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Biomedical subjects

L Sarkadi

Publications and source records attributed to L Sarkadi.

14 recordsLinked to original sources
PubMed1997

The functional and morphological damage of ischemic reperfused skeletal muscle.

Skeletal muscle is frequently damaged by ischemia-reperfusion both caused by direct injury and also by surgery. The purpose of the present experiments was to examine how the different types of skeletal muscles (fast and slow) react functionally and morphologically after 1 and 2 h of ischemia followed by different periods of reperfusion. The fast-twitch (musculus extensor digitorum longus, EDL) and the slow-twitch (musculus soleus, SOL) muscle of Wistar rats were prepared. They were stimulated in vivo, either directly or indirectly at different reperfusion times following tourniquet ischemia, and the contraction force of the muscles was recorded. The morphological changes were examined by light microscopy. At early reperfusion times, the contraction force of the EDL muscle was reduced by 40 and 90% after 1 and 2 h of ischemia, respectively. The contraction force was about 50% at the end of a 2-week reperfusion period in the 1-hour ischemia group and it increased significantly (from 5 to 38%) during the second week if the ischemia lasted for 2 h. Reduction of contraction force in the SOL muscle was over 50 and 90% following 1 and 2 h of ischemia, respectively, and it started to improve from the 2nd week. Morphological changes of the two types of muscle were identical. At early reperfusion times granulocytes were seen in the blood vessels adhering to the endothelium. 24 h later neutrophil granulocytes migrated into the endomysium and thereafter into the perimysium. One week after 1 h of ischemia both muscles showed normal histology. However, the structural regeneration process only started at the end of the 1st week of reperfusion after 2 h of the ischemic damage. The following conclusions can be drawn. (1) There is functional morphological evidence of ischemic and reperfusion injury in both muscles after 24 h and also after 1 week of reperfusion. (2) Functionally, the two types of muscles regenerate differently, i.e. the SOL starts to regenerate earlier than the EDL. (3) Morphologically the two types of muscle show the same reactions. An increase in the time of ischemia from 1 to 2 h delays the regeneration processes.

Animals↗
PubMed1996

The functional damages of ischemic/reperfused skeletal muscle.

Skeletal muscle is frequently damaged by ischemia-reperfusion when exposed to direct injury or in the surgical practice. The purpose of the present experiments was to examine how the different types of skeletal muscles (fast & slow) react functionally to one and two hours of ischemia followed by two weeks of reperfusion. The fast-twitch (m. extensor digitorum longus/EDL) and the slow-twitch (m. soleus/SOL) muscle were prepared. They were stimulated, in vivo, either directly or indirectly at different reperfusion times following tourniquet ischemia, and the contraction force (CF) was recorded. CF of the EDL was reduced over 40% and 90% of the control value during the first 24 hours of reperfusion after 1 and 2 hours of ischemia, respectively. It was about 50% at the end of the 2nd week in the one-hour group. CF increased significantly during the second week if ischemia lasted for two hours. Reduction of CF in the SOL muscle was over 50% and 90% following one and two hours of ischemia, respectively. It further decreased in the 1-hour group, and it started to regenerate from the second week after 2 hours of ischemia. It is concluded that 1. two hours of ischemia causes significantly more severe damages in both types of skeletal muscles than one hour. 2. There is a reperfusion injury in both muscles during the first week of reperfusion. 3. The two types of muscles regenerate differently, i.e. the SOL starts to regenerate earlier than the EDL.

Animals↗
PubMed1994

Biochemical studies of some non-conventional sources of proteins. Part 7. Effect of detoxification treatments on the nutritional quality of apricot kernels.

Detoxification of apricot kernels by soaking in distilled water and ammonium hydroxide for 30 h at 47 degrees C decreased the total protein, non-protein nitrogen, total ash, glucose, sucrose, minerals, non-essential amino acids, polar amino acids, acidic amino acids, aromatic amino acids, antinutritional factors, hydrocyanic acid, tannins and phytic acid. On the other hand, removal of toxic and bitter compounds from apricot kernels increased the relative content of crude fibre, starch, total essential amino acids. Higher in-vitro protein digestibility and biological value was also observed. Generally, the detoxified apricot kernels were nutritionally well balanced. Utilization and incorporation of detoxified apricot kernel flours in food products is completely safe from the toxicity point of view.

Amino Acids↗
PubMed1990

[Preoperative diagnosis of breast tumors in our clinic].

Between 1983 and 1988 197 patients underwent mammography and fine needle aspiration cytology procedure beside physical examination before operation for breast tumor in our clinic. The sensitivity of mammography and fine needle aspiration cytology were 81.9 and 66.1%, their specificities were 91.2 and 95%, the specificity of physical examination was 97.7%. When all three examinations indicated malignancy, this was confirmed by histology without exception. When the examinations showed benign lesion in concert, four of the 26 diagnoses were false, histology proved breast cancer smaller than 2 centimetres in diameter without lymph node metastases in each case. Those aspirations which were considered unsatisfactory because of the lack of cells supported the diagnosis of a benign tumor raised by physical examination and mammography. Our data indicate that the diagnosis yielded by the combination of the three examinations may support therapy more than it does in the present.

Breast Neoplasms↗