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Biomedical subjects

L Spångberg

Publications and source records attributed to L Spångberg.

At least 19 recordsLinked to original sources
PubMed1995

Transdentinal stimulation of reparative dentine formation by osteogenic protein-1 in monkeys.

Recombinant human osteogenic protein-1 (OP-1) when applied to freshly cut dentine stimulated significantly more reparative dentine than calcium hydroxide paste in permanent monkey teeth. The response to OP-1 was dependent upon the concentration applied to the tooth as a cavity liner as well as the thickness of the residual dentine. The response to calcium hydroxide was similarly dependent upon the thickness of the residual dentine. These data suggest that dental pulps contain cells, including perhaps mature odontoblasts, responsive to OP-1. Therefore OP-1 may be useful in the therapeutic induction of reparative dentine formation.

Analysis of Variance↗
PubMed1994

The time-course of the induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1.

Recombinant human osteogenic protein-1 (hOP-1, BMP-7) induces cartilage and bone formation when implanted in extra- and intraskeletal sites in vivo. rOP-1 also preserves pulp vitality and stimulates reparative dentine formation when placed on partially amputated vital dental pulp tissue. The amount of dentine formed in 6 weeks was earlier found to be proportional to the total amount of rOP-1/carrier placed on the pulp and the capacity of the pulp to respond to rOP-1 appeared to be independent of the amount of coronal pulp removed. This reparative dentine was not completely mineralized after 6 weeks healing. Experiments were now made to determine the capacity of hOP-1 to preserve the vitality of and induce reparative dentine in vital radicular pulps. The extent of tissue mineralization present after 1, 2, 4 and 6 months' healing time in permanent monkey teeth was assessed. Radicular pulp vitality was maintained, reparative dentine formed, and mineralization was nearly 75% complete after 1 month and more than 95% after 4 months. The effects of irrigating the exposed pulps with EDTA, sodium hypochlorite or saline were also compared. Significantly more sodium hypochlorite-treated pulps became non-vital and the root canals of all the non-vital teeth contained bacteria at the time of sacrifice.

Animals↗
PubMed1992

Dentin as inhibitor of bacterial toxicity on pulpal cells in vitro.

Many studies have shown that the major cause of pulpal disease is the presence of bacteria or their by-products in the dentinal tubules. The purpose of this investigation was to develop an in vitro model, simulating the pulp chamber, that would permit the study of the transport of bacterial by-products through dentin and their effect on pulpal cells. Human pulpal cells were cultured in a modified Sykes-Moore chamber and exposed through dentin to sonicated extracts of Porphyromonas gingivalis ATCC 33277. The cell response was evaluated with the thymidine incorporation method. The results were compared with the cell response obtained after direct exposure to the same irritant. It was found that dentin significantly restricts the diffusion of bacterial proteins in a 24-h experimental period. The time needed for the first bacterial protein molecule to cross the dentin barrier was 6 h. The "diffusion velocity" of the bacterial proteins was 0.023 microns/s. The proposed model has further applications in biocompatibility and microleakage research.

Bacterial Proteins↗
PubMed1991

[HIV-infected drug addicts in a clinic for infectious diseases are dissatisfied and desire more treatment].

In structured interviews with 171 HIV-positive iv drug abusers at the HIV clinic of Roslagstull Hospital in Stockholm, were investigated their current drug abuse and any wishes they might have as to their treatment. Over 75 per cent reported drugs abuse in the past 12-month period-chiefly iv heroin. Half of them reported that they lacked any form of counselling for their addiction, and something over 40 per cent wanted treatment- the predominant choice being methadone treatment. Despite methadone treatment having been found effective in cases of opiate-dependence, and in many cases a prerequisite of rehabilitation, the queues for it are very long in Sweden--up to two years. Adequate treatment for these patients needs to be made more rapidly available, and greater attention paid to the patients' own views. This is of the utmost importance, both epidemiologically and from the individual patient's point of view, and is a prerequisite in preventing the spread of HIV infection.

Adult↗
PubMed1991

The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing.

The antibacterial effect of calcium hydroxide as a short-term intracanal dressing was clinically evaluated by applying the medicament for 10 minutes or 7 days in root canals of teeth with periapical lesions. The results showed that the 7-day dressing efficiently eliminated bacteria which survived biomechanical instrumentation of the canal, while the 10-minute application was ineffective.

Bacteria, Anaerobic↗
PubMed1990

Screening for HIV in pregnant women: a study of maternal opinion.

Pregnant women residing in the metropolitan area of Stockholm were screened for HIV in a 12-month pilot programme. The acceptance rate was 99.3%. Three HIV-antibody-positive women (0.04%) were identified. The screening was followed up by a study designed to ascertain the attitudes of the women towards the test. The vast majority had a positive attitude to HIV screening out of consideration for the health of the expected child. One out of seven felt uneasy while awaiting the test result. Significantly more women among those who thought repeatedly about the test had already considered having an HIV test before pregnancy. Once the women found out that their HIV test was negative it did not affect their subsequent experience of pregnancy.

AIDS Serodiagnosis↗