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Biomedical subjects

L Schmidt

Publications and source records attributed to L Schmidt.

At least 73 records · Page 4Linked to original sources

Comparison of self-reported and medical record health care utilization measures.

Self-reported utilization of health care services is important in epidemiological studies and in health care planning, policy, and research, and the accuracy of such information is essential. This study assessed the validity of self-reported utilization of health care services in a randomly selected cohort of 500 community-dwelling men aged 40 to 79 years in Olmsted County, Minnesota. Men had previously completed a self-administered questionnaire that elicited information on utilization of health care services; questions included total inpatient hospital nights in the previous year, total ambulatory physician visits in the previous year, and physician visits in the previous 2 weeks. The complete community medical records of the men were reviewed, and the accuracy of self-reported information was evaluated by assessing the difference between self-reported utilization and actual medical record utilization as ascertained from the medical records, and the distribution of these differences. Exact agreement was 93% for inpatient hospital nights, 91% for ambulatory physician visits in 2 weeks, and 30% for ambulatory physician visits in the previous year. There was an increase in the variability of the difference between self-reported and medical record utilization with an increasing number of inpatient nights or ambulatory physician visits, and a significant bias toward underreporting with an increasing number of ambulatory physician visits in the previous year. These findings suggest that self-reported inpatient nights in the previous year and ambulatory physician visits in 2 weeks are reasonably accurate, but self-reported ambulatory physician visits in the previous year may be less accurate and likely to be biased toward underreporting at higher numbers of visits. This information should be taken into account by researchers and health planners using self-reported measures of utilization.

Adult↗

WHO cross-cultural applicability research on diagnosis and assessment of substance use disorders: an overview of methods and selected results.

The cross-cultural applicability of criteria for the diagnosis of substance use disorders and of instruments used for their assessment were studied in nine cultures. The qualitative and quantitative methods used in the study are described. Equivalents for English terms and concepts were found for all instrument items, diagnostic criteria, diagnoses and concepts, although often there was no single term equivalent to the English in the languages studied. Items assuming self-consciousness about feelings, and imputing causal relations, posed difficulties in several cultures. Single equivalent terms were lacking for some diagnostic criteria, and criteria were sometimes not readily differentiated from one another. Several criteria--narrowing of the drinking repertoire, time spent obtaining and using the drug, and tolerance for the drug--were less easy to use in cultures other than the United States. Thresholds for diagnosis used by clinicians often differed. In most cultures, clinicians were more likely to make a diagnosis of drug dependence than of alcohol dependence although behavioural signs were equivalent. The attitudes of societies to alcohol and drug use affects the use of criteria and the making of diagnoses.

Alcoholism↗

Patterns in the institutional encounters of problem drinkers in a community human services network.

It is useful to view the social handling of alcohol problems in US communities from the perspective of a whole network of human service systems that share in the burden of identifying and responding to problem drinkers. This analysis examines the management of alcohol problems in different community service systems by mapping patterns in the institutional encounters of problem drinkers across alcohol treatment, drug treatment, mental health treatment, social welfare and criminal justice systems in a single US community. Findings highlight the prominence of large bureaucratic systems for social welfare and criminal justice as sources of referrals for smaller service systems offering treatment for alcohol problems. However, large proportions of problem-drinking service recipients in the community remain exclusive clients of the welfare and criminal justice systems, making no contact with therapeutically orientated service settings. Compared with problem drinkers who obtain treatment services, problem drinkers on the case-loads of criminal justice and welfare agencies tend to be younger, of higher socio-economic status, are more likely to be male, and tend to drink less heavily and to experience fewer symptoms of alcohol dependence. Given the distinctive characteristics of problem drinkers found exclusively in criminal justice and welfare settings, it may be advisable for communities to introduce early intervention programs in these systems that target services to this particular subgroup of problem drinkers.

Adolescent↗

A seasonal staffing model.

The staffing of a nursing unit provides a unique challenge when that unit experiences significant seasonal fluctuations in census. Although overtime, perdiem, or agency personnel are staffing alternatives to meet seasonal fluctuations in census, they are costly alternatives that are not the most efficient use of human resources. The authors present a seasonal staffing model that reallocates employee work hours in a pattern that closely matches the seasonal work load trends of the department.

Cost Savings↗

Intestinal distribution of human Na+/H+ exchanger isoforms NHE-1, NHE-2, and NHE-3 mRNA.

The identity of Na+/H+ exchanger (NHE) isoforms in the human small intestine and colon and their role in vectorial Na+ absorption are not known. The present studies were undertaken to examine the regional and vertical axis distribution of NHE-1, NHE-2, and NHE-3 mRNA in the human intestine. Ribonuclease protection assays were used to quantitate the levels of mRNA of these isoforms in various regions of the human intestine. In situ hybridization technique was used to localize NHE-2 and NHE-3 mRNA in the colon. The NHE-1 isoform message was present uniformly throughout the length of the human intestine. In contrast, mRNA levels for human NHE-2 and NHE-3 isoforms demonstrated significant regional differences. The NHE-3 abundance was found in decreasing order: ileum > jejunum > proximal colon = distal colon. The NHE-2 message level in the distal colon was significantly higher than in the proximal colon but was evenly distributed in the small intestine. In addition, NHE-2 mRNA was present in surface epithelial cells as well as in cells of the crypt region, suggesting the presence of NHE-2 message throughout the vertical axis of the colonic crypts. In contrast, NHE-3 mRNA was localized to surface colonocytes in the proximal colon. On the basis of this tissue-specific localization of NHE-2 and NHE-3 mRNA, it can be speculated that the relative contribution of NHE-2 and NHE-3 isoforms in Na+ absorption in the human intestine may be region specific, and these putative apical isoforms may be differentially regulated.

Amino Acid Sequence↗

Renal cell carcinoma of end-stage renal disease: a histopathologic and molecular genetic study.

Renal cell carcinomas (RCC) are responsible for the deaths of 3% to 4% of patients with ESRD. The clear cell carcinoma of the kidney, which comprises 80% of sporadic RCC within the general population, shows a deletion of gene sequences in the short arm of chromosome 3 (3p) in as many as 100% of cases. The von Hippel-Lindau tumor suppressor gene at 3p25-26 is found to be mutated in the nondeleted allele in 57% of these sporadic clear cell carcinomas. This study was undertaken to determine the histopathologic types of RCC occurring in ESRD patients in the United States and to investigate the frequency with which 3p genetic changes can be found in these ESRD tumors. Seventeen end-stage kidneys containing RCC were collected from 15 ESRD patients at ten US medical centers. The tumors were classified by Thoenes' histopathologic typing. DNA extracted from paraffin blocks of tumor and nontumorous tissue was analyzed by single-stranded conformational polymorphism analysis for von Hippel-Lindau mutations and by microsatellite amplification for deletion of 3p gene sequences. Twenty-one RCC were identified in the 18 kidneys. The 21 RCC were classified histopathologically as follows: clear cell, compact, three cases; chromophilic, tubulopapillary, 15 cases; chromophilic, compact, three cases. Among the three clear cell carcinomas, one showed 3p genetic loss. None of the chromophilic RCC showed a 3p deletion and none of 19 tumors studied by single-stranded conformational polymorphism analysis disclosed von Hippel-Lindau mutations. In contrast to the general population, clear cell RCC with 3p abnormalities represent only a small proportion of the renal carcinomas in this collection of ESRD tumors. The findings indicate that the genetic changes underlying the development of most ESRD tumors are different from those occurring in sporadic clear cell RCC and do not characteristically involve the inactivation of a 3p tumor suppressor gene.

Adult↗

Suppression of growth of renal carcinoma cells by the von Hippel-Lindau tumor suppressor gene.

Clear cell renal carcinomas are most frequently characterized by loss of function of both copies of the von Hippel-Lindau (VHL) disease gene, suggesting that the VHL gene product plays an important role in regulating renal cell proliferation. To directly assess the function of the VHL gene product, we transfected the wild-type VHL gene into two renal carcinoma cell lines that lacked normal expression of the gene. Expression of the wild-type VHL gene led to a dramatic suppression of growth in two renal carcinoma cell lines, A498 and UMRC6 in vitro, as measured by colony formation and direct cell counting. Transfection of a naturally occurring mutant VHL gene (nucleotide 713 G to A, Arg to Gln) did not lead to growth suppression of these renal carcinoma cells, nor did transfection of the wild-type VHL gene into two non-renal tumor cell lines that expressed the endogenous wild-type VHL gene. Expression constructs, which included the first ATG at nucleotide 214, were sufficient to produce the strongest growth suppression. These experiments provide direct evidence that the VHL gene product functions to suppress the growth of renal carcinoma cells and also provide a model for mapping the domains of the VHL protein important in suppressing tumor growth.

Base Sequence↗

Physician referrals for smoking cessation: outcome in those who show and don't show.

BACKGROUND: Only recently have clinical programs for smoking cessation become available in some medical centers. Counselor intervention services are provided to Mayo patients, most of whom are physician referred. Each patient receives a 45- to 60-min consultation and then is enrolled in a structured follow-up/relapse prevention program. METHODS: In 1989, 224 of 1764 patients referred by their physicians for consultation did not receive the consultation. This cohort was surveyed 1 year after referral to determine smoking status. We compared these patients to a cohort of 578 patients who were seen in 1988 for counseling and received the follow-up program. RESULTS: Of those referred, 87.3% received consultation. There was a 0.7%, 1-year point prevalence self-reported smoking cessation rate for those who were referred but did not follow through compared with 23.0% for those seen by the counselor and who received the follow-up program. CONCLUSIONS: In a medical setting, physician referral for nicotine dependence intervention and counselor-provided services is associated with a high attendance rate. Receiving counselor-provided consultation and follow-up was associated with a doubling of the stop rate. Such counselor services have substantial utility. There is a need for broader implementation of this type of service in medical centers.

Adult↗

The emergence of problem-drinking women as a special population in need of treatment.

This chapter chronicles the development of advocacy for improvements in alcohol treatment services for women during the 1970s and 1980s, tracing its influence in terms of real change in treatment systems in the United States. We follow the development of a "women's alcoholism movement" from its inception in the late 1970s through its transition during the late 1980s into a broader movement focused on drug abuse and perinatal addiction. We describe the new governing images of problem-drinking women that advocates presented, their claims about the nature of substance abuse problems in women, and their recommendations for a more "gender-sensitive" treatment system. We also review increased federal involvement in this issue over the course of the 1980s, as pressure mounted on policymakers to respond to the crisis over drug-exposed infants by making treatment services more accessible to women. The chapter concludes by considering these developments from the perspective of national treatment system statistics, finding modest growth in specialized and women-only treatment units, as well as moderate increases in the representation of women in substance abuse treatment caseloads.

Alcoholism↗

NHE-1 isoform of the Na+/H+ antiport is expressed in the rat and rabbit esophagus.

BACKGROUND & AIMS: Rabbit esophageal cells show an amiloride-sensitive Na+/H+ antiporter activity. Several distinct molecular isoforms of the Na+/H+ exchanger family (NHE) are reported to be present in the gastrointestinal tract of rats and rabbits. The aim of this study was to examine which isoforms are present in rabbit and rat esophageal cells and whether this isoform could be up-regulated by serum factors. METHODS: Specific primers designed from the rat NHE-1-4 and the rabbit NHE-1-3 isoform sequences were used for reverse-transcription polymerase chain reaction analysis with RNA from rabbit and rat esophageal cells. Ribonuclease protection assay was used to determine the serum-induced up-regulation of NHE-1. Antibodies raised against the NHE-1 C-terminal fragment were used for Western blotting with rabbit esophageal membranes. RESULTS: In both the rat and rabbit esophagus, only the NHE-1 isoform messenger RNA could be detected. The NHE-1 messenger RNA, detected in rabbit esophageal cells grown from tissue explants, was up-regulated by serum factors. The antibody detected a 95-kilodalton protein in esophageal cell membranes. CONCLUSIONS: The rabbit and rat esophagus exclusively express the NHE-1 isoform, hypothesized to be involved in cytoplasmic pH regulation. Therefore, the results of this study suggest a role for NHE-1 in protecting cells against gastric acid that is refluxed into the esophagus.

Animals↗

Somatic von Hippel-Lindau mutation in clear cell papillary cystadenoma of the epididymis.

Papillary cystadenoma of the epididymis is an uncommon benign lesion that may occur sporadically or as a manifestation of von Hippel-Lindau (VHL) disease. Neither immunohistochemical studies nor molecular genetic analyses of the VHL gene have been reported previously for this lesion. The authors describe two cases of clear cell papillary cystadenoma of the epididymis, both of which were initially confused with metastatic renal cell carcinoma. Both lesions showed positive immunohistochemical staining for low and intermediate molecular weight keratins (Cam 5.2 and AE1/AE3), EMA, vimentin, alpha 1-antitrypsin, and alpha 1-antichymotrypsin. Each was negative for CEA. Because clear cell papillary cystadenoma is similar to renal cell carcinoma histologically, and because both occur as components of the von Hippel-Lindau disease complex, the authors analyzed both cases for the presence of mutations in the VHL gene. A somatic VHL gene mutation was detected in one of the two tumors by polymerase chain reaction followed by single-strand conformation polymorphism analysis. Direct sequencing revealed a cytosine to thymine transition at nucleotide 694, resulting in the replacement of an arginine with a stop codon after the sixth amino acid of exon 3. As the VHL gene is believed to function as a tumor suppressor gene, VHL gene mutations may play a role in the initiation of tumorigenesis in sporadic cystadenomas of the epididymis.

Adenocarcinoma, Clear Cell↗

Limited sampling models for reliable estimation of etoposide area under the curve.

Limited sampling models are able to estimate the area under the concentration-time curve (AUC) from plasma concentrations measured at only a few time points. The purpose of this study was to establish a model estimating etoposide AUC independently of specific chemotherapy protocols, underlying malignancies, concomitant diseases and age. Pharmacokinetic parameters were measured in 30 patients treated with polychemotherapy including etoposide (80-150 mg/m2). Etoposide analysis was performed by thin layer chromatography and consecutive quantitative sample detection by 252Cf-plasma desorption mass spectrometry. Data from the first 15 patients formed the training set. Based on the training data, five different models were generated, with the multiple regression coefficient r ranging from 0.91 to 0.96. The following model was selected as "most accurate": AUC = 343 (min)C4h(micrograms/ml) + 650(min)C8h(micrograms/ml) + 1252 (min micrograms/mol), where C4h is the plasma concentration of etoposide at 4 h after the end of infusion and C8h at 8 h. This model was validated on the test set, comprising the data of the remaining 15 patients. The mean predictive error (MPE) was -0.2% and the root mean square predictive error (RMSE) was 4.7%. When used for a large number of patients, this practicable and simple model is an instrument for use in prospective studies, to measure a correlation between drug dosage and efficacy or toxicity of the drug.

Adult↗

The Community Epidemiology Laboratory: studying alcohol problems in community and agency-based populations.

This paper describes the Community Epidemiology Laboratory (CEL) of the Alcohol Research Group, a project focusing on the epidemiology of alcohol problems and community responses to those problems. Since 1980, a series of probability surveys have been conducted in the general population and in a wide range of health and social service agencies in a northern California county. The agency-based samples have included all agencies or representative samples of each system (alcohol treatment, drug treatment, mental health treatment, emergency rooms, primary health care, criminal justice and welfare) with probability samples of clients interviewed at intake. These studies have addressed questions regarding (1) the burden and epidemiologic description of alcohol problems in a community; (2) the relationship of alcohol to other health and social problems; (3) the influence of social policy on the constitution of services and care of individuals; and (4) health services issues of access and pathways to treatment. The project is designed for comparable analysis of studies with special focus on women and ethnic minorities. This paper provides an overview of the CEL, including its major research questions, overall design, sampling principles, methodological issues and measures. It highlights the main findings and discusses the limitations and advantages of such methodologies for further understanding alcohol problems in a community.

Adolescent↗

Assessing bias in community-based prevalence estimates: towards an unduplicated count of problem drinkers and drug users.

General population survey estimates of the overall prevalence of problem drinking and drug use in a community are biased by the exclusion of non-household populations. Estimates based on compiling prevalences in community institutions may also be biased due to over-counting of users of more than one institution. This paper examines prevalence estimates derived from probability samples of problem drinkers in the general population and within alcohol treatment, drug treatment, mental health, criminal justice and welfare agencies in a single US county. Data sets are merged and weighted to reflect a community sample of institutions, and a 17% subset of cases is identified within the institutional samples that are not living in housing units typically included in general population sampling frames. The difference in prevalences of problem drinking in the household and non-household populations is found to be large: 11% and 48%, respectively. Even greater differences are found between estimates of unprescribed weekly drug use (6% and 47%, respectively) and combined problem drinking and weekly drug use (2% and 27%, respectively). This suggests that confining samples to the household population can systematically under-represent the prevalence of problem drinking and drug use. A second source of bias in prevalence is characteristic of studies using records from multiple institutions. When duplication of service use in the five agency samples is considered, it becomes apparent that prevalence may be biased upward due to over-counting of multiple service users.

Adolescent↗

Infertility, involuntary infecundity, and the seeking of medical advice in industrialized countries 1970-1992: a review of concepts, measurements and results.

Several new methods of infertility treatment have been developed. There is therefore an increasing interest among physicians in gaining knowledge about the occurrence of infertility in order to estimate the need for treatment. This article examines epidemiological studies in industrialized countries 1970-1992 on the prevalences of infertility, involuntary infecundity, and the seeking of medical advice for infertility. The studies are compared on the basis of study design, definitions, methods of measurement and results. Comparison of the studies is difficult, as researchers use different concepts and there is a large variation in the delineation of the populations involved. The current prevalence of infertility among women in the fertile age group varies from 3.6 to 14.3%, the life-time prevalence varies from 12.5 to 32.6%, the prevalence of involuntary infecundity from 2.6 to 5.9%, and medical contact for infertility from 3.6 to 17%. It is probable that these large differences in prevalences are mainly due to differences in definitions and methods of measurement. We recommend that future studies be carried out in representative population groups, and that only couples who have tried to have children should be included in the estimated prevalences.

Developed Countries↗

Infertility and the seeking of infertility treatment in a representative population.

OBJECTIVE: To investigate lifetime prevalence of infertility, the seeking of infertility treatment and outcomes of treatment. DESIGN: Cross-sectional postal questionnaire study. SETTING: County of Copenhagen, Denmark. SUBJECTS: Three thousand, seven hundred and forty-three women, 15 to 44 years old, selected at random were asked about infertility, their seeking of infertility treatment, diagnoses provided by their doctors and subsequent parenthood. Response rate was 78%, n = 2865. A random sample of non-responders was interviewed by telephone. MAIN OUTCOME MEASURES: Fertility status, seeking of infertility treatment, subsequent deliveries and adoptions. RESULTS: Of the women who had attempted to have a child, 26.2% had experienced infertility; 4.1% of the women aged 25 to 44 years were currently primarily infertile and 8.6% had involuntarily not delivered a first child; 47.4% of the infertile women had sought infertility treatment. Significant predictors for seeking infertility treatment were school education > 9 years and not having delivered a child. Of the infertile women 54.9% subsequently had a child. Only 30% of these reported that the successful delivery was treatment-related. CONCLUSIONS: The health care system should see to it that infertile couples from lower social classes are offered information on the possibility of infertility treatment. High quality infertility treatment has to include both the "supply" of taking care of the infertile couple's psychosocial strain and the goal of ensuring successful pregnancies.

Adolescent↗

Recalled menarche in relation to infertility and adult weight and height.

BACKGROUND: Possible relationships between menarche and fertility and fecundity later in life have not been fully clarified. METHODS: A cross-sectional study was made in a random sample of 3743 women, aged 15-44 years, in the country of Copenhagen; 78% responded. Telephone interviews were made with a sample of the non-respondents. RESULTS: Associations were observed between early menarche and increased risk of pelvic inflammatory disease (odds ratio 2.4 (1.4-4.1)) and spontaneous abortion (OR 3.1 (1.6-5.7)) later in life. On the other hand, no association was found with irregular bleedings or amenorrhoea; nor was a significant relationship established with fertility or fecundity. Late menarche was associated with underweight (OR 3.1 (1.4-6.9)), and early menarche with excess weight (OR 5.0 (2.4-10.6)). A significant positive correlation (R = 0.17) was found between menarcheal age and final body height. CONCLUSIONS: Menarche was not associated with fertility or fecundity. Early menarche was associated with pelvic inflammatory disease and spontaneous abortion. Menarche showed relationships with weight and height.

Adolescent↗

The advanced registered nurse practitioner in rural practice.

Availability of health care to rural areas is limited. FNP's in rural practice have the ability to address rural needs and tailor interventions to the specific attributes of rural populations. The objective of this article is to define the role and the functions of the FNP and to support and defend this role in the rural setting. Much of the FNP research presented in the literature to date focuses on urban populations; therefore, the unique health care problems and needs of rural populations will first be defined as they relate to the FNP's scope of practice.

Humans↗