PubMed Health⌕ Search

Biomedical subjects

L S Kind

Publications and source records attributed to L S Kind.

At least 19 recordsLinked to original sources
PubMed1983

Homologous adoptive cutaneous anaphylaxis (HoACA)--a method which detects IgG antibody in the mouse at the cellular level.

We describe a new method, homologous adoptive cutaneous anaphylaxis (HoACA). HoACA will detect IgG at the cellular level if mice are immunized in such a manner that no IgE is synthesized. HoACA could be utilized for the same purposes as a previously described method, heterologous adoptive cutaneous anaphylaxis (HACA). HACA detects IgE at the cellular level and has been used primarily to study the establishment of tolerance. It has also been used for localizing the site of IgE formation and for determining the effect of drugs on IgE formation.

Animals↗
PubMed1982

Enhanced IgE and IgG anti-melittin antibody formation induced by heparin-Melittin complexes in mice.

It is known that heparin which is strongly anionic will precipitate in vitro with cationic proteins such as melittin, the principal constituent of bee venom. Our experiments indicate that mice immunized with complexes of melittin and heparin produced greater amounts of IgE and IgG antibody (to this protein) than did mice immunized with melittin alone. It is possible that heparin released from mast cells following a bee sting could complex with melittin and enhance the allergenic properties of this molecule.

Animals↗
PubMed1981

Antigenic, adjuvant and permeability enhancing properties of melittin in mice.

Melittin, a mast cell lytic agent which comprises 50% of the protein content of bee venom, may be responsible for some of the severe reactions produced by bee stings in allergic and non-allergic individuals. We therefore investigated some of the biologic properties of this molecule in mice and found that repeated intradermal injections of melittin without adjuvant induced IgE formation in mice. In addition, melittin as well as another surfactant, saponin, were potent adjuvants for IgE formation in mice when mixed with ovalbumin (Ov). Titres of 1280 and 2560 to ovalbumin were produced in CBA mice after two injections of melittin and Ov or saponin and Ov. Melittin was also shown to have an enhancing effect on the vascular permeability induced by an antigen antibody interaction in mouse skin. Evan's blue dye was injected intravenously into ovalbumin-sensitized mice which had been injected intradermally with ovalbumin alone, melittin alone or a mixture of melittin and ovalbumin. The amount of local extravasation of dye was significantly greater in the area injected with the melittin and ovalbumin mixture than the amount of dye obtained from the skin site injected with melittin alone plus that extracted from the site injected with ovalbumin alone.

Adjuvants, Immunologic↗
PubMed1979

Reduction of contact sensitivity reactions to oxazolone in mite-infested mice.

Oxazolone-sensitized mite-infested (SWR-M) and mite-free (SWR-J) mice were challenged with oxazolone on the skin of the neck and shoulder. The migration of radioactively labeled cells to the site of contact sensitivity reaction to oxazolone was significantly less in SWR-M than in SWR-J mice. Serum obtained from SWR-M mice suppressed the extravasation of cells into the skin site of SWR-J mice challenged with oxazolone. The decrease in cellular influx in SWR-M mice occurred in areas of mite infestation (skin of neck and shoulder) as well as in areas not infested with mites (the ears). SWR-M mice also gave evidence of enhanced vascular permeability. A possible role for histamine in the inhibition of contact sensitivity in mite-infested mice is discussed.

Animals↗
PubMed1979

Suppression of IgE antibody formation in mice: requirement for T-T lymphocyte interaction.

Norman SWR mice injected with syngeneic spleen cells from ovalbumin (Ov)-primed mice were unable to make IgE anti-Ov antibodies when challenged with alum-pertussis-Ov. Immune T lymphocytes were shown to be responsible for the inhibitory effects of adoptively transferred spleen cells. Treatment of recipient mice with mild x-irradiation or with cyclophosphamide 2 or 3 days before cell transfer resulted in abrogation of the suppressor effect of immune cells. The injection of T lymphocytes into x-irradiated milce restored the suppressive effect of immune cells. It thus appears that immune T cells provide the stimulus for activation of suppressor T cells of the host. Although the generation of suppression is antigen-specific, the expression of suppression appears to be nonspecific.

Alum Compounds↗
PubMed1979

IgE antibody response to mite antigens in mite infested mice.

Mice infested at birth with the mouse mite Myocoptes musculinus developed positive skin tests to mite antigens at the age of 5 weeks. Serum IgE antibodies directed against mite antigens were first detected at 6 weeks of age and high levels of IgE were present as long as 1 year later. Similar kinetics of IgE formation were observed in mice infected as adults. Mast cell degranulation by mite extract was demonstrated in connective tissue obtained from the skin of mite infested mice.

Aging↗
PubMed1978

Suppressor T cells for IgE and IgG in Peyer's patches of mice made tolerant by the oral administration of ovalbumin.

A single oral dose of ovalbumin (Ov) resulted in inhibition of IgE formation in mice subsequently immunized i.p. with Al(OH)3-Ov. Repeated feeding of Ov (on alternate days for 2 weeks) induced the formation of detectable suppressor cells in Peyer's patches and spleen. Suppression was demonstrated by the ability of adoptively transferred Peyer's patch or splenic lymphocytes from Ov-fed tolerant mice to inhibit IgE formation in Ov-immunized syngeneic recipients. Suppressor cells could be induced by feeding mice as little as 100 microgram of Ov on alternate days for 2 weeks. Suppression was specific and Peyer's patch lymphocytes were shown to be more effective suppressors than splenic lymphocytes.

Administration, Oral↗
PubMed1977

Inhibition of IgE production of mice by non-specific suppressor T cells.

SWR adult normal splenic T lymphocytes injected into irradiated syngeneic mice along with immune spleen cells can suppress IgE formation by the immune cells. Spleen cells from 1- or 3-week-old mice, however, are not effective in abrogating IgE synthesis. The T lymphocytes which are induced to become suppressors for IgE are hydrocortisone-resistant and anti-thymocyte serum-sensitive. Normal SJL mice, a poor reagin-producing strain, do not appear to have more suppressor cells or cells which are more easily stimulated to become suppressor cells than a good reagin-producing strain (SWR).

Animals↗
PubMed1977

Enhanced reaginic antibody formation to ovalbumin in mice given repeated injections of concanavalin A.

Mice injected with 4 weekly doses of concanavalin A arabinogalactan complex (Con A-AG) give a secondary type of IgE response when they encounter ovalbumin (Ov) for the first time. The IgE-enhancing effect of Con A-AG immunization applies to Ov but not to various other antigens. However, no cross-reaction appears to exist at the humoral level between Con A and Ov. The apparent priming of mice to Ov by Con A can be adoptively transferred to syngeneic irradiated recipients by spleen cells.

Adjuvants, Immunologic↗
PubMed1976

The suppressive effect of the interferon on the ability of mouse spleen cells synthesizing IgE to sensitize rat skin for heterologous adoptive cutaneous anaphylaxis.

Mouse spleen cells which are synthesizing IgE can sensitize rat skin for HACA. When such cells are treated with mouse interferon, their ability to sensitize rat skin is reduced or completely inhibited. Two hundred PRD50 units of interferon reduced HACA reactions by more than 50%; 600 units gave complete inhibition. Interferon may act by inhibiting IgE synthesis and/or release.

Animals↗
PubMed1975

Enhancement of reaginic antibody formation in the mouse by concanavalin A.

SWR mice injected with Concanavalin A (Con A) and Ovalbumin (Ov) one immediately after the other or with mixtures of Con A and Ov produced reaginic antibodies to Ov (titer 1:80) after primary immunization and reaginic antibody levels of 1:640 after secondary immunization. Mixtures of Con A and Ov were shown to contain soluble complexes which could be precipitated by 20% polyethylene glycol (PEG). Mixtures of Con A and human serum albumin or Con A and bovine serum albumin did not form soluble complexes and did not induce detectable reagin formation. The possible role of soluble of Con A-Ov complexes in stimulating reaginic antibody is discussed.

Adjuvants, Immunologic↗