) was proven by (1) E-rosette formation of lymphocytes attached to tumor cells during the early period of co-cultivation; (2) blast-transformed E-rosetting cells detectable at the end of the test period (day 6) and (3) the reactivity of T-enriched lymphocyte subset and unresponsiveness of T-depleted fractions. Preparation of tumor suspensions devoid of lymphocytes made it possible to carry out cross-tests between patients. Blastogenesis with allogeneic tumors was rare. The restricted autologous nature of the reaction is a strong indication for genuine tumor-relatedness.

Adenocarcinoma↗
PubMed1977

Two-stage replacement of tricuspid valve in active endocarditis.

One month after a 21-year-old female drug addict with tricuspid staphylococcal endocarditis seemed to have been cured by antibiotic therapy she relapsed. Further antibiotic treatment for one month failed to control the infection. Tricuspidectomy was when performed and the infection was eliminated. A valve prosthesis was inserted 3 months later. She recovered and is now fully restored to health. We recommend this two-stage surgical technique in similar cases.

Adult↗
PubMed1977

Left ventricular rupture as a complication of mitral valve replacement.

We are not the first to attempt repair of left ventricular rupture complicating mitral valve replacement, as 18 analogous cases have been reported earlier in the literature. Our series comprises 8 patients, 5 of who, survived. A review of these 26 cases provided several facts of interest to surgeons dealing with this complication. The rupture occurred either as a laceration in the posterior atrioventricular groove (type I, 16 of 26 patients) or as a perforation of the midportion of the left ventricle (type II, 10 of 26 patients). Intraoperative rupture (17 of 26 patients) was usually detected on termination of bypass, whereas delayed rupture (9 of 26 patients) occurred after chest closure or in the recovery room. The morality rate was about 50 per cent for the intraoperative type, and no patient survived a delayed rupture. The prognosis appeared to be most in intraoperative type II lesions. The main factors affecting the prognosis were (1) instant reinstitution of extracorporeal circulations and (2) avoidance of the circumflex coronary artery during repair of type I lesions located close to the anterolateral mitral commissure. Attempts to suture a ventricular rupture on the pressure-loaded, beating heart were always unsuccessful and frequently extended the laceration. Patients with the delayed type of rupture died of hemorrhage before they could again be placed on bypass. It may be preferable to reopen the lefr atrium in order to repair a type I laceration. In type II perforations, direct repair with buttressed sutures should be attempted from the exterior of the heart. The angulated metal cannula for drainage of the left ventricle was identified as a possible, but not previously reported, cause of myocardial perforation. It is hoped that the use of a soft cannula or a metal cannula with an angle of 60 degrees instead of 90 degrees will reduce the incidence of this complication.

Female↗
PubMed1977

Percutaneous fixation of supracondylar fractures of the humerus in children.

Of 1,118 supracondylar fractures of the humerus in children treated over a six-year period, 189 are described with a follow-up of more than two years. These patients were treated with closed reduction and percutaneous fixation of the fragments with two wires. Eighty-five per cent of the final results were satisfactory.

Child↗
PubMed1975

Modification by calcium dobesilate of histamine effects on capillary ultrastructure.

The ultrastructure of blood capillaries and venules are studied in rat skin. After i.v. injection of histamine the luminal surface of the endothelial cells show protrusions of variable size. Numerous gaps have been found in the capillary wall, specially in the venules. These alterations are not observed in the animals that were treated with calcium dobesilate before the administration of histamine. In these cases the capillary structure are indistinguishable of the controls. The possible effects of the histamine and calcium dobesilate on the cell coat and cell junctions of the endothelial capillary cells are discussed.

Animals↗
PubMed1975

Surgical implications of right aortic arch with isolation of left subclavian artery.

Few cases have been reported of the combination of right aortic arch with isolation of the left subclavian artery in childhood because of the absence of symptoms from this pairing of defects and the difficulty of its recognition by routine examination. All the reports have been associated with congenital heart disease. We report 4 cases. In one of them, the diagnosis was made during an attempt to create a left Blalock-Taussig anastomosis and the technique was modified. The diagnosis can be made before operation with an aortogram. This procedure should be carried out to show the aortic arch branches in cases where palliative surgery is contemplated.

Angiography↗