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Biomedical subjects

L S Sokurenko

Publications and source records attributed to L S Sokurenko.

3 recordsLinked to original sources
PubMed1989

[A method of determining allergic changes in leukocytes].

The new technique for the detection of hypersensitivity to allergens is based on the fact that after 2 hrs incubation of citrate blood with the allergen the leukocytes are destroyed and K ions leave the cell. Potassium level in the supernatant is measured by flame photometry. This method has been tried in examinations of 81 patients with pulmonary tuberculosis and aspergillosis and 18 healthy donors. The method has proved to be specific and informative for the detection of tuberculin and fungal allergy. It is less labor-consuming than the determination of the neutrophil injury index and more objective, since its results are recorded instrumentally.

Aspergillosis↗
PubMed1989

Macrophage-lymphocyte interaction in the formation of delayed-type hypersensitivity.

Macrophage-lymphocyte interaction was studied on 121 CBA mice during a 2-hour contact of lymph-node cells of non-immune mice with a monolayer of peritoneal macrophages of BCG-immunized mice and subsequent intravenous administration of 4.10(7) pre-incubated lymphocytes to syngenic recipients. Sensitivity to tuberculin was demonstrated in the recipients by means of blast-transformation reaction of spleen cells (stimulation index was evaluated according to incorporation of 3H-thymidine--SI = 1.32 +/- 0.40) using administration of tuberculin into the paws (Mantoux reaction--MR = 0.11 +/- 0.02 mm) and the cytotoxic effect (CTE) of the lymphocytes on tuberculin-loaded sheep-cell erythrocytes whose disintegration was assessed according to discharge of iron from the target cells (CTE = 13.98 +/- 2.73%). At transfer of intact lymphocytes after contact with non-immune macrophages, SI = 1.046 +/- 0.019; MR = 0.014 +/- 0.002 mm; CTE = 0.214 +/- 0.048%. The treatment of lymphocytes with indomethacin during the contact with macrophages induced idvere changes in the indices of delayed-type hypersensitivity (DTHS). The conclusion has been drawn that the antigen-presenting ability of macrophages can materialize in vitro.

Animals↗