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Biomedical subjects

L Rudin

Publications and source records attributed to L Rudin.

9 recordsLinked to original sources
PubMed1987

Multicentre evaluation of a direct coagglutination test for group A streptococci.

A coagglutination test for detection of group A streptococci in throat samples was evaluated in a multicentre study and found to be about 95% sensitive when applied to swabs taken from symptomatic patients which yielded more than 100 colonies per plate on culture. The sensitivity of the test dropped significantly when it was applied to swabs giving fewer than 100 colonies on culture. The specificity of the test was high regardless of colony count on conventional media.

Acute Disease↗
PubMed1976

Ureteroureterostomy and unilateral nephrostomy. Alternate rapid method of diversion.

Ureteroureterostomy with a Silastic nephrostomy has been utilized in 3 patients as a temporary diversion. It is a short procedure which is performed transabdominally and is well tolerated. It may be indicated in selected patients who will not be able to handle an appliance and cannot tolerate major surgery. This is not a panacea for urinary diversion, but in selected cases it may be applicable.

Aged↗
PubMed1975

Thymineless bacteriophage induction in Staphylococcus aureus. I. High-frequency transduction with lysates containing a bacteriophage related to bacteriophage phi 11.

A thymine-requiring mutant of Staphylococcus aureus, strain 8325 (PI258)thy, undergoes prophage induction and lysis after thymine starvation. Four different phages were isolated from the lysate in low titers, among which was a phage designated phi 14, which differs from phage phi 11 in its immunity locus. The thymineless induced lysates of strain 8325(PI258)thy transduce the penicillinase plasmid at high frequency (10(-1), whereas transduction of chromosomal markers is inefficient. A plasmic-cured derivative of strain 8325(PI258)thy is also lysed by thymine starvation and be used for high-frequency transduction of other plasmids. Reconstitution of a strain of S. aureus that responds to thymine starvation was only partially successful, but this system can effectively be used to transduce plasmids or plasmid derivatives.

Drug Resistance, Microbial↗
PubMed1975

Thymineless bacteriophage induction in Staphylococcus aureus. II. Specific transduction of constitutive and inducible erythromycin resistance.

Specific transduction of inducible (eroA) and constitutive (eroB) erythromycin resistance is mediated by thymineless induced lysates from derivatives of Staphylococcus aureus strain 8325(N)thy. Both loci can coexist in the same cell but segregate by transduction or transformation. The gene(s) is probably integrated in the recipient chromosome and excised at thymine starvation.

Chromosomes, Bacterial↗
PubMed1974

Genital sarcoidosis.

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Adrenal Cortex Hormones↗
PubMed1974

Factors affecting competence for transformation in Staphylococcus aureus.

A chemically defined medium has been developed for isolation of amino acid-requiring mutants of Staphylococcus aureus strain 8325, and for use as a selective medium in transformation assays. Variables affecting transformation of both plasmid and chromosomal markers have been studied. The optimal pH and temperature for transformation are 6.75 to 7.0 and 30 C, respectively. Ca ions are required for transformation, and only cells lysogenic for the phage phi11 can be transformed. Superinfection of competent cells with phi11 does not increase the transformation frequency. Maximal number of transformants is obtained after 20 min of contact between cells and deoxyribonucleic acid. The transformation frequencies for the plasmid marker erythromycin resistance (ero) and the chromosomal markers trp, thy, and cyt are of the same order of magnitude, whereas the frequency for the chromosomal marker tyr is approximately one order of magnitude lower.

Barium↗