Biomedical subjects

L Tůmová

Publications and source records attributed to L Tůmová.

16 recordsLinked to original sources

PubMed2006

[Effect of ultrasound on the production of flavonoids by the culture of Ononis arvensis L. in vitro].

The paper tested the effect of ultrasound of output density of 0.1 W/cm3 (stable frequency, 35 kHz) on the production of flavonoids in the suspension culture of Ononis arvensis L. The use of this abiotic elicitor was proved good to increase the production of secondary substances (flavonoids) in the culture of Ononis arvensis L. in vitro. The maximal production of flavonoids began after a 1 min action of ultrasound and immediate withdrawal of the culture--an increase by 677% in comparison with the control (without the action of ultrasound).

Fabaceae↗

PubMed2006

[Effect of AgNO3 on the production of flavonoids by the culture of Ononis arvensis L. in vitro].

The study tested the effect of abiotic elicitor, AgNO3, in different concentrations, on the production of flavonoids in the callus culture Ononis arvensis L. The use of this abiotic elicitor proved to be good to increase the production of flavonoids in in vitro culture. The maximal production was achieved after a 24-hour elicitation with AgNO3 in a concentration c1 (0.5 mg/1)--an increase by 934% versus the control (without the elicitor's action).

Cells, Cultured↗

PubMed2005

[Effect of vanadyl sulfate (VOSO4) on the production of flavonoids by the culture Ononis arvensis L. in vitro].

The study tested the effect of vanadyl (IV) sulfate (VOSO4) on the production of flavonoids in the callus and suspension culture of Ononis anrensis L. (Fabaceae). This substance proved to be a good abiotic elicitor for an increase in the production of secondary substances (flavonoids) in the culture of Ononis arvensis L. in vitro. The maximal increase in the formation of flavonoids took place in the callus culture after 24-hour elicitation with VOSO4 in a concentration of 1.227 .10-4 mol/l by 313% in comparison with the control and in the suspension culture in a concentration of 1.227 .10-6 mol/l after 48-hour action of the elicitor by 485% in comparison with the control.

Fabaceae↗

PubMed2004

[Silybum marianum in vitro].

Callus and suspension cultures of Silybum marianum were derived and the growth and production characteristics were assayed. The maximal production of flavonolignans was achieved at the beginning of cultivation, i.e. on day 10 after inoculation in MS medium with alpha-NAA and on day 15 after inoculation in MS medium with 2.4-D with continuous growth of the culture. The content of silymarin in the plant drug was 3.05%, in the in vitro culture it was just tenths of percents. TLC analysis revealed that the spectrum of flavonolignans of the drug differed from the spectrum of flavonolignans produced by tissue cultures. No differences in the spectra of flavonolignans produced by the callus and suspension cultures were observed. The test for antioxidative activity against the DPPH-radical demonstrated marked antioxidative properties of substances produced by the culture of Silybum marianum.

Antioxidants↗

PubMed2004

[Oxidative determination of rutin in tablets using flow injection analysis and chemiluminescence].

A simple, rapid and fully automated flow injection method (FIA) with chemiluminescence detection after oxidation with KMnO4 in acid medium at room temperature has been developed for the determination of routine in pharmaceutical formulation. Hexamethaphosphate sodium was utilized as enhancer of chemiluminescence. The calibration curve was linear in the range 0.01-0.26 mmol.l-1 with detection limit 0.005 mmol.l-1, RSD 0.46% (n = 10) and a sample throughput of 75.h-1 using a 200 microliters sample volume. The method was used for the determination of routine in mass-produced dosage form: Rutin 250 tablets á 50 mg, (firm Nature's Bounty, USA). The FIA method was statistically compared with the official German Pharmacopoeia method and showed comparable accuracy, but with the advantages of simplicity, speed and amounts of reagents consumed.

Flow Injection Analysis↗

PubMed2003

[Iodoacetic acid as a potential elicitor of increased production of flavonoids in a culture of Ononis arvensis L. in vitro].

The paper tested the effect of iodoacetic acid in four different concentrations on the production of flavonoids in the callus and suspension culture of Ononis arvensis L. The elicitor was in contact with the culture for a period of 6; 24; 47; 72, and 168 hours. The obtained results show a statistically significant increase in the content of flavonoids in the suspension culture in comparison with the control in the use of all tested concentrations of the elicitor after 6; 24; 48, and 72 hours of elicitation. The maximal increase by 586% in the production of flavonoids in the suspension culture took place with the use of iodoacetic acid in a concentration of 1 mg/l. In the callus culture, the maximal increase in the formation of flavonoids occurred with the use iodoacetic acid in a concentration of 10 mg/l after 24 hours of elicitation, the content of flavonoids being increased by 529% in comparison with the control.

Culture Techniques↗

PubMed2002

[Effect of CrCl3 on the in vitro production of flavonoids in a culture of Ononis arvensis L].

The paper investigated the effect of the abiotic elicitor chromium trichloride on the production of flavonoids in the callus and suspension cultures of Ononis arvensis L. after 12; 24; 48; 72, and 168-hour administration. The tested elicitor positively influenced the formation of flavonoids. A statistically significant increase in the flavonoid production in the callus culture was recorded with the use of a chromium trichloride solution in the concentration c1 (6.32 x 10(-4) mol/l) after 24; 48; 72, and 168 hours, in the concentration c2 (6.32 x 10(-6) mol/l) after 24; 48, and 72 hours, and in the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24, and 48 hours. In the suspension culture, the flavonoid production was significantly increased by the concentration c1 (6.32 x 10(-4) mol/l) after 12 and 72 hours, the concentration c2 (6.32 x 10(-6) mol/l) after 12; 24; 48, and 72 hours, and the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24; 48, and 72 hours. The maximal increase in the flavonoid production by 98% took place in the callus culture after elicitation with a chromium trichloride in the concentration c3 (6.32 x 10(-8) mol/l) after 48 hours of administration, and in the suspension culture after elicitation with CrCl3 in the concentration c2 (6.32 x 10(-6) mol/l) after 12 hours by 100%.

Chlorides↗

PubMed2002

[Effect of jasminic acid on production of flavonoids in a culture of Ononis arvensis L. in vitro].

The present paper examined the effect of jasminic acid in three different concentrations (1000 mg/l; 10 mg/l, and 0.1 mg/l) on the formation of flavonoids in the culture Ononis arvensis L. in vitro. The tested concentrations of jasminic acid were applied to the callus and suspension cultures of Ononis arvensis L. in the 4th week of culture growth and elicitation took place for 12; 24; 48; 72, and 168 hours. A statistically significant increase in the content of flavonoids was found in all administered solutions of jasminic acid after 12 hours of elicitation, excepting the concentration c2 (10 mg/l) in the suspension culture. The highest yield was a 55% increase in the content of flavonoids after elicitation with jasminic acid in the concentration c1 (1000 mg/l) in the callus culture.

Culture Techniques↗

PubMed2002

[Ononis arvensis in vitro--abiotic elicitation].

The effect of two abiotic elicitors--two newly synthesized compounds (substituted anilides of pyrazine-2-carboxylic acids)--on the formation of flavonoids in the callus culture of Ononis arvensis L. was tested. The compounds markedly influenced the production of flavonoids in an in vitro culture. Particularly after elicitation with a solution of compound No. 1 (4-hydroxyanilide 6-chloro-5-terc.butylpyrazine-2-carboxylic acid) in a concentration of 3.32.10(-7) mol.l-1 and within 48 hours of elicitation an increase in flavonoid formation by 976% versus the control took place.

Anilides↗

PubMed2000

[Effect of linoleic acid on the production of secondary metabolites].

The paper examines the effect of linoleic acid in various concentrations on the production of flavonoids in an Ononis arvensis culture in vitro and the production of anthracene derivatives in a Rheum palmatum culture in vitro. The content of the metabolites under study after elicitation with linoleic acid, which is the precursor of jasminic acid intervening into the course of elicitation, was examined after 12; 24; 48 and 168 hours. The maximal increase in the content of flavonoids (118%) was found after the application of linoleic acid in a concentration of 2 mg/ml after 48 hrs. In concentrations of 0.01; 0.20, and 1 mg/ml after 48 hours of application of the elicitor, the highest increase in the content of flavonoids was observed after 24 hrs in a concentration of 2.0 mg/ml. In the elicitation of the Rheum palmatum culture, the highest response (100%) in the production of anthracene derivatives was observed in linoleic acid in a concentration of 1 mg/ml.

Anthracenes↗

PubMed2000

[Interactions between herbal medicines and drugs].

At present the use of medicaments of plant origin is on the increase. It is therefore necessary to take into consideration that there exist known as well as potential interactions between the medicament of the medicinal plant. The problematic plants include Echinacea, Allium cepa, Gingko biloba, Panax ginseng, as well as Hypericum perforatum, Valeriana officinalis, or Glycyrrhiza glabra. Its use should be limited, or completely excluded in the cases of simultaneous therapy with, e.g., warfarin, hepatotoxically acting medicaments, MAOI inhibitors, phenelzin sulphate, or phenytoin, as they may decrease of completely eliminate the therapeutic effect of the administered drugs, or they may cause a toxic damage to the organism.

Drug Interactions↗

PubMed2000

[Allergic contact dermatitis caused by propolis].

The present paper describes several individual cases of contact dermatitis in patients using propolis as a component of various cosmetic products, listing the most frequently sensitizing constituents of propolis. It also reports the existence of a cross-reaction between the components of Peruvian balsam and propolis constituents.

Allergens↗

PubMed1999

[The effect of elicitors from Pseudomonas aeruginosa on the production of flavonoids in cultures of Ononis arvensis L].

The effect of the elicitor, killed cells of Pseudomonas aeruginosa, on the production of flavonoids by a callus and a suspension culture of Ononis arvensis L. was examined. The tested elicitor influenced the flavonoid production both in the callus and suspension culture. An increased content of flavonoids in the callus culture was observed with the use of the elicitor in concentrations I and II (5.81 x 10(-2) and 5.81 x 10(-4) g/l). The maximal production took place after a seven-day elicitation with concentration II. This production was higher by 83% as compared with the control. In suspension cultures an increased production was observed in concentrations I and III (5.81 x 10(-2) and 5.81 x 10(6) g/l). The maximal content of flavonoids was measured after 48-hour elicitation with concentration I. In comparison with the control the increase in the flavonoid content was higher by up to 125%.

Culture Techniques↗

PubMed1996

[Antineoplastic effects of natural substances].

The present paper surveys natural substances with antitumour activity, particularly primary metabolites--polysaccharides, lectins and lipids tested in vitro or on laboratory animals. The presented data cover the period of 1990-1995.

Adult↗

PubMed1995

[Flavonoids in the prevention of cancer].

The paper summarizes the possibilities of using flavonoids in the treatment and especially prevention of human cancer. It also describes the probable mechanism of protective actions of food flavonoids.

Diet↗

PubMed1995

[Natural substances causing allergies and dermatitis].

On the basis of the literature search from the journal Chemical Abstracts (1988-1992) the present paper processes the data about 142 plants from 49 families which are the causes of the development of contact dermatitides. The paper also lists the allergens responsible for the development of contact dermatitides.

Allergens↗