Biomedical subjects
L S Iakovleva
Publications and source records attributed to L S Iakovleva.
[Latent ectromelia virus carrier state in mice of BALB-c line].
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[Isolation and passaging of Marek's disease virus in cell cultures].
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[A study of the properties of the Kekava strain of Marek's disease virus in experiments on chickens].
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[Effect of cream-butter and oil on the course of liver diseases in children].
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[Vaccinating properties of the Kekava 55 strain of attenuated Marek's disease virus].
Two attenuated strains of oncogenic herpes virus, Marek's disease virus, were obtained: one by cultivation in chick fibroblast cultures at 37 degrees C, the other at 41 degrees C, i. e. at chicken body temperature. Both attenuated strains throughout a large number of passages retain high vaccination efficacy, on the average 76% and 77.3%, respectively. The virus attenuated at 41 degrees C is superior to that attenuated at 37 degrees C in higher reproduction rate in vitro and, possibly, in a greater range of passages during which good vaccinating properties are retained. Unlike turkey herpes virus, both attenuated strains reduce the spread of the infection markedly.
[Comparative antigenic characteristics of 2 Marek's disease virus variants and of herpesvirus of turkeys isolated in the USSR].
The antigens induced by pathogenic (55) and nonpathogenic (83) variants of Marek's disease virus, the Kekava strain (MDV-Kekava) and herpesvirus of turkeys, the M-24-72 strain (HVT-M24-72) were tested in the agar gel precipitation test. The antigens were prepared from feather follicles (FF) of the infected chickens, from the infected cultures of chick embryo fibroblasts (CEF) and from the culture fluid (CF) of these culttures. In the FF and CEF cultures infected with MDV-Kekava-83, the synthesis of the antigens was 6-10 times as low as in the FF and CEF cultures infected with MDV-Kekava-55. Both variants of MDV-Kekava retained their capacity for antigen synthesis in the CF up to 84 passages. Three antigens or three groups of antigens were found in the preparations examined: (1) strong common antigens in the CF and FF preparations; (2) weak common antigens in cell preparations and CF; (3) strong antigen(s) in cell preparations not identical to the CF and FF antigens. In CEF culture preparations infected with MDV-M-24-72, using sera from chickens with Marek's disease an antigen was detected partially identical to the weak antigen of cell preparations and cross-reacting with strong antigens of the FF, CF and cell antigens of MDV. No antigen was found in concentrates of CF from the CEF cultures infected with MDV-M-24-72 with the sera used. It is suggested that the CF and FF MDV antigens are virus antigens, while antigens of the cell preparations of this strain are autoantigens or embryonal antigens. Data are presented on relationship between the MDV-Kekava antigens with the MDV-HPRS-16 antigen.
[Properties of pigeon sera containing complement-fixing antibodies to the gs-antigen of the avian leukosis-sarcoma complex].
Pigeons bearing tumors caused by the Schmidt-Ruppin strain of Rous sarcoma virus were used for withdrawing sera containing complement-fixing (CF) antibody to the gs-antigen of avian leukosis-sarcoma complex. In the course of this study it was found that some of these sera, while having a high titer of CF antibody to the gs-antigen of the tumor tissue, did not detect this antigen in chicken embryonal tissue and feather follicles. It is suggested that these sera distinguish different components of gs-antigen in the tumor tissue on the one hand and in the embryonal tissue and feather follicles on the other. There was a correlation in the detection or lack of gs-antigen in preparations of feather follicles and embryonal tissue with all the sera examined. Feather follicles may serve as a convenient source of gs-antigen in practical work.
[Intravital diagnosis of chicken infection with Marek disease virus by a modified method of detecting virus-specific antigen in feather follicle epithelium].
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[Features of adult T-cell leukemia virus reproduction in B-cell lines and it's interaction with the Epstein-Barr herpesvirus].
Interaction between human T-cell leukemia virus (HTLV) with B cells and Epstein-Barr virus (EBV) was studied by immunoblotting, immunofluorescence, virus isolation in permissive T-cell cultures, and polymerase chain reaction (PCR). HTLV-1 in vitro infects the B-cell cultures containing EBV but not EBV-negative cell lines. Productive infection of EBV+ B cells was associated with syncytium formation which led to the elimination of HTLV-1 producing cells. However, the remaining B-cell population contained gag, pol, and pX--the "silent" provirus sequences. HTLV-1 infection of B cells altered the expression of some latent proteins of EBV (EBNA-1, EBNA-2, EBNA-5, and LMP). The changes were represented by increase of molecular weight and/or appearance of additional proteins and were individual for each cell line. Alteration of EBV protein expression may change the functional activity of these proteins, but this hypothesis is to be tested.