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L S Kholodna

Publications and source records attributed to L S Kholodna.

10 recordsLinked to original sources
PubMed2006

[Effects of Staphylococcus aureus cell-bound protein A on adenosine triphosphate and nitric oxide inhibitory actions in smooth muscles].

Cell-bound protein A (CBPA), an immune-active substance of Staphylococcus aureus was ascertained to depolarize membrane of taenia coli smooth muscle (SM) cells, depress ATP inhibiting action (or uridinetriphosphate (UTP)) and sodium nitroprusside (SNP). ATP or UTP-induced membrane hyperpolarization increased during first minutes of CBPA exposure. Bacterial substance enhanced and then inhibited fast component of nicotine-induced relaxation of histamine-activated smooth muscles. This enhancement was inhibited by N(omega)-nitro-Larginine, a NO-syntase blocker. CBPA decreased ATP inhibiting action upon histamine-induced contraction, but enhanced cholinergic SM excitation. All these processes are reversible.

Adenosine Triphosphate↗
PubMed2002

[Transfer factor modulates inhibitory action of neurotransmitters on intestinal smooth muscles].

It has been shown that Transfer factor (TF) to Staphilococcus aureus antigens blocked ATP-induced component of the inhibitory junction potential in taenia coli smooth muscle from guinea-pig, and converted an inhibitory action of exogenous ATP into an exciting one (instead of the hyperpolarization of the smooth muscle, TF induced its depolarization). TF at 10-6 mg/ml converted the relaxing effects of sodium nitroprusside (nitric oxide donor) into exciting ones in smooth muscle strips. Higher concentrations (10-5-10-3 mg/ml) of TF slightly amplified the relaxing effect of sodium nitroprusside. Both a- and b-adrenergic activation in taenia coli smooth muscle were not sensitive to that agent.

Adenosine Triphosphate↗
PubMed2000

[The immune reactivity transfer factor as a modulator of lymphocyte functional activity in rats].

The transplantation of the thyroid tissue is one of the perspective methods for rehabilitation of thyroid gland functional disorders that appear due to the influence of insufficient environmental conditions on organism. By means of micromethod of lymphocyte blast transformation reaction on the base of [3H]-thymidine shift the functional activity of the Wistar rat's splenocytes was studied in case of radiation induced hypothyroidism with or without xenotransplantation of newborn pig thyroid gland organ culture. It was found that the level of thyroxine and triiodothyronine significantly decreased in serum of irradiated animals, the lymphocyte proliferation level was also reduced (by means of radioiodine introduction in dose of 8,325 MBk/mmole). Application of thyroid gland tissue xenotransplantate in this model of hypothyroidism helped to achieve the increasing of thyroid hormones levels in serum and rehabilitation of lymphocytes functional activity. The opportunities for correction of immunological disorders with the help of transfer factor of immune reactivity preparates were investigated. Transfer factor--is a low-molecular weight leukocyte extract (J 10 kD) with immunomodulating activities. This preparates activated the proliferation of splenocytes from animals with hypothyroidism and animals with hypothyroidism after xenotransplantation.

Adjuvants, Immunologic↗
PubMed1997

[Human specific transfer factor to Staphylococcus aureus antigens].

Immunobiological properties of human specific transfer factor (TF) to Staphylococcus aureus antigens were studied. It is shown that this TF activated human leucocytes in vitro as well as in vivo. Antigen specificity of TF's immunomodulating effects is also shown. In vitro we used leucocyte migration inhibition test (IML), macrophage inhibition test (MPI) and rosette formation (E-ros). For testing in vivo we used delayed type hypersensitivity (DTH) skin tests.

Animals↗
PubMed1996

[The isolation of a transfer factor of the delayed-hypersensitivity type to the antigenic substances of Staphylococcus aureus in guinea pigs].

Immunoactivating effects of guinea pig's staphylococcal Transfer factor (TF) were studied in homologous and heterologous (human leukocytes) systems. In vivo (skin tests) and in vitro (Inhibition of leukocytes migration) tests showed that our TF is able to activate intact as well as sensibilized cells. Antigen-specific properties of TF also were showed.

Animals↗
PubMedDate not supplied

[Mitogenic effects of staphylococcal antigenic substances on irradiated lymphocytes from Wistar rats].

Proliferation activity of lymphocytes from rats was studied using a micromethod of blasttransformation reaction after X-ray irradiation (absorbed doses for animals were 0.5 and 1 Gy). Staphylococcus protein A and peptidoglycan stimulated the proliferation of irradiated lymphocytes. It was assumed that staphylococcus antigen substances held radiation protective effects. Staphylococcus protein A may be used for stabilization of lymphopoesis and functional activity of humoral and cellular immunity in irradiated animals due to T- and B-mitogenic properties.

Animals↗
PubMedDate not supplied

[Effect of staphylococcus active substances on ATPase activity of smooth muscle actomyosin and myosin].

The effect of staphylococcus active substances--protein A (PA) and peptidoglican (PG) at concentrations 10(-6)-10(-2) mg/ml on the ATPase activity of pig stomach natural actomyosin and myosin was studied. It was shown that PA and PG at direct contact with smooth muscle contractile proteins caused the activation and inhibition of ATPase activity, respectively. On the basis of this investigation it was assumed that staphylococcal active substances were able to modify of the ATPase activity smooth muscle contractile proteins perhaps via direct action on the myosin molecule, which could be accompanied by conformational changes of the active center of myosin ATPase.

Animals↗
PubMedDate not supplied

[The biological activity of the transfer factor induced by bacterial antigens].

Today's statement of transfer factor, an immunostimulator derived from leukocytes which enhances antiinfectious immunity, is observed in the review. Basic biological, physical and chemical characteristics of the transfer factor, its possible action mechanisms, and laboratory and clinical methods of use to cure infectious fungal (Candida, Coccidium), invasive (schistosomiasis, leishmaniasis, cryptosporidiosis), viral (varicella zoster, ophthalmic herpes, Herpes simplex types 1 and 2, H. zoster, H. simplex ceratitis, genital herpes, human herpes virus type 6, postherpetic neuritis, hepatitis B, AIDS), and bacterial infections (Mycobacterium leprae, M. tuberculosis, M. fortuitum, Salmonella cholerae suis, S. dublin, S. Virchov, Brucella abortus, Actinobacillus pleuropneumoniae, bacterial sepsis, Staphylococcus) are described.

Animals↗
PubMedDate not supplied

[DNA synthesis in lymphocytes of irradiated animals as affected by mitogens and Staphylococcal antigens].

Proliferation activity of lymphocytes from rats was studied using micromethod of blast transformation reaction after exposure of X-ray irradiation (absorbed doses for animals are 0.5 and 1 Gr). The reducing of the functional activity of lymphocytes was found. There was difference in lymphocyte response induced by standard mitogens (PHA, ConA, LPS) and antigens of Staphylococcus protein A and peptidoglycan. The irradiation protective effects of Staphylococcus substances were found.

Animals↗