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Biomedical subjects

L Samochowiec

Publications and source records attributed to L Samochowiec.

At least 19 recordsLinked to original sources
PubMed1992

Essential phospholipids modify immunological functions and reduce experimental atherosclerosis in rabbits.

Atherosclerosis was induced in male mongrel rabbits with a high-fat diet and the influence of essential phospholipids (EPL) on plaque formation, parameters of lipid metabolism and immunological functions was studied. When EPL were added to the high-fat diet there was a significant reduction in the area of atherosclerotic involvement of the aorta. The serum concentration of lipids decreased, often to normal values, and cholesterol esterified with polyunsaturated fatty acids appeared. Normalization of the malonyldialdehyde level in plasma was accompanied by a decrease in the concentration of ascorbate free radicals in blood and liver. The high-fat diet caused a depression of both non-specific and specific immune functions studied. With EPL in the diet the tests showed near normal or normal values. It is inferred from these results that a normal state of the immune system is important for preventing the progress of atherosclerotic changes. This is discussed with reference to the role of some immune cells in the metabolism of lipids and to participation of essential phospholipids in plasma membrane functions.

Animals↗
PubMed1992

Caffeine reduces the hepatotoxicity of paracetamol in mice.

Paracetamol causes extensive liver damage when taken in overdose quantities; however, it is less hepatotoxic when administered in combination with caffeine. The present work in mice was undertaken to study the effect of caffeine on mortality rates and biochemical and histological parameters of liver damage after administration of toxic doses of paracetamol. It was found that caffeine markedly increased the survival rate after administration of a dose of paracetamol that was lethal to 50% and 100% of mice, reduced liver damage as assessed by serum glutamic pyruvic and glutamic oxaloacetic transaminase activities, partially prevented the depletion of reduced glutathione and reduced histological changes to the liver accompanying paracetamol intoxication. The results support the possibility that caffeine might be useful for the treatment of paracetamol intoxication in humans.

Acetaminophen↗
PubMed1992

Padma 28 modifies immunological functions in experimental atherosclerosis in rabbits.

The effect of Padma 28 on selected parameters of humoral and cellular immune reactions in rabbits subjected to experimental atherosclerosis was studied. The drug significantly reduced the size of atherosclerotic plaques in the aorta and restored to a varying extent the immune functions studied. The possible mechanism by which Padma 28 may exert its anti-atherosclerotic action is discussed in the scope of the immunological theory of atherosclerosis.

Animals↗
PubMed1991

Effect of selenium and vitamin E on the development of experimental atherosclerosis in rabbits.

Male mongrel rabbits, divided into 5 groups (1) controls, (2) animals receiving a high-fat diet (HFD) containing cholesterol and coconut oil, (3) HFD + selenium, (4) HFD + vitamin E, (5) HFD + selenium + vitamin E, were treated for 12 weeks. In the groups receiving selenium and/or vitamin E, the elevation of serum total lipids, beta-lipoproteins, total cholesterol and triglyceride was markedly suppressed. HDL cholesterol in these groups of animals was increased. The cytochrome P-450 content in liver microsomes was increased, and the concentration of malondialdehyde in the blood plasma of rabbits was significantly decreased, while thyroid hormones (T4, T3), cortisol and insulin level were increased. Surface area of the lipid deposits at 12 weeks measured planimetrically averaged 76% in HFD-fed animals but only 28% in selenium + vitamin E treated rabbits. The important finding of this study is that combination of selenium and vitamin E, results in an intensified effect on the improvement of metabolic processes and on the reduction of atherosclerotic plaque formation.

Animals↗
PubMed1991

Hepatoprotective effect of selenium and vitamin E in rabbits fed a high-fat diet.

The high-fat diet consisted of cholesterol, hydrogenated coconut oil, and cholic acid. In the blood serum and in liver homogenate lipid content, cholesterol, and triglycerides were assayed. Cytochrome P-450 concentration in liver microsomes was also estimated. In animals receiving selenium and vitamin E, the content of lipid fractions in the blood serum and liver homogenate fell, while the cytochrome P-450 content in the liver microsomes was markedly elevated. The intensified protective effect of vitamin E and selenium applied in combination against changes induced in the liver of animals receiving a HFD was confirmed by macroscopic and microscopic examination of the organ.

Alanine Transaminase↗
PubMed1991

Protective effect of selenium and vitamin E against changes induced in heart vessels of rabbits fed chronically on a high-fat diet.

The effect of selenium and vitamin E on lipid level in the blood serum, on the oxygen free radicals generation and on morphology of heart was tested after separate and combined administration to mongrel male rabbits fed on a high-fat diet (HFD). The lipid level and oxygen free radical generation was depressed markedly in animals fed on a HFD and receiving simultaneously selenium and vitamin E. In animals on a HFD the walls of the heart vessels were thickened, always to their complete obliteration. The presence of lipid droplets in endocardium could be observed as well. The hearts of the rabbits receiving selenium showed markedly fewer atheromatously changed vessels. Moreover, no accumulation of lipid droplets was seen in the endocardium of these animals. The least atherosclerotic alterations were observed in the myocardium of rabbits given HFD with addition of selenium and vitamin E in combination, no accumulation of lipids was shown in endocardium of these rabbits. An important finding of this study is, that the combination of selenium and vitamin E results in an intensified protective effect against changes evoked in the heart muscle of rabbits fed on a HFD.

Animals↗
PubMed1989

[The effect of pollen on the changes in the liver of laboratory rats evoked by ethionine, carbon tetrachloride, allyl alcohol and galactosamine].

Doses of 50 mg/kg body weight and 200 mg/kg of Cernitin T 60 and Cernitin GBX may be used over 14 days for effective protection of rat liver cells from toxic action of ethionine. Application of CCl4 caused damage to the liver of rats. Such damage may be mitigated by both Cernitin preparations, particularly by Cernitin T 60. The damage was further reduced by Cernitin, following administration of allyl alcohol, with increase in transaminase, phosphatase, and bilirubin activities being used as criteria for measurement. The liver-protecting effect of Cernitin was confirmed in histopathological investigations. Cernitins prevented much of the damage actually caused by galactosamine.

1-Propanol↗
PubMed1989

[Effect of essential phospholipids (EPL) on the course of chronic poisoning with organic solvents in rats].

Male Wistar rats were exposed in toxicological camera to the mixture of organic solvents. Composition and concentrations of the mixture were similar to the conditions in the production hall. The influence of essential phospholipids (EPL) on changes in the liver caused by the organic solvents were studied. Animals were exposed for 6 months. Histochemical and histological evaluation was performed after 2 and 6 months. In the liver of intoxicated animals marked vacuolar degeneration and diminution of glycogen contents were observed. Simultaneously, the protective effect of EPL was noted. It was expressed by distinctly decreased vacuolar degeneration, probably corresponding to neutral fat in hepatocytes as well as by normalization of glycogen contents.

Animals↗
PubMed1987

Study on the antioxidant properties of pollen extracts.

The study on the antioxidant and hypolipidemic effect of pollen extracts (Cernitins) was conducted in male mongrel rabbits and Wistar rats. The animals were fed a high-fat diet (HFD) composed of cholesterol, coconut oil and cholic acid, and received pollen extracts (Cernitins) orally (the rabbits over a period of 12 weeks and the rats over a period of 2 weeks). The levels of malondialdehyde (MDA) as an indication of the degree of peroxidation and lipids (cholesterol, triglyceride, separation of lipoproteins into fractions) were measured. The study demonstrated the reduction of MDA concentrations under the influence of Cernitins, suggesting their antioxidant properties. Total cholesterol and triglyceride content was also decreased.

Animals↗
PubMed1986

Effect of pollen extract on the development of experimental atherosclerosis in rabbits.

Our previous studies have shown that the pollen extract, Cernitin, reveals lipid-lowering properties in animals and humans. The present study was designed to investigate the influence of Cernitin on the development of experimental atherosclerosis in rabbits over a period of 12 weeks. Forty male mongrel rabbits were divided into 4 equal groups: (1) controls, (2) animals receiving high-fat diet (HFD) containing cholesterol and coconut oil, (3) HFD + pollen extract, and (4) HFD + clofibrate. The most pronounced reduction in lipid metabolism and in the severity of plaque formation occurred after the pollen extract had been applied. The total cholesterol content in serum and liver homogenate was depressed by 67% and 45%, respectively, while the serum HDL cholesterol and alpha-lipoproteins level was increased by 19% and from 7.73% to 21.73% respectively. The cytochrome P-450 content in the liver microsomes was elevated by 98% (nmol/g liver). Atherosclerotic plaque intensity at 12 weeks, measured planimetrically, averaged 85.5% in HFD-fed animals vs 33.7% in pollen extract-treated rabbits. These findings suggest that Cernitin, in addition to significantly lowering serum lipid levels in rabbits on an experimental diet, may modify lipid deposition in major arteries.

Animals↗
PubMed1985

The protective effect of pollen extracts against allyl alcohol damage of the liver.

In male Wistar rats the hepatoprotective effect of pollen extracts (Cernitins) agains. orally introduced 1% allyl alcohol (0.4 ml per 100 g body weight) was investigated Cernitins were applied orally at 0.6, 24 and 30 h after allyl alcohol administration. After 48 h an autopsy was performed and blood was collected for biochemical tests. Liver damage was evaluated by measurement of aminotransferases (AspAT, AlAT) and alkaline phosphatase activity, total bilirubin level in the blood serum as well as by histological examination of the livers. Cernitins significantly reduced the serum enzymes elevations induced by allyl alcohol. The hepatoprotective properties of Cernitins were confirmed by histopathological studies.

1-Propanol↗
PubMed1985

The effect of Cernitins on galactosamine-induced hepatic injury in rat.

Cernitins correspond to microbiologically fermented pollen extract. Cernitin T60 contains mainly water soluble, while Cernitin GBX mainly fat soluble substances. The aim of the present work was to examine the effect of Cernitins on the d-galactosamine-induced liver damage in rat. It has been shown, that galactosamine administration to rat resembles viral hepatitis both biochemically and histologically. Our studies proved, that Cernitin T60 given orally or intraperitoneally inhibited or counteracted the elevation of amino transferases activity and the inflammatory process, necrosis and steatosis of the liver cells. The protective effect of Cernitin GBX on the liver parenchyma was only slightly expressed. It is concluded, that application of pollen extracts in patients with liver diseases should be considered.

Animals↗
PubMed1980

An experimental model of hyperlipemia.

Rats were given two kinds of diet: fat-rich (coconut oil, cholesterol, cholic acid) and fat-carbohydrate diet (coconut oil, margarine, cholesterol, wheat flour) for a period of 18 weeks. In the blood serum the triglyceride-cholesterol level and the electrophoretic pattern of lipoproteins were determined. Experimental models of experimental hyperlipemia adequate to type IIb and IV according to Fredrickson were obtained.

Animals↗