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L Sula

Publications and source records attributed to L Sula.

At least 19 recordsLinked to original sources
PubMed1984

Lytic potency against various mycobacterial strains of the phage isolated from Mycobacterium leprae murium "Douglas".

The lytic potency of a newly isolated phage Al-1 obtained from the laboratory strain M. leprae murium "Douglas" was examined. The phage was multiplied on the laboratory strain M. smegmatis ATCC 607 and for the lytic test 0.1 ml of suspension containing PFU 10(5) was used. In the whole 18 mycobacterial strains both slowly and fast growing multiplied in liquid Sula's medium were tested. For phage lytic tests two simple agar media and standard Redmond's medium RVA-24 were used. The examined slowly growing mycobacteria (H37Rv. M. bovis "Ravenel", M. avium "Kirchberg", M. kansasii "Svizenský", M. tbc INH resist., M. tbc INH, STM, PAS resist.) were resistant to the tested phage similarly as M. phlei from the group of fast growing strains. The results of phage tests on all three used media were characterized by a confluent phage lysis with the exception of the strain M. butyricum "Rabinovic", in which even on a very rich media an incomplete lysis with countable plaques was found out. The use of the phage Al-1 for the phage typification also of the strain M. leprae murium is considered on the basis of the inhibition growth tests on Ogawa's egg media.

Culture Media↗
PubMed1984

Isolation of mycobacterial phage from the laboratory strain Mycobacterium leprae murium "Douglas".

The colony microstructure of the laboratory strain Mycobacterium leprae murium "Douglas" cultivated on Ogawa's egg medium was examined. A bioptical sample from the liver of a white mouse subcutaneously infected and observed for ten months was used as inoculum. The inoculum contained 5.2 X 10(9) acidfast rods. The Ogawa's media were incubated in 5% atmosphere of CO2 at 33 degrees C to 37 degrees C for 6 to 10 months. The outgrown colonies were killed with a formol solution, then embedded into the agarparaffin and cut out with the aid of Reichert's microtom. In thin sections there was an apparent vacuolisation of colonies proving the presence of the temperate phage, which was isolated from the bacterial suspension inoculated on the host non lysogenic strain Mycobacterium smegmatis ATCC 607. On the simple agar medium N-4 the number of 2.4 X 10(9) living particles was achieved, which shows the possible use of this phage for differential diagnostic purposes in the taxonomy studies of mycobacteria.

Animals↗
PubMed1983

[Incidence of phagotypes of Mycobacterium tuberculosis in Czechoslovakia and East Germany and of Mycobacterium bovis in Czechoslovakia and Argentina].

The following study gives review of the results of the phagetyping of M. tuberculosis strains in different localities of Czechoslovakia and German Democratic Republic (165 strains from Czechoslovakia and 102 strains from GDR) and the comparison of the occurrence of phage subgroups of M. bovis strains in two different localities of Czechoslovakia (62 strains) and Argentina (93 strains). The results of the phagetyping of the Czechoslovak M. tuberculosis strains show that this group comprises all the 6 phage subgroups and agree very well in subgroups Ax, A2, B and C with the M. tuberculosis strains from GDR. Both groups of strains differ in subgroups A2 (9) and D. The results with the fresh isolated Argentinian M. bovis strains show that this group has only subgroups Ax and D in comparison with the old laboratory strains of M. bovis which have subgroups Ax, A2(9), and D. The phagetyping of the M. tuberculosis and M. bovis strains was undertaken with the help of 11 international mycobacterial phages. The use of mycobacterial phages in 1 RTD (Routine Test Dilution) and 10 RTD concentrations (in contrary to the 1 RTD and concentrated phage suspensions used previously) decreased the number of the "unspecific" reactions.

Argentina↗
PubMed1982

The microscopical pattern of growth of Mycobacterium leprae-murium "Douglas" in microcolonies.

A thin section technique of Ogawa egg yolk culture medium inoculated with Mycobacterium leprae-murium was found to reveal microscopical growth of the strain which could not be demonstrated by macroscopical examination. A peculiar structure of the growth, characterized by many lytic spots different in size, was observed indicating the possible presence of a temperate phage which may interfere with the synthesis of nucleic acids needed for the active multiplication of M. leprae-murium.

Animals↗
PubMed1981

The colonial microtexture of human mycobacterial strains isolated in Burma.

Sixteen strains of Mycobacterium tuberculosis isolated in Burma were investigated for microcolonial texture using the thin section technique developed at the WHO Collaborating Centre in Prague. The strains were grown in deep (Sula's liquid medium) and surface cultures (Loevenstein-Jensen medium). Their colonies killed by 10% formol were embedded in 2% agar and paraffin, cut by a Reichert microtome and stained by the Ziehl-Neelsen technique. According to the surface texture and deep growth pattern, the Burmese strains were divided in three different groups. The European type was characterized by a growth strictly confined to the surface of the Loevenstein-Jensen medium, the African type by combined surface and subsurface growth, and the Burmese type not seen in Europe and Africa by non acid-fast granular and acid-fast colonies with distinct formation of strongly acid-fast cords both in surface and subsurface growth.

Bacteriological Techniques↗
PubMed1979

Accidental contamination of diagnostic cultures of mycobacteria and phage identification of the contaminating strain.

Phage typing of mycobacteria is a new technique not yet widely used for classification and intraspecies differentiation. Systematic long-term studies organized by the WHO have succeeded, using a battery of 11 different mycobacterial phages, in dividing M. tuberculosis into 3 different phage sub-groups, preliminarily labelled as A, B and C. A special case of phage-typing is presented which enabled identification of a virulent mycobacterial strain causing accidental contamination of diagnostic mycobacterial cultures. The strain was an old laboratory one, H37Rv, belonging to phage subgroup B.

Bacteriophage Typing↗
PubMed1979

Self-inoculation with Mycobacterium tuberculosis Weiszfeiler W-115.

After intracutaneous self-inoculation with 0.05 mg (wet weight) of Mycobacterium tuberculosis W-115 containing approximately 600 000 viable units, the local reaction was observed during a period of 9 weeks. No adverse effects or general reaction were observed. From the lesion the W-115 strain was recultivated. It was labelled W-115(78)ST vaccine strain and is used for further studies. On the basis of experiments on laboratory animals, monkeys, human new-borns and adults, the strain W-115 is considered as safe for antituberculous vaccination as the strains Mycobacterium bovis BCG and the attenuated "MP" strain of Mycobacterium microti OV 166.

BCG Vaccine↗
PubMed1977

Fatal generalized BCG histiocytosis.

An infant was vaccinated at the age of 3 days with BCG vaccine. At the age of 3 years 10 months he developed an infection by Salmonella typhimurium. The infection persisted with recurrent episodes of fever, peri-nephritic abscess, abscesses of lymph nodes, hepatomegaly, splenomegaly and paravertebral and retro-peritoneal abscesses, from which Salmonella were isolated. At the age of 6 years and 2 months later. At post-mortem examination there were widespread histiocytic nodules in many organs, from which Mycobacterium bovis BCG were cultured. One previous case has been reported from Czechoslovakia. The mother of that child was the sister of the father of the child reported here. It was not possible to investigate the possibility of abnormalities of humoral or cellular immunity in the family.

BCG Vaccine↗
PubMed1975

World Health Organization studies on bacteriophage typing of mycobacteria. Subdivision of the species Mycobacterium tuberculosis.

The ability of lytic mycobacteriophages to subdivide the species Mycobacterium tuberculosis reliably has been studied using a series of 100 strains isolated from cases of tuberculosis in the Netherlands. Techniques for the propagation and application of the viruses have been standardized, as have the conditions for growth and preparation of bacterial strains. On the basis of lytic results with 11 mycobacteriophages, it is proposed that the species Mycobacterium tuverculosis may be subdivided into at least 3 major phage types, A, B, and C, and into 2 subjects, Ax and A2. The reliability of the individual bacteriophage lytic result has been assessed, and the relationship between phage reliability and the degree of certainty with which a strain may be assigned to a phage type is described. The effect of rigorous standardization of techniques on the reliability of bacteriphage typing is demonstrated, and a standard protocol is proposed.

Bacteriophage Typing↗
PubMed1975

Ultrastructure of rabbit lung intravenously infected with Mycobacterium simiae "Weiszfeiler" no 52.

M. simiae "Weiszfeiler" strain No. 52 belongs to the second Runyon Group of Atypical Mycobacteria characterized by rough, eugonic, yellow-orange pellicle grown on Sauton medium incubated for 14 days at 37 degrees C. A suspension containing 10 mg/ml (equalling 5 times 10-7 viable units) injected intraveneously into rabbits provokes profound changes in the ultrastructure of the pulmonary tissue, manifested in activation of macrophages and pathologic alteration of pneumocytes. In histologic sections stained according to the Ziehl-Neelsen technique and examined in a light-microscope cytoplasmic acid-fast, highly dispersed granules are found in macrophages, resembling in morphological appearance the cellular phospholipids in sarcoid granuloma. Abundant production of interalveolar and interseptal fibrotic collagen tissue was observed in rabbits infected with M. Simiae a rather striking phenomenon in fresh experimental mycobacterial infection in the rabbit. The strain in question should therefore be considered not as Saprophyte but as a Mycobacterium Species with attenuated virulence, closely related to Prissic and Masson's M. scrophulaceum.

Animals↗