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Biomedical subjects

L Tay

Publications and source records attributed to L Tay.

43 records · Page 3Linked to original sources

A prospective study of group B streptococcal colonization in parturient mothers and their infants.

The objective of this study was to investigate the rates of group B streptococcal colonization among parturients within the 24 hours prior to delivery and colonization of their newborns. It was also to identify high risk factors. 204 parturients were randomly selected in the Alexandra Hospital, Singapore. Swabs for culture were taken from the maternal vagina and throat and the neonate's ears, throat and umbilicus. In this study it was found that vaginal colonization in the mother is significantly associated with neonatal colonization. Among the various ethnic groups, Malays and Indians/Pakistanis are at a higher risk of having vaginal colonization. All other factors studied, however, failed to show any influence on neonatal colonization and thus appear to have had no forewarning value.

Adult↗

Development of an immunoassay for BMS-191352, a single-chain immunotoxin, and its application to toxicokinetic studies.

BMS-191352 is a single-chain fusion protein composed of the variable regions of chimeric BR96 monoclonal antibody and the binding defective form of Pseudomonas Exotoxin A (PE40). The immunotoxin exhibits potent cytotoxicity against tumor cells expressing the Lewis antigen. A sensitive and specific double antibody sandwich ELISA has been developed and validated for the determination of BMS-191352 in rat and dog EDTA plasma. A monoclonal anti-PE40 antibody (EXA2-1H8) was used to capture BMS-191352 in plasma samples. The captured BMS-191352 was then detected using a biotinylated monoclonal BR96 antiidiotypic antibody (757-4-1) followed by the addition of streptavidin-horseradish peroxidase conjugate and chromogen 3,3',5,5'-tetramethylbenzidine. The optical density was measured at 450 nm. The standard curve range in rat and dog plasma was 2-32 ng/mL. The RSD for the inter- and intra-assay precision was within 9.2% and the accuracy was greater than 89.0%. The ELISA method was applied to the analysis of BMS-191352 in plasma samples from toxicokinetic studies conducted in rats and dogs. These studies revealed that the systemic exposure of BMS-191352 was dose proportional and the kinetics of BMS-191352 were linear between the dose range of 1.8-7.2 mg/m2 in the dog.

ADP Ribose Transferases↗