PubMed Health⌕ Search

Biomedical subjects

L Rutberg

Publications and source records attributed to L Rutberg.

62 records · Page 4Linked to original sources
PubMed1969

Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105.

Bacteriophage phi105 is a temperate phage for the transformable Bacillus subtilis 168. The infectivity of deoxyribonucleic acid (DNA) extracted from mature phi105 phage particles, from bacteria lysogenic for phi105 (prophage DNA), and from induced lysogenic bacteria (vegetative DNA) was examined in the B. subtilis transformation system. About one infectious center was formed per 10(8) mature DNA molecules added to competent cells, but single markers could be rescued from mature DNA by a superinfecting phage at a 10(3)- to 10(4)-fold higher frequency. Single markers in mature DNA were inactivated at an exponential rate after uptake by a competent cell. Prophage and vegetative DNA gave about one infectious center per 10(3) molecules added to competent cells. Infectious prophage DNA entered competent cells as a single molecule; it gave a majority of lytic responses. Single markers in sheared prophage DNA were inactivated at the same rate as markers in mature DNA. Prophage DNA was dependent on the bacterial rec-1 function for its infectivity, whereas vegetative DNA was not. The mechanism of transfection of B. subtilis with viral DNA is discussed, and a model for transfection with phi105 DNA is proposed.

Bacillus subtilis↗
PubMed1969

Characterization of Temperate Bacillus Bacteriophage phi105.

Temperate Bacillus phage phi105 is serologically unrelated to previously described virulent Bacillus phages. Phage phi105 is incapable of generalized transduction. Prophage phi105 is inducible with mitomycin C. Phage phi105 contains double-stranded deoxyribonucleic acid (DNA) with a molecular weight of about 25 x 10(7) as determined by band sedimentation and electron microscopy. The per cent guanine plus cytosine of phi105 DNA is 43.5 as determined by buoyant density in CsCl and by thermal denaturation. Phage phi105 DNA may contain complementary single-stranded ends.

Journal Article↗
PubMed1966

Bacteriophage-induced functions in Escherichia coli K (lambda) infected with rII mutants of bacteriophage T4.

Rutberg, Blanka (Karolinska Institutet, Stockholm, Sweden), and Lars Rutberg. Bacteriophage-induced functions in Escherichia coli K(lambda) infected with rII mutants of bacteriophage T4. J. Bacteriol. 91:76-80. 1966.-When Escherichia coli K(lambda) was infected with rII mutants of phage T4, deoxycytidine triphosphatase, one of the phage-induced early enzymes, was produced at initially the same rate as in r(+)-infected cells. Deoxyribonuclease activity was one-third to one-half of that of r(+)-infected cells. This lower deoxyribonuclease activity was observed also in other hosts or when infection was made with rI or rIII mutants. Presence of chloramphenicol did not allow a continued synthesis of phage deoxyribonucleic acid in rII-infected K(lambda). No phage lysozyme was detected nor was any antiphage serum-blocking antigen found in rII-infected K(lambda). It is suggested that the rII gene is of significance for the expression of phage-induced late functions in the host K(lambda).

Antigens↗
PubMed1965

Role of superinfecting phage in lysis inhibition with phage T4 in Escherichia coli.

Rutberg, Blanka (Karolinska Institutet, Stockholm, Sweden), and Lars Rutberg. Role of superinfecting phage in lysis inhibition with phage T4 in Escherichia coli. J. Bacteriol. 90:891-894. 1965.-The ability of bacteriophage T4 to induce lysis inhibition upon superinfection was investigated after various treatments of the phage. This ability was found not to be a property of the external protein part of the phage, nor was it dependent on the functional and possibly structural integrity of the phage genetic material.

Coliphages↗