[Cariogenicity of acidogenic and aciduric streptococci (S. mutans) in a rat experiment].
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Biomedical subjects
Publications and source records attributed to L Stösser.
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Changes of dental plaque were investigated in Osborne-Mendel-rats under the influence of a sucrose-containing diet as well as the inhibition of it by antibiotics (streptomycin, erythromycin, penicillin) in order to find out the optimal conditions of animal association with Str. mutans (OMZ 176). The cariogenic diet did not alter the isolated total germ numbers, but modified the flora to a streptococcal one. The application of an erythromycin/penicillin mixture caused a 100 fold decrease of the germ number and streptococcal elimination so that an association of the germ Str. mutans detectable by microbiological methods could take place at this moment. The obtained caries scores differed from the non-contaminated group. Mono-associated gnotobiotic Wistar rats developed more carious lesions than conventional animals of the same strain; therefore Str. mutans OMZ 176 was classified as high cariogenic.
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A study has been made to determine the inhibitory action upon the progress of dental caries of two trace elements (fluorine and molybdenum) combined with chlorohexidine with the goal of increasing the efficiency of fluoride treatment. However, the results obtained show that the chosen combination of trace elements did not lead to a substantial increase in cariostatic action. Combined use of fluorides and chlorohexidine was seen to exert an inhibitory action on the progress of dental caries.
In two caries model experiments on Wistar rats, a significant reduction in caries was observed following local application of Fluorprotektor and Duraphat. There appears to be no difference between the caries-protective properties of these two agents. The accumulation of fluoride in the superficial enamel was tenfold to elevenfold for the two lacquers. Residual Fluorprotektor was found on teeth even after twenty-four days from the application thereto.
The comparative testing of the tooth-pastes "Silca mit Fluor", "Fluorodent" and "Silica" (without fluorine) in a programmed animal experiment for 6 weeks revealed differences in caries-protective efficiency and and in deposition of fluoride in dental enamel, "Silca mit Fluor" being superior to "Fluorodent".
The effect of stepwise sugar reduction in cola drinks was tested in a programmed animal experiment using Wistar rats. In a parallel study the pH-decrease at different sucrose concentrations was measured in vitro using a plaque model system which involved s. mutans (strain OMZ 176). The reduction of sugar contents by 20%, 33% und 42% results in a lower acid production by s. mutans strain connected with a decrease of the experimental produced caries. For this reason the sugar restriction in cola drinks will be seen as a contribution towards an improvement of dental health.
Ca-45 was injected Wistar-rats during pregnancy that receive destillated or with 100 ppm F- enriched water. The effect of fluoride on transport of calcium through placenta was calculated from results of Ca-45-activity of fetuses. Besides results were obtained about fluoride- and Ca-45-uptake of mothers femur and about the development of rats under fluoride ingestion