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L T Sennello

Publications and source records attributed to L T Sennello.

25 records · Page 2Linked to original sources
PubMed1976

High-pressure liquid chromatographic determination of 2-aminoethyl hydrogen sulfate in plasma and urine.

A sensitive and specific method for the analysis of 2-aminoethyl hydrogen sulfate in biological fluids by high-pressure liquid chromatography is described. Aliquots from deproteinized plasma or from acidified urine were subjected to ion-exchange clean-up by eluting with water through two columns packed with Dowex 1-X4 (Cl- form) and Dowex 50W-X8 (H+ form) resins. Recoveries in the eluates were consistent and quantitative (95-100%). Effluents from such treatment were chromatographed on a Durrum cation-exchange column with 0.05 M citrate buffer containing 0.15 N Na+ (pH 2.6). Detection was accomplished with an automated fluorescamine detection system. Using 2 ml of plasma or urine, 2-aminoethyl hydrogen sulfate could be assayed at concentrations of 0.3 mug/ml. Linear responses were observed up to at least 133 mug/ml. The method is both accurate and reproducible with a relative standard deviation of about +/- 4.0%.

Animals↗
PubMedDate not supplied

Kinetics of cefsulodin in patients with renal impairment.

Kinetics of cefsulodin were determined after administration of a 500-mg dose to normal subjects and patients with various degrees of renal insufficiency, including those requiring hemodialysis. Elimination kinetics were described by a two-compartment model. The steady-state volume of distribution was 0.26 liters/kg regardless of renal function. When the glomerular filtration rate (GFR) was greater than 80 ml/min, the elimination half-life (t1/2) was 1.9 hr. When the GFR ranged from 79 to 53 ml/min, t1/2 was 2.9 hr. In patients with moderate renal failure, in whom the GFR was 32-22 ml/min, t1/2 was 5.7 hr. In anuric patients, t1/2 was 13.0 hr. During hemodialysis the average plasma flow was 122 ml/min, dialyzer plasma clearance was 50.9 ml/min, concentration of drug in plasma was reduced by 60%, and t1/2 decreased to 2.1 hr. After dialysis the elimination rate appeared to return to that found in subjects not undergoing dialysis. While progressive renal failure slows the elimination of cefsulodin, there is a linear relationship between elimination of cefsulodin and GFR such that dosage nomograms can be developed. In patients undergoing hemodialysis, the maintenance dose of cefsulodin should be reduced to 10% of normal, and 60% of the dose should be given after hemodialysis.

Adult↗