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L S Strochkova

Publications and source records attributed to L S Strochkova.

12 recordsLinked to original sources
PubMed1990

[Hyposelenoses].

Literature on the various manifestations of the selenium deficiency in humans and animals is presented. Mechanisms of the biological effects of selenium brought about by glutathione peroxidase are considered. Biogeochemical anomalies, parenteral nutrition, malabsorption syndromes as conditions for the development of hyposelenosis are characterized as well as their manifestations (white-muscular disease, myopathies, cardiomyopathies, Keshan disease, Kashin-Beck disease). Selenium deficiency facilitates the development of the ischemic heart disease, myocardium infarction, certain types of malignancy and so on. The attention is drawn to the link of the selenium deficiency with imbalance of other trace elements.

Animals↗
PubMed1979

[Effect of the inhibition of a synthesis processes in the S-period on the subsequent course of mitosis in a synchronized Chinese hamster cell culture].

The transcription of all nuclear RNA and rRNA was inhibited in the synchronized Chinese hamster cell culture by actinomycin D and protein synthesis was suppressed by pyromycin during the first and the second parts of the S period of the mitotic cycle. A decrease of the mitotic cell activity was revealed during the first and second waves of cell proliferation after the synchronization irrespective of the time of the antibiotic administration. At the same time there was noticed an increase of such pathological mitoses as C-mitoses and chromosome scattering in the metaphase, whereas suppression of rRNA synthesis determined the delay of cell going out of mitosis (an increase of the anaphase and telophase cell portion) mostly correlated with such type of pathological mitoses as chromatid and chromosome bridges. The protein synthesis depression during the first half of the S-period failed to influence the mitotic regimen of the cell culture during the first, nearest division, but suppressed considerably the cell proliferation during the distant proliferation wave. It is suggested that the cells entered the S-period having in store the proteins for initiation and supporting the genom reduplication at least during the first stage of this process.

Animals↗
PubMed1979

[Pathology of mitosis after cellular recovery from a metaphase block].

The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.

Animals↗
PubMed1979

[DNA content and the endometrial cellular mitotic activity in the phase of menstrual bleeding].

Endometrium obtained during menses from 46 healthy women in reproductive age was investigated morphologically and cytospectrophotometrically in order to solve the problem on the source of the cells reepithelizing the uterine mucous membrane after desquamation. It was stated that desquamation takes place not in the whole functional layer of endometrium, some mucous fragments, covered with persisting luminal epithelium, are always preserved. During endometrial regeneration the cells of the luminal and glandular epithelia and those of endometrial stroma are predominantly diploid. The amount of premitotic cells in population is so small that they cannot secure any intensive cellular proliferation. Mitogenesis in endometrium is stimulated only after a complete restoration of the epithelial layer. It is suggested that persisting luminal epithelium is the source of cells for reepithelization; they migrate towards endometrial "wounds" and repair defects in the uterine mucosa during the regeneration phase.

Adult↗
PubMed1978

[Effect of inhibitors of RNA and protein synthesis on the course of mitosis in a synchronized culture of Chinese hamster cells].

The synthesis period of various types of RNA and proteins during the interphase related to the immediate after synchronization mitosis was investigated on teh synchronized culture of Chinese hamster cells with inhibitors. Analysis of MI and forms of mitosis pathology indicated the proteins functionally related to the cell division to be mainly synthesized during the second half of the interphase. The most important action of pyromycin during this time is the induction of C-mitosis on account of suppression of proteins synthesis responsible for the spiralization of chromosomes during the prophase. Inhibition of the r RNA transcription at any stage influence the cytotomy and reconstruction of daughter nuclei, this being accompanied by the delay of the cell exit from the mitosis. In difference from pyromycin, a high dose of AMD as an inhibitor of the whole cell RNA synthesis was most effective during the first half of the interphase. It is suggested that during this period at least three kinds of RNA are synthesized which are functionally related to the immediate mitosis in a different way: RNA for genome reduplication, RNA for the tubulin synthesis, and RNA which is the division spindle component.

Animals↗
PubMed1978

[Remote changes in the course of mitosis in synchronized cultures of Chinese hamster cells following inhibition of RNA and protein synthesis].

The effect of inhibition of the synthesis of some types of RNA and proteins was determined in the synchronized culture of Chinese hamster cells at various stages of the interphase on the course of remote mitosis. Analysis of MI and some forms of pathological cell division showed that the action of different doses of AMD and pyromycin during the first part of the interphase provoked an identical effect--C-mitosis at the immediate and remote waves of cell division. Suppression of the synthesis of whole cell RNA and proteins during the second half of the interphase was accompanied by a metaphase cell delay with scattering chromosomes, this indicating derangement of the synthesis of the division spindle component. It is suggested that proteins (tubulins) and RNA participate in the organization of the division spindle during remote mitosis as a "reserve pool".

Animals↗
PubMed1978

[Characteristics of mitosis pathology in a Chinese hamster cell culture with disorders of the protein-synthesizing system].

Disturbance of protein synthesis with puromycin and transcription of chromosomal and ribosomal RNA with actinomycin D was followed by marked changes in the normal course of mitosis. There was noted an increase in number of colchicin-like mitoses (C-mitoses), and particularly, sometimes, fragmentation of their cytoplasm with the formation of cluster-like structures. It is suggested that development of C-mitosis was connected not only with destruction in the mitotic apparatus formation system, but also with the block of synthesis of one of the chromosomal proteins, stabilizing DNA strands spiralization. Another form of pathological mitosis occurring under conditions of suppression of the metabolic processes ("hollow metaphase plate") was linked not only with the chromosomal alterations. Selective suppression of the ribosomal RNA transcription led to an evident anaphase delay and to coupling of telomere regions of chromosomes. This phenomenon is apparently associated with destruction of the "protective cover" of chromosomes, formed by RNA of the disintegrating nucleoli and RNA of perichromatin granules.

Animals↗
PubMed1978

[DNA concentration in cells of the glandular epithelium of the endometrium during the menstrual bleeding phase].

DNA content in the endometrial gland cells (9 women of fertile age) was studied during menstruation by microspectrophotometry. It was shown that DNA content (mainly 2n) remained unchanged during different days of menstruation, both during the desquamation (the 1st--2nd days of the cycle), and during the regeneration stage (the 3rd--4th days). Along with this the mitotic activity in the glandular epithelium was absent completely (36 samples of endometrium).

Cell Biology↗
PubMedDate not supplied

[Ultracytochemical study of adenylate cyclase activity in Hela cell culture exposed to fluorine].

Electron microscopy has been used to study the adenylate cyclase activity in Hela cells after the incubation with 1.5 p. p. m. of fluoride for 5.30 and 60 minutes. It is established that the action of fluoride is accompanied by the increased enzyme activity in cell plasmalemma. Besides, the adenylate cyclase activity is revealed in the membrane of lipid droplets. In the mitotic cells a decrease of the enzyme activity in plasma membrane is observed parallel with its simultaneous increase in the metaphase chromosomes.

Adenylyl Cyclases↗
PubMedDate not supplied

[Ultrastructural examinations of HeLa cells exposed to cadmium ions].

Destructive changes in cadmium-treated HeLa cells affecting practically all the cell structures and organelles, were observed. Side by side with it compensation and adaptation responses of cells, which in definite period reduced cell pathological effect of this microelement were revealed.

Cadmium↗