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L Romualdo

Publications and source records attributed to L Romualdo.

4 recordsLinked to original sources
PubMed1996

Cupressaceae pollinosis in the Madrid area.

BACKGROUND: There is no previous report of Cupressaceae pollinosis in the Madrid area. OBJECTIVE: To ascertain the presence of Cupressaceae pollinosis in the Madrid area. METHODS: Thirty-six patients were studied who had winter pollinosis symptoms and a positive skin test to C. arizonica. They were skin tested with C. sempervirens (Abelló, Madrid), C. arizonica (in-house extract) and other common inhalant allergens (Abelló). Total IgE was measured and specific IgE to C. arizonica, C. sempervirens and J. sabinoides was determined. RESULTS: Six of the patients were only sensitized to Cupressaceae pollens, whereas 30 patients had also sensitivity to other pollens (oleaceae 28, plantain 27, grasses 25, chenopodium 25, compositae 23, Parietaria 9) and 18 patients were also sensitized to other inhalant allergens (animals 17, mites 4, moulds 2). CONCLUSION: These results confirm the existence of allergy to Cupressaceae pollens in Madrid (Spain).

Adolescent↗
PubMed1995

Cross-reactivity of IgE-binding components between boiled Atlantic shrimp and German cockroach.

IgE-antibody reactivity to boiled Atlantic shrimp (Pandalus borealis) and German cockroach (Blattella germanica) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP-FEIA System, Pharmacia, Sweden). IgE serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. A positive anticockroach IgE was very rare in the absence of detectable antishrimp IgE (five of 89 sera). Linear regression analysis on antishrimp and anti-German cockroach IgE levels-log plot revealed a correlation coefficient (r) of 0.73. Inhibition experiments showed that boiled Atlantic shrimp extract inhibited CAP with German cockroach, and vice versa. Immunoblotting showed the strongest IgE binding for both allergenic extracts between 30 and 43 kDa. By blot inhibition, the binding capacity of German cockroach was totally abolished by Atlantic shrimp extract, while German cockroach extract only partially IgE binding to Atlantic shrimp. Cross-reactivity exists between shrimp, an important food allergen, and German cockroach, which has an increasing role in allergic asthma. It could be important to determine the clinical significance of cross-allergy to both allergens, in which exposures occur in different ways.

Adolescent↗