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Biomedical subjects

L Sebnem Türkün

Publications and source records attributed to L Sebnem Türkün.

12 recordsLinked to original sources
PubMed2006

Bactericidal effect of Er,Cr:YSGG laser on Streptococcus mutans.

The aim of this study was to compare the antibacterial activities of Er,Cr:YSGG laser with two different power outputs against a chlorhexidine gluconate-based cavity disinfectant. A cavity tooth model test was used to determine the antibacterial activity. Four cylindrical cavities were prepared on the dentin surface of 10 bovine incisors and left in contact with Streptococcus mutans for 72 hours to allow bacterial invasion. Following which, Er,Cr:YSGG laser with 0.75 W and 1 W power outputs and a chlorhexidine gluconate-based cavity disinfectant were applied separately on one of the three infected cavities, whereas the fourth was left untreated for control. Standardized amounts of dentin chips were obtained from the cavity walls, and the number of bacteria recovered was counted. Statistical analysis was carried out using one-way ANOVA and Dunnett's C test (p=0.05). No significant differences were observed among the data obtained from the chlorhexidine gluconate-based cavity disinfectant and the two Er,Cr:YSGG laser groups (p>0.05). However, when compared to the control group, both Er,Cr:YSGG laser groups and the chlorhexidine gluconate-based cavity disinfectant resulted in significantly less bacterial recovery (p<0.05). In conclusion, the antibacterial activity on S. mutans demonstrated by Er,Cr:YSGG laser with both energy outputs was similar to that of the tested chlorhexidine gluconate-based cavity disinfectant.

Animals↗
PubMed2006

Is an antibacterial adhesive system more effective than cavity disinfectants?

PURPOSE: To compare the antibacterial activity of an adhesive system containing an antibacterial monomer MDPB, Clearfil Protect Bond with three different cavity disinfectants, chlorhexidine gluconate-based Consepsis, benzalkonium chloride-based Tubulicid Red and 3% hydrogen peroxide. METHODS: Materials were tested using agar well technique and a tooth cavity model. The test materials were filled in the agar wells of plates inoculated with Streptococcus mutans. After 48 hours of incubation, the zones of inhibitions were measured in millimeters. For the tooth cavity model test, cylindrical cavities were prepared in the flat occlusal dentin of human extracted molars. The teeth were left in a broth culture of Streptococcus mutans at 37 degrees C for 72 hours allowing bacteria to invade. Teeth were then randomly assigned into five groups of five teeth (10 cavity preparations) each. In the first four groups test materials were applied into the cavities following the manufacturer's instructions and the cavities in the fifth group were left untreated for control. The teeth were kept in saline for 72 hours. Standard amounts of dentin chips were obtained from the cavity walls and the number of bacteria recovered was counted. RESULTS: The results were analyzed by ANOVA, Dunnett C and Bonferroni tests. For the agar well technique, Clearfil Protect Bond primer exhibited greater inhibition zones than all three cavity disinfectants (P< 0.05). When tested by the cavity method, the application of Clearfil Protect Bond system resulted in significantly less bacterial recovery than all disinfectants (P< 0.05). For both microbiological methods, there were no significant differences between the antibacterial activities of Consepsis and Tubulicid Red (P> 0.05). They were superior to hydrogen peroxide in the cavity test method (P< 0.05).

Analysis of Variance↗
PubMed2006

Shear bond strength of different adhesives to Er:YAG laser-prepared dentin.

PURPOSE: The aim of this study was to examine the influence of Er:YAG laser on the shear bond strength of three different adhesives to lased dentin. MATERIALS AND METHODS: Seventy specimens obtained from 35 extracted human molars were embedded in polyester resin and ground with silicon carbide papers. The samples were divided into seven groups. 1. Er:YAG laser (Key Laser 3, KaVo) + Clearfil Protect Bond (Kuraray); 2. Er:YAG laser + Clearfil tri-S Bond (Kuraray); 3. Er:YAG laser + 37% H3PO4 + Single Bond 2 (3M-ESPE); 4. Er:YAG laser + Single Bond 2; 5. conventional method + Clearfil Protect Bond; 6. conventional method + Clearfil tri-S Bond; 7. conventional method + 37% H3PO4 + Single Bond 2. The samples were subjected to shear bond testing 24 h after bonding. Statistical analyses were carried out by two-way ANOVA, t-test, one-way ANOVA, post-hoc Tukey's and Dunnett C test (p = 0.05). RESULTS: Only the Er:YAG laser + Clearfil tri-S Bond group demonstrated significantly higher bond strengths vs conventionally prepared specimens (p < 0.05). There were no significant differences between the shear bond strengths of Single Bond 2 adhesive applied to laser- vs bur-treated specimens (p > 0.05). In laser prepared samples, Clearfil Protect Bond showed the highest scores (p < 0.05), whereas in conventionally prepared groups, no statistical differences were observed between Clearfil Protect Bond and Clearfil tri-S Bond (p > 0.05). CONCLUSION: Er:YAG laser irradiation did not adversely affect the shear bond strength of Single Bond 2 and Clearfil Protect Bond to dentin, whereas it increased the shear bond strength values of Clearfil tri-S Bond.

Acid Etching, Dental↗
PubMed2006

Stain retention and surface characteristics of posterior composites polished by one-step systems.

PURPOSE: To compare susceptibility to stain of three posterior composite surfaces polished with two one-step polishing systems, to test the efficiency of the re-polishing to remove the stain formed and to analyze the polished surfaces by SEM. METHODS: The resin composites tested were Clearfil Photo Posterior, SureFil and Filtek P60. The polishing systems used were Sof-Lex Brush and PoGo. Twenty-two discs of each material were fabricated and divided into two treatment groups of 11. Out of the latter, six were used for SEM evaluation for surface morphology and five for stain retention testing. The discs were roughened with silicon paper and polished for 30 seconds with each system. Specimens were immersed in coffee and distilled water for 7 days. Color measurements were made at baseline and after 1, 3, 5, and 7 days with a spectrophotometer. The specimens were then re-polished with the same systems and their colors re-measured. All comparison of color change and re-polishing were subjected to repeated measures ANOVA with a Bonferroni post hoc test (P < or = 0.05). RESULTS: After 1 week, the coffee stained all the resin composites. Clearfil Photo Posterior was the least stained composite while Filtek P60 was the most. Regardless of the materials, PoGo polishing was less susceptible to staining (P < or = 0.05). The color change could be partially removed with re-polishing. The surfaces of Sof-Lex Brush polished samples, except the Clearfil group, were scratched and thus more susceptible to stain retention.

Analysis of Variance↗
PubMed2005

Effect of an antibacterial adhesive on the bond strength of three different luting resin composites.

OBJECTIVES: Effect of a dentin adhesive system containing antibacterial monomer-MDPB (Clearfil Protect Bond) on the shear bond strength of all-ceramic-IPS Empress 2 restorations luted with three different dual-polymerizing systems (Variolink 2, RelyX ARC and Panavia F 2.0) to dentin was investigated. METHODS: One hundred and eight all-ceramic discs (2 x 3mm; IPS Empress 2) were fabricated and ultrasonically cleaned. The buccal surfaces of 108 non-carious extracted human premolars were flattened to expose dentin and subsequently polished with 600-grit wet silicon carbide paper. Three dual-polymerizing luting systems had test groups and control groups consisting of 18 samples each. For the test groups Clearfil Protect Bond was applied to the exposed dentin surfaces. Control groups received the original bonding procedures of each adhesive system. After the all-ceramic samples were luted to the teeth, thermocycling was performed 5000 times. Shear bond strengths were tested using Shimadzu Universal Testing Machine until failure. Analysis of fractured dentin surfaces were performed using Optical Microscope at x10 and x1000 magnifications and the images were analyzed with Image Analyzer. Data was analyzed with one-way ANOVA and Bonferroni test at a significance level of p<0.05. RESULTS: Mean shear bond strength data of the groups in MPa were; Variolink: 20.45+/-4.75, Variolink+Clearfil Protect Bond:29.32+/-2.37, RelyX ARC:18.82+/-3.19, RelyX ARC+Clearfil Protect Bond:25.58+/-4.05, Panavia F 2.0:17.11+/-2.98, Panavia F 2.0+Clearfil Protect Bond:24.40+/-7.46. Application of the antibacterial adhesive increased the shear bond strengths of all three dual-polymerizing systems to dentin (p=0.00). The surface analysis showed that most of the specimens showed the adhesive failure mode between the dentin and the composite luting agent interface. CONCLUSION: The antibacterial adhesive system Clearfil Protect Bond can be safely used to prevent the potential risk of complications resulting from bacterial activity regardless of affecting the bond strength of IPS Empress 2 restorations luted with the dual-polimerizing systems used in this study.

Anti-Bacterial Agents↗
PubMed2005

The clinical performance of one- and two-step self-etching adhesive systems at one year.

BACKGROUND: The author compared the clinical performance of a two-step self-etching adhesive system and a one-step self-etching adhesive system over one year. METHODS: Thirty-five patients with noncarious cervical lesions were enrolled in the study. The author restored 163 lesions using a two-step (Clearfil Protect Bond, Kuraray, Osaka, Japan) or a one-step (Xeno III, Dentsply/DeTrey, Konstanz, Germany) self-etching adhesive system. Enamel margins were not beveled, and no mechanical retentions were placed. The author evaluted the restorations at baseline and at three, six, nine and 12 months after placement using modified Ryge criteria for color-matching ability, marginal discoloration, marginal adaptation, initial caries formation, anatomical form, postoperative sensitivity and retention loss. RESULTS: The author assessed the changes in the parameters using the Cochran Q test and the McNemar test at a significance level of .05. At one year, the retention rates for the restorations in the two-step group were 100 percent; they were 96 percent for the restorations in the one-step group. Of the retained 75 restorations from the one-step group, two had marginal discoloration and slight anatomical form problems. In both groups, color-matching ability and postoperative sensitivity remained excellent. CONCLUSION: The performance of both self-etching adhesive systems was excellent during this one-year clinical trial. However, the two-step system exhibited slightly better retention than the one-step system. CLINICAL IMPLICATIONS: The one- and two-step self-etching adhesive systems evaluated in this study provided excellent clinical retention in noncarious lesions without mechanical retention.

Acid Etching, Dental↗
PubMed2005

Clinical performance of a packable resin composite for a period of 3 years.

OBJECTIVE: The purpose of this clinical study was to evaluate SureFil packable resin composite for posterior restoration of permanent teeth for a period of 3 years. METHOD AND MATERIALS: Fifty-five resin composite restorations were placed in 36 patients (16 Class I and 39 Class II restorations). After cavity preparation, the enamel was etched with 34% phosphoric acid. Prime & Bond NT was applied 20 seconds to dentin and etched enamel and cured for 20 seconds. The teeth were restored in 3- to 5-mm increments. The restorations were assessed after placement, at 6 months, 1 year, 2 years, and 3 years for color stability, marginal discoloration, marginal adaptation, secondary caries, surface texture, anatomic form, and postoperative sensitivity according to Ryge's criteria. The changes in the parameters were assessed with Friedman test analysis with a Bonferroni correction at a significance level of .05. RESULTS: Forty of the monitored 47 restorations were classified as excellent after 3 years. Thirty-one restorations were graded Bravo at baseline for color match. At the 3-year assessment (n = 47) the color of the 31 restorations had not changed. Two restorations (same patient) were lost after 1 month and were scored as Charlie until the end of the study. After 3 years there were five Bravos and one Charlie with marginal discoloration, five Bravos with marginal adaptation, and three Bravos with anatomic form (P < .05). CONCLUSION: After 3 years of clinical service, SureFil packable resin composite, with a failure rate of 6%, was considered to be successful in Class I and II restorations.

Adult↗
PubMed2005

Antibacterial activity of two adhesive systems using various microbiological methods.

PURPOSE: To compare the antibacterial activities of two dentin bonding systems (DBS), Clearfil Protect Bond and Xeno III, by agar well, paper and dentin disks, and a cavity tooth model. MATERIALS AND METHODS: For the well technique, the test materials were filled in the agar wells inoculated with Streptococcus mutans (ATCC 25175). The paper disks were embedded in adhesives and placed on the seeded agar plates for the second technique. The adhesives were applied on the dentin disks and placed in holes in the plates for the third technique. After 48 h, the zones of inhibition were measured. In the cavity tooth model test, 3 cavities were prepared in the flat occlusal dentin of extracted human molars. The teeth were left in S. mutans for 72 h to allow bacterial invasion. The DBS were applied in the same manner as in clinical application on each of the two infected cavities and the third was left unapplied for control. The teeth were kept in saline for 72 h. Standard amounts of dentin chips were obtained from the cavity walls and the number of bacteria recovered was counted. RESULTS: The results were analyzed by factorial ANOVA and Dunnett C test. Clearfil Protect Bond primer exhibited the greatest inhibition zones followed by Consepsis and unpolymerized Xeno III in all the techniques tested (p < or = 0.05). Clearfil Protect Bond resulted in significantly less bacterial recovery than Xeno III by the tooth cavity method (p < or = 0.05). CONCLUSION: Clearfil Protect Bond was found to be the most antibacterial material with all the techniques used. Furthermore, Clearfil Protect Bond was able to inactivate the bacteria in the cavity more effectively than Xeno III.

Anti-Bacterial Agents↗
PubMed2004

Effect of bleaching and repolishing procedures on coffee and tea stain removal from three anterior composite veneering materials.

UNLABELLED: Discolored teeth can be treated with resin veneers, but their color changes when confronted with staining solutions. Polishing procedures can provide a remedy for highly stained composites, but they tend to remove some materials as well. However, bleaching procedures are an effective, nondestructive method for solving the problem. The aim of this study was to compare the color change of three veneer composites exposed to staining solutions and to evaluate the effectiveness of a 15% hydrogen peroxide bleaching agent and three polishing systems to remove the stain. Forty-five disks (12 x 2 mm) each of Clearfil ST (Kuraray Co. Ltd., Osaka, Japan), Esthet-X (Dentsply/Caulk, Milford DE, USA), and Filtek A110 (3M ESPE, St. Paul, MN, USA) were prepared. The specimens were polished with Sof-Lex (3M ESPE), Enhance (Dentsply/Caulk), or PoGo (Dentsply/Caulk). Five specimens for each material-polishing system combination were immersed in coffee (Nescafe Classic, Nestle SA, Vevey, Switzerland) or tea (Earl Grey, Lipton, Blackfriars-London, England) for 7 days. The remaining disks were stored in water. Color measurements were made with a spectrophotometer (X-Rite Seroice SP78, Loaner, Köln, Germany) at baseline; after 1, 3, 5, and 7 days; and after bleaching and repolishing. After 1 week, one side of the specimens was bleached with Illuminé-office (Dentsply De Trey GmbH, Konstanz, Germany) for 1 hour, and the other side was repolished for 30 seconds. All comparisons of color change for the polishing systems, times, and staining solutions were subjected to repeated measurements of analysis of variance. Paired t-test was used to examine whether significant color differences (deltaE*) occurred during immersion at the specified time intervals (p < or = .05). Filtek A110 was the least stained resin composite. Its color remained under a deltaE* value of 2 during the study. Clearfil ST exhibited the most color change after 1 week. All specimens polished with Enhance showed less staining, whereas those polished with the Sof-Lex system demonstrated the most color change. Water did not cause a variance in the deltaE*. There was no difference in the staining potential of coffee and tea. Bleaching and repolishing were effective in removing the stains. The resin composites tested reversed nearly to baseline color with the bleaching and to less than values at 1 day of staining with repolishing. The coffee and tea brands tested stained the composites used in this study equally. In-office bleaching was found to be more effective than repolishing in the restitution of the color. CLINICAL SIGNIFICANCE: The results of this study suggest that the discoloration of resin veneers can be partially removed by in-office bleaching and repolishing procedures.

Coffee↗
PubMed2004

Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins.

OBJECTIVES: The purpose of this study was to determine the effect of three cavity disinfectants (chlorhexidine gluconate-based, Consepsis; benzalkonium chlorite-based, Tubulicid red; iodine-potassium iodide/copper sulphate-based, Ora-5) on the microleakage of nonrinsing dentin-bonding systems, Clearfil SE Bond and Prompt L-Pop. METHOD AND MATERIALS: Class V cavity preparations were placed on the buccal and lingual surfaces of extracted molars with occlusal margins at the enamel and gingival margins in cementum. In the experimental groups, cavities were treated with combinations of one of the three cavity disinfectants with either Clearfil SE Bond or Prompt L-Pop. The preparations without disinfectant application were used as the negative controls for each adhesive system, and the cavities in which neither disinfectant nor dentin-bonding resin were applied, served as the positive controls. After the cavity preparations were restored with resin composite, specimens were thermocycled, stained, and sectioned to evaluate dye penetration. The tooth-resin composite interface of the sectioned specimens was examined under scanning electron microscopy. RESULTS: Consepsis and Tubulicid red did not significantly affect the sealing ability of Clearfil SE Bond and Prompt L-Pop. Ora-5 exhibited gap formations at the tooth-resin composite interface and produced significantly higher microleakage when used with these bonding systems. CONCLUSION: Consepsis and Tubulicid red can be used as cavity disinfectants with Clearfil SE Bond and Prompt L-Pop without affecting their sealing abilities. However, Ora-5 is not an appropriate disinfectant to use with these bonding systems.

Anti-Infective Agents, Local↗
PubMed2003

Two-year clinical evaluation of a packable resin-based composite.

BACKGROUND: Packable resin-based composites were introduced in 1998, but few clinical studies have been conducted to evaluate them. The authors conducted a clinical study to determine the two-year performance of SureFil (Dentsply DeTrey GmbH, Konstanz, Germany) packable posterior resin-based composite in Class I and II restorations. METHODS: An operator (L.S.T.) restored 55 cavities in 36 patients (16 Class I restorations and 39 Class II restorations). After cavity preparation, she etched the enamel with 34 percent phosphoric acid, applied Prime & Bond NT (Dentsply DeTrey GmbH) to dentin and etched enamel for 20 seconds and then cured it for 20 seconds. She restored the cavity using 3- to 5-millimeter increments of SureFil. Independent examiners assessed the restorations after placement and at six months, one year and two years for color matching, marginal discoloration, marginal adaptation, secondary caries, surface texture, anatomical form and postoperative sensitivity, using the Ryge criteria. RESULTS: The authors assessed the changes in the parameters during the two-year period using a software program with Friedman test analysis with a Bonferroni adjustment at significant level of P = .05. At baseline, 31 restorations were graded as Bravo for color match. At the six-month and one-year recall periods (n = 55), 53 restorations remained unchanged. Two restorations from the same patient fell out after one month. After two years (n = 50), there were five Bravos for surface staining and three for marginal adaptation (P < .05). CONCLUSION: After two years of clinical service, SureFil packable resin-based composite had a success rate of 96 percent, and the authors considered it successful in Class I and II restorations. CLINICAL IMPLICATIONS: SureFil packable resin-based composite can be successful in clinical situations with limited-sized cavities and proper application of restorative techniques.

Acid Etching, Dental↗