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L T Thompson

Publications and source records attributed to L T Thompson.

22 records · Page 2Linked to original sources
PubMedDate not supplied

Trace eyeblink conditioning in rabbits demonstrates heterogeneity of learning ability both between and within age groups.

Rabbits 2 to 41 months of age were conditioned in the 500 ms trace eyeblink paradigm to cross-sectionally define the age of onset and the severity of age-associated impairments in acquisition of this relatively difficult hippocampally dependent task. Using a strict behavioral criterion of 80% conditioned responses (CRs), age-associated learning impairments were significant by 24 months of age. Among rabbits that successfully reached this criterion, impairments in acquisition plateaued at 30 months of age. However, the proportion of severely impaired rabbits (that failed to reach the 80% criterion) continued to increase age dependently. Using an easier criterion of 8 out of 10 CRs, behavioral impairments were not detected until 30 months of age, and cases of severe impairment (failure to reach criterion) were rare. Additional controls demonstrated that the deficits observed were not attributable to nonassociative changes that might have artifactually skewed the data. Even severely impaired 36-month-old rabbits were able to reach a criterion of 80% CRs when switched from a trace to a delay conditioning task that is not hippocampally dependent. The results are discussed in terms of operationally defining and predicting behavioral effects of aging, hypothetical neural mechanisms, and efficient experimental design.

Age Factors↗
PubMedDate not supplied

Age-related loss of calcium binding proteins in rabbit hippocampus.

Using immunocytochemistry hippocampal levels of the calcium binding proteins calbindin 28K (CB) and parvalbumin (PV) was studied in young (1 month) to very old (60 month) Albino rabbits. Young (3 month) and senescent (30 month) Wistar rats were also examined to compare the distribution and age dependency of PV and CB in both species. The distribution of PV-ir is similar in the rabbit and rat hippocampus. Aging in both species yielded a small loss of PV-ir in axon terminals. The presence of CB-ir interneurons throughout the hippocampus, and the heavy investment of the dentate gyrus (DG) granular cells with CB-ir was also similar in both species. In rabbits, the number of CB-ir interneurons in the CA1, as well as the density of CB-ir in the DG decreased in the first year of life, and did not change between 12-48 months of age. A secondary reduction in the density of CB-ir in the DG was observed at ages beyond 48 months. A similar loss of CB-ir in the DG occurred in the rat. In the CA1, however, the density of CB-ir was similar in young and aged rats. Another remarkable finding was the total absence of CB-ir in CA1 pyramidal neurons of rabbits at any age. Thus, the distribution and age dependency of PV-ir in the hippocampus is similar in both species. The decline of CB-ir in the DG with advancing age is very prominent and may be related to an altered calcium homeostasis in these cells. However, the absence of CB-ir in the CA1 of rabbits makes a causal role for CB in the functional decline of CA1 pyramidal cells during aging unlikely.

Age Distribution↗